Background: This cross-sectional study was to explore associations between children’s dental anxiety (CDA) and parental acceptance of Dental General Anesthesia (DGA) in Chongqing, China and to provide relevant clinical recommendations. Methods: The study was conducted among 743 parent-child couples. Demographic characteristics, DGA knowledge of parents and parental acceptance of DGA was collected by a self-designed questionnaire. CDA was measured by the Chinese version of the Modified Dental Anxiety Scale (MDAS). Results: More than 70% of the parents were unwilling to accept DGA. After controlling demographic factors, DGA knowledge of parents (odds ratio (OR) = 1.23, 95% confidence interval (CI): 1.12–1.35), parents’ having heard of DGA (OR = 1.23, 95% CI: 1.12–1.35), and children’s experience of general anaesthesia (GA) was more likely (OR = 1.23, 95% CI: 1.12–1.35) to promote parents to accept DGA. Compared to parents of children with medium anxiety (OR = 0.58, 95% CI: 0.36–0.94), parents of children with high or no CDA were more likely to accept DGA. Among children with high CDA, girls’ parents (OR = 2.77; 95% CI: 1.27–6.09), parents whose children had GA experience (OR = 18.78, 95% CI: 4.5–78.05) and fathers with clerical or skilled workers occupation (OR = 3.59, 95% CI: 1.08–11.93) were more likely to accept DGA than others, however, mothers with freelance work (OR = 0.13, 95% CI: 0.03–0.53) and families with low monthly annual household income (<¥5000) were less likely to accept DGA than others. Conclusions: These findings might inform the development of targeted interventions to manage children’s dental anxiety.