RT  - Journal of Clinical Pediatric Dentistry
ID  - 10.17796/jcpd.30.3.q1561155x22u0774
T1  - Black stains in the mixed dentition: A PCR microbiological study of the etiopathogenic bacteria
A1  - C. Saba
A1  - M. Solidani
A1  - F. Berlutti
A1  - A.Vestri
A1  - L. Ottolenghi
A1  - A. Polimeni
YR  - 2006
SP  - 219
AB  - <p>The aim of this work is to emphasize that particular stains on the third cervical of the buccal and lin-gual surfaces in mixed dentition, called “black stain.” Previous research showed the microbiological etiology of this discoloration by chromogen bacterias. Our study shows bacteria spp involved in stains by means of PCR process and electrophoresis gel on the agarose medium. Sample was formed by 100 subject with black stain and 100 control subjects stain-free. A statistical analysis (SPSS 10.0) using X2 was performed in this study. Porphyromonas gingivalis and Prevotella melaninogenica, were not involved in both in black stain subjects and in the control. On the contrary, Actinomyces could be involved in the pigmentation process.</p>