Title
Author
DOI
Article Type
Special Issue
Volume
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Antimicrobial Effect of Propolis Administered through Two Different Vehicles in High Caries Risk Children: A Randomized Clinical Trial
1Pediatric Dentistry Department, Faculty of Dentistry, Benghazi University, Libya
2Pediatric Dentistry, Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Alexandria University, Egypt
3Microbiology and Immunology Department, Faculty of Pharmacy, Alexandria University, Egypt
DOI: 10.17796/1053-4625-44.5.1 Vol.44,Issue 5,September 2020 pp.289-295
Published: 01 September 2020
*Corresponding Author(s): Dalia M Talaat E-mail: daliatalaat7567@hotmail.com Dalia.talaat@dent.alex.edu.eg
Objective: To investigate the effect of two methods of propolis administration on plaque accumulation and microbial count as well as patient acceptance of each vehicle. Study design: A randomized clinical trial with two parallel arms was used with a sample of 60 high caries risk children 6-8 years old. Children were divided randomly into two groups. Group I: Children who received propolis chewing gum and instructed to chew it twice daily for at least twenty minutes, for two weeks. Group II: children who received propolis mouthwash and instructed to rinse twice daily for one minute. A plaque index was recorded and a plaque sample was collected from all participants at base line and after two weeks of treatment. All participants were asked to rate the preparation they received during treatment period on a Visual Analogue Scale chart. Results: Data showed that propolis had a significant effect on reducing plaque scores and colony counts in both vehicles. There was no significant difference between both vehicles neither on plaque reduction nor on microbial count. However children preferred the gum formula. Conclusion: Propolis in both vehicles reduced plaque accumulation and microbial count which recommends its use as an antimicrobial agent in different vehicles.
Propolis; Mouthwash; Chewing Gum; Antimicrobial Agent; High Caries Risk Children
Hend S El-Allaky,Nadia A Wahba,Dalia M Talaat,Azza S Zakaria. Antimicrobial Effect of Propolis Administered through Two Different Vehicles in High Caries Risk Children: A Randomized Clinical Trial. Journal of Clinical Pediatric Dentistry. 2020. 44(5);289-295.
1. Li J, Helmerhorst EJ, Leone CW, Troxler RF, Yaskell T, Haffajee AD, et al. Identification of early microbial colonizers in human dental biofilm. J Appl Microbiol;97(6):1311–18. 2004.
2. Seneviratne CJ, Zhang CF, Samaranayake LP. Dental Plaque Biofilm in oral Health and Disease. Chinese J Dent Res;14(2):87–94. 2011.
3. Coelho Leal S, Barreto Bezerra AC, Ayrton de Toledo O. Effectiveness of teaching methods for toothbrushing in preschool children. Braz Dent J;13(2):133–6.2002.
4. Grover D, Kaur G, Kaushal S, Malhotra R. Toothbrush ′A key to mechanical plaque control′. Indian J Oral Sci;3(2):62-8. 2012.
5. Gunsolley JC. A meta-analysis of six-month studies of antiplaque and antigingivitis agents. JADA;137(12):1649–57.2006.
6. Axelsson P, Albandar JM, Rams TE. Prevention and control of periodontal diseases in developing and industrialized nations. Periodontol 2000; 29(1):235–46. 2002.
7. Serrano J, Escribano M, Roldan S, Martin C, Herrera D. Efficacy of adjunctive anti-plaque chemical agents in managing gingivitis: A systematic review and meta-analysis. Vol. 42, J Clin Periodontol. Apr; 42 Suppl 16:S106-38.2015.
8. Figuero E, Nóbrega DF, García-Gargallo M, Tenuta LM, Herrera D, Carvalho JC, Mechanical and chemical plaque control in the simultaneous management of gingivitis and caries: a systematic review, J. Clin. Periodontol. Mars; 44: Suppl 18: S116–S134. 2017.
9. Van der Weijden FA, Van der Sluijs E, Ciancio SG,. Slot DE. Can Chemical Mouthwash Agents Achieve Plaque/Gingivitis Control?, Dent. Clin. North Am; 59(4): 799–829. 2015.
10. James P, Henry W, Parnel C, Harding M, Lamont T, Cheung A, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health, Cochrane Database Syst. Rev. 2017 . doi:10.1002/14651858.CD008676. pub2.
11. Van Strydonck DAC, Slot DE, Van Der Velden U, Van Der Weijden F. Effect of a chlorhexidine mouthrinse on plaque, gingival inflammation and staining in gingivitis patients: A systematic review. J Clin Periodontol ;39(11):1042–55 . 2012.
12. Kumar S, Patel S, Tadakamadla J, Tibdewal H, Duraiswamy P, Kulkarni S. Effectiveness of a mouthrinse containing active ingredients in addition to chlorhexidine and triclosan compared with chlorhexidine and triclosan rinses on plaque, gingivitis, supragingival calculus and extrinsic staining. Int J Dent Hyg;11(1):35–40. 2013.
13. Nair AA, Malaiappan S. The Comparison of the antiplaque effect of Aloe Vera , chlorhexidine and placebo mouth washes on gingivitis patients. J. Pharm. Sci&Res; 8(11): 1295–300. 2016.
14. Wiȩckiewicz W, Miernik M, Wiȩckiewicz M, Morawiec T. Does propolis help to maintain oral health? