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Regenerative Endodontic Therapy with Platelet Rich Fibrin: Case Series

  • G Kandemir Demirci1,*,
  • P Güneri2
  • MK Çalışkan1

1Department of Endodontology, School of Dentistry, Ege University, İzmir, Turkey

2Department of Oral and Maxillofacial Radiology, School of Dentistry, Ege University, İzmir, Turkey

DOI: 10.17796/1053-4625-44.1.3 Vol.44,Issue 1,January 2020 pp.15-19

Published: 01 January 2020

*Corresponding Author(s): G Kandemir Demirci E-mail: dt.gozdekandemir@hotmail.com

Abstract

Regenerative endodontic therapy (RET) provides a novel treatment modality for the immature teeth with pulp necrosis. The aim of this case series was to evaluate RET of immature permanent teeth using platelet rich fibrin (PRF) at 36-month follow-up periods. In the present case series, three immature maxillary incisors diagnosed with pulp necrosis and apical periodontitis were treated with RET. The root canals were irrigated with 1.5% sodium hypochlorite (NaOCl) and medicated with triple antibiotic paste(TAP). At the second visit, TAP was removed and root canals were conditioned with 17% EDTA. PRF was used as a scaffold. MTA was placed over PRF and the teeth were restored with composite resin. Periapical radiographs and cone beam computerized tomography(CBCT) were used to evaluate the healing. At the end of the 36-month follow-up periods, there was no response to pulp sensibility tests with cold and electric pulp tester, but all teeth showed decreased periapical lesions or evidence of healing.


Keywords

Immature permanent tooth; Platelet rich fibrin; Regenerative endodontic therapy


Cite and Share

G Kandemir Demirci,P Güneri,MK Çalışkan. Regenerative Endodontic Therapy with Platelet Rich Fibrin: Case Series. Journal of Clinical Pediatric Dentistry. 2020. 44(1);15-19.

References

1. Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: A review of current status and a call for action. J Endod; 33: 377-90. 2007.

2. Kontakiotis EG, Filippatos CG, Tzanetakis GN, Agrafioti A. Regenerative endodontic therapy: A data analysis of clinical protocols. J Endod; 41; 146-54. 2015.

3. Banchs F, Trope M. Revascularization of immature permanent teeth with apical periodontitis: New treatment protocol? J Endod 30; 196-200, 2004.

4. Torabinejad M, Turman M. Revitalization of tooth with necrotic pulp and open apex by using platelet-rich plasma: A case report. J Endod; 37:265-8. 2011.

5. Bezgin T, Yılmaz AD, Celik BN, Kolsuz ME, Sonmez H. Efficacy of platelet-rich plasma as scaffold in regenerative endodontic treatment. J Endod; 41, 36-44. 2015.

6. Keswani D, Pandley RK. Revascularization of an immature tooth with a necrotic pulp using platelet-rich fibrin: a case report. Int Endod J; 46: 1096- 104. 2013.

7. Mishra N, Narang I, Mittal N. Platelet rich fibrin mediated revitalization of immature necrotic tooth. Contemp Clin Dent; 4: 412-5. 2013.

8. Ray HL, Marcelino J, Braga R, Horwat R, Lisien M, Khaliq S. Long-term follow up of revascularization using platelet-rich fibrin. Dent Traumatol; 32: 80-4. 2016;

9. Shivashankar VY, Johns DA, Vidyanath S, Kumar MR. Platelet rich fibrin in the revitalization of tooth with necrotic pulp and open apex. J Conserv Dent; 2012:15: 395-8.

10. Nurden AT, Nurden P, Sanchez M, Andia I, Anitua E. Platelets and wound healing. Front Biosci; 13: 3532-48. 2008.

11. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, Dohan AJ, Mouhyi J, Dohan DM. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod; 101: e56-60. 2006.

12. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod; 101: e37-44. 2006.

13. Safi L, Ravanshad S. Continued root formation of a pulpless permanent incisor following root canal treatment: A case report. Int Endod J; 38:489- 93. 2005.

14. Huang FM, Yang SF, Zhao JH, et al. Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells. J Endod; 36: 1628-32. 2010.

15. American Association of Endodontics. AAE Clinical considerations for a regenerative procedure. American Association of Endodontists; 2016. Available from: http://www.aae.org/uploadedfiles/publications_and_ research/research/currentregenerativeendodonticconsiderationns.pdf.

16. Estefan BS, El Batouty KM, Nagy MM, Diogenes A. Influence of age and apical diameter on the success of endodontic regenerative procedures. Endod J; 42: 1620-5. 2016.

