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Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System

  • E Calvano Küchler1,2,*,
  • C Maschietto Pucinelli1
  • K Carpio Horta1
  • R Assed Bezerra da Silva1
  • M de Castro Costa3
  • A Rezende Vieira4
  • P Nelson-Filho1
  • L Assed Bezerra da Silva1
  • L Santos Antunes5
  • L Azeredo Antunes5

1Department of Pediatric Dentistry,School of Dentistry of Ribeirão Preto, University of São Paulo,Ribeirão Preto-Brazil

2School of Health Sciences, Positivo University,Curitiba-Brazil

3Department of Pediatric Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro-Brazil

4Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA

5Department of Specific Formation, School of Dentistry, Fluminense Federal University, Rio de Janeiro-Brazil

DOI: 10.17796/1053-4625-44.1.6 Vol.44,Issue 1,January 2020 pp.35-40

Published: 01 January 2020

*Corresponding Author(s): E Calvano Küchler E-mail:


Purpose: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness. Study design: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%. Results: Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05). Conclusion: The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.


Caries; Enamel; Polymorphism; Gene

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E Calvano Küchler,C Maschietto Pucinelli,K Carpio Horta,R Assed Bezerra da Silva,M de Castro Costa,A Rezende Vieira,P Nelson-Filho,L Assed Bezerra da Silva,L Santos Antunes,L Azeredo Antunes. Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System. Journal of Clinical Pediatric Dentistry. 2020. 44(1);35-40.


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