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Original Research

Open Access

Longitudinal Evaluation of Salivary Iga-S in Children with Early Childhood Caries Before and After Restorative Treatment

  • Aline dos Santos Letieri1
  • Liana Bastos Freitas-Fernandes1
  • Ana Paula Canedo Valente2,*,
  • Tatiana Kelly da Silva Fidalgo3
  • Ivete Pomarico Ribeiro de Souza1

1Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Brazil

2National Center for Nuclear Magnetic Resonance, Medical Biochemistry, Universidade Federal do Rio de Janeiro, Brazil

3Department of Preventive and Community Dentistry, Universidade do Estado do Rio de Janeiro, Brazil

DOI: 10.17796/1053-4625-43.4.3 Vol.43,Issue 4,July 2019 pp.239-243

Published: 01 July 2019

*Corresponding Author(s): Ana Paula Canedo Valente E-mail: valente@cnrmn.bioqmed.ufrj.br

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Abstract

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s > 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p<0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p>0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p<0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p>0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.

Keywords

Saliva; Dental caries; Immunoglobulin A; Child, Preschool; Streptococcus mutans; Lactobacillus spp.


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Aline dos Santos Letieri,Liana Bastos Freitas-Fernandes,Ana Paula Canedo Valente,Tatiana Kelly da Silva Fidalgo,Ivete Pomarico Ribeiro de Souza. Longitudinal Evaluation of Salivary Iga-S in Children with Early Childhood Caries Before and After Restorative Treatment. Journal of Clinical Pediatric Dentistry. 2019. 43(4);239-243.

URL:

https://www.jjocpd.com/articles/10.17796/1053-4625-43.4.3

References

1. Misra S, Tahmassebi JF, Brosnan M. Early childhood caries—a review. Dent Update;34(9):556-8, 61-2, 64. 2007.

2. Dye BA, Hsu KL, Afful J. Prevalence and Measurement of Dental Caries in Young Children. Pediatr Dent;37(3):200-16. 2015.

3. Garcia R, Borrelli B, Dhar V, et al. Progress in Early Childhood Caries and Opportunities in Research, Policy, and Clinical Management. Pediatr Dent;37(3):294-9. 2015.

4. Policy on Early Childhood Caries (ECC): Classifications, Consequences, and Preventive Strategies. Pediatr Dent;39(6):59-61. 2017.

5. Harris R, Nicoll AD, Adair PM, Pine CM. Risk factors for dental caries in young children: a systematic review of the literature. Community Dent Health;21(1 Suppl):71-85. 2004.

6. Scully C. Dental caries: progress in microbiology and immunology. J Infect;3(2):107-33.1981.

7. Smith DJ, Shoushtari B, Heschel RL, King WF, Taubman MA. Immunogenicity and protective immunity induced by synthetic peptides associated with a catalytic subdomain of mutans group streptococcal glucosyltransferase. Infect Immun;65(11):4424-30. 1997.

8. Wallengren ML, Hamberg K, Ericson D, Nordberg J. Low salivary IgA activity to cell-surface antigens of mutans streptococci related to HLA-DRB1*04. Oral Microbiol Immunol;20(2):73-81. 2005.

9. Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev;62(1):71-109. 1998.

10. Hajishengallis G, Nikolova E, Russell MW. Inhibition of Streptococcus mutans adherence to saliva-coated hydroxyapatite by human secretory immunoglobulin A (S-IgA) antibodies to cell surface protein antigen I/II: reversal by IgA1 protease cleavage. Infect Immun;60(12):5057-64. 1992.

11. Bagherian A, Asadikaram G. Comparison of some salivary characteristics between children with and without early childhood caries. Indian J Dent Res;23(5):628-32. 2012.

12. Chawda JG, Chaduvula N, Patel HR, Jain SS, Lala AK. Salivary SIgA and dental caries activity. Indian Pediatr;48(9):719-21. 2010.

13. Chopra M, Jadhav S, Venugopalan A, Hegde V, Chopra A. Salivary immunoglobulin A in rheumatoid arthritis (RA) with focus on dental caries: a cross-sectional study. Clin Rheumatol;31(2):247-50. 2011.

14. Hagh LG, Zakavi F, Ansarifar S, Ghasemzadeh O, Solgi G. Association of dental caries and salivary sIgA with tobacco smoking. Aust Dent J;58(2):219-23. 2013.

15. Pal S, Mitra M, Mishra J, Saha S, Bhattacharya B. Correlation of total salivary secretory immunoglobulin A (SIgA) and mutans specific SIgA in children having different caries status. J Indian Soc Pedod Prev Dent;31(4):270-4. 2013.

16. Priya PR, Asokan S, Karthick K, Reddy NV, Rao VA. Effect of dental treatments on salivary immunoglobulin A of children with and without dental caries: a comparative study. Indian J Dent Res;24(3):394. 2013.

17. Kirtaniya BC, Chawla HS, Tiwari A, Ganguly NK, Sachdev V. Natural prevalence of antibody titres to GTF of S. mutans in saliva in high and low caries active children. J Indian Soc Pedod Prev Dent;27(3):135-8. 2009.

18. Fidalgo TK, Freitas-Fernandes LB, Ammari M, et al. The relationship between unspecific s-IgA and dental caries: a systematic review and meta-analysis. J Dent;42(11):1372-81. 2014.

19. World Health Organization. Oral health surveys: basic methods. World Health Organization, 2013.

20. Fidalgo TKS, Freitas-Fernandes LB, Almeida FL, Valente AP, Souza IPR. Longitudinal evaluation of salivary profile from children with dental caries before and after treatment. Metabolomics;11(3):780-85. 2015.

21. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem;72:248-54. 1976.

22. Parisotto TM, King WF, Duque C, et al. Immunological and microbiologic changes during caries development in young children. Caries Res;45(4):377-85. 2011.

23. Ranadheer E, Nayak UA, Reddy NV, Rao VA. The relationship between salivary IgA levels and dental caries in children. J Indian Soc Pedod Prev Dent;29(2):106-12. 2011.

24. Takahashi N, Nyvad B. The role of bacteria in the caries process: ecological perspectives. J Dent Res;90(3):294-303. 2011.

25. Parisotto TM, Steiner-Oliveira C, Silva CM, Rodrigues LK, Nobre-dosSantos M. Early childhood caries and mutans streptococci: a systematic review. Oral Health Prev Dent;8(1):59-70. 2010.

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