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Role of Epithelial Mesenchymal Transition in Phenytoin Influenced Gingival Overgrowth in Children and Young Adults. A Preliminary Clinical and Immunohistochemical Study

  • Srirangarajan Sridharan1,*,
  • Priyanka Nunna1
  • Tina Elizabeth Jaicob 2
  • Dominic Augustine2
  • Varun Shetty 1
  • Divya Srirangarajan3

1Department of Periodontics, Bangalore Institute of Dental Sciences and Postgraduate Research Centre, 5/3, Hosur Road, Bangalore, India

2Department of Oral and Maxillofacial Pathology, Bangalore Institute of Dental Sciences and Post graduate research center, Bangalore, India

3Department of Pediatrics, ASTER CMI health care private limited, Bangalore, India

DOI: 10.17796/1053-4625-43.5.9 Vol.43,Issue 5,September 2019 pp.350-355

Published: 01 September 2019

*Corresponding Author(s): Srirangarajan Sridharan E-mail: : docranga@yahoo.com

Abstract

Objectives: To prove the role of epithelial mesenchymal transition (EMT) in the pathogenesis of phenytoin influenced gingival overgrowth (PIGO) in children and young adults. Study design: Thirty male individuals who are to start with oral phenytoin therapy were recruited for the study. All the 30 individuals underwent full mouth scaling and root planning and were then followed up for a period of one year at intervals of 3 months each. Based on the clinical gingival status they were divided into group1 (responders) individuals who showed gingival overgrowth (GO) and group 2 (non responders) individuals who do not show any GO. Gingival tissue samples were obtained from both the groups at the end of 1 year and subjected to immuno histochemical analysis for E-cadherin expression and histo-pathological for alteration in the basement membrane and confirmation of the fibrosis. Results: Decrease in expression of E cadherin, loss of basement membrane integrity and fibrosis were noted on responder group when compared to non responder group at p<0.001. Fibrosis was seen in the epithelial connective tissue junction. Conclusion: Decrease in cell adhesion, degradation of basement membrane and presence of fibrosis could suggest the role of EMT in the pathogenesis of PIGO.


Keywords

Epithelial Mesenchymal Transition; Gingival overgrowth; Phenytoin; Collagen; Fibroblast.

Cite and Share

Srirangarajan Sridharan,Priyanka Nunna,Tina Elizabeth Jaicob ,Dominic Augustine,Varun Shetty ,Divya Srirangarajan. Role of Epithelial Mesenchymal Transition in Phenytoin Influenced Gingival Overgrowth in Children and Young Adults. A Preliminary Clinical and Immunohistochemical Study. Journal of Clinical Pediatric Dentistry. 2019. 43(5);350-355.

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