17. Saoud TMA, Zaazou A, Nabil A, Moussa S, Lin LM, Gibbs JL. Clinical and radiographic outcomes of traumatized immature permanent necrotic teeth after revascularization/revitalization therapy. J Endod; 40: 1946-52. 2014.

18. Lin J, Zeng Q, Wei X, Zhao W, Cui M, Gu J, Lu J, Yang M, Ling J. Regenerative endodontics versus apexification in immature permanent teeth with apical periodontitis: a prospective randomized controlled study. J Endod: 43: 1821-7. 2017.

19. Kim SG, Malek M, Sigurdsson A, Lin LM, Kahler B. Regenerative endodontics: a comprehensive review. Int Endod J; 51: 1367-1388. 2018

20. Martin DE, Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A. Concentration-dependent effect of sodium hypoclorite on stem cells of apical papilla survival and differentiation. J Endod ; 40: 51-5. 2014.

21. Trevino EG, Patwardhan AN, Henry MA, Perry G, Dybdal-Hargreaves N, Hargreaves KM, Diogenes A. Effect of irrigants on survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. J Endod; 37:1109-15. 2011.

22. Torabinejad M, Faras H, Corr R, Wright KR, Shabahang S. Histologic examinations of teeth treated with 2 scaffolds: a pilot animal investigation. J Endod; 2014:40: 515-20.

23. Smith AJ, Duncan HF, Diogenes A, Simon S, Cooper PR. Exploiting the bioactive properties of the dentin-pulp complex in regenerative endodontics. J Endod; 42: 47-56. 2016.

24. Galler KM, Buchalla W, Hiller KA, Federlin M, Eidt A, Schiefersteiner M, Schmalz G. Influence of root canal disinfectants on growth factor release from dentin. J Endod ;2015:41: 363-8.

25. Windley W, Teixeira F, Levin L, Sigurdsson A, Trope M. Disinfection of immature teeth with a triple antibiotic paste. J Endod ; 31:439-43. 2015.

26. Ding RY, Cheung GSP, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod; 35: 745–9. 2009.

27. Petrino JA, Boda KK, Shambarger S, Bowles WR, McClanahan SB. Challenges in regenerative endodontics: a case series. J Endod; 36: 536–41. 2010.

28. Narang I, Mittal N, Mishra N. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: A clinical study. Contemp Clinic Dent; 56:63-8. 2011.

29. Toffler M, Toscano N, Holtzclaw D, Corso M, Ehrenfest DD. Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery milieu. J Implant Adv Clin Dent; 1: 21–30, 2009.

30. Diogenes A, Ruparel NB. Regenerative endodontic procedures: clinical outcomes. Dent Clin North Am; 61; 111-25. 2017

31. Caliskan MK. Success of pulpotomy in the management of hyperplastic pulpitis. Int Endod J ;1993:26: 142- 8.

32. Johns DA, Vidyanath S. Revitalization of tooth with necrotic pulp and open apex by using platelet-rich plasma. a case report. J Endod 37: 743-4, 2011.

33. Becerra P, Ricucci D, Loghin S, Gibbs JL, Lin LM. Histologic study of a human immature permanent premolar with choronic apical abscess after revascularization/revitalization. J Endod ; 40: 133-9. 2014.

34. Bakhtiar H, Esmaeili S, Fakhr Tabatabayi S, Ellini MR, Nekoofar MH, Dummer PMH. Second generation platelet-concentrate (Platelet-rich fibrin) as a scaffold in regenerative endodontics: a case series. J Endod: 43: 401-8. 2017.

35. Linsuwanont P, Sinpitaksakul P, Lertsakchai P. Evaluation of root maturation after revitalization in immature permanent teeth with nonvital pulp by cone beam computed tomography and conventional radiographs. Int Endod J; 50: 836-846. 2017

36. Zhu W, Zhu X, Huang GTJ, Cheung GSP, Dissanayaka WL, Zhang C. Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells. Int Endod J ; 46: 962-70. 2013.

37. Martin DE, Almeida JS, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A. Concentration-dependent effect of sodium hypoclorite on stem cells of apical papilla survival and differentiation. J Endod; 2014:40: 51-5.

38. Zhang DD, Chen X, Bao ZF, Chen M, Ding ZJ, Zhong M. Histological comparison between platelet-rich plasma and blood clot in regenerative endodontic treatment: an animal study. J Endod; 2014:40: 1388-93.

39. Torabinejad M, Milan M, Shabahang S, Wright KR, Faras H. Histological examination of teeth with necrotic pulps and periapical lesions treated with 2 scaffolds: an animal investigation. J Endod ; 41: 846-52. 2015.


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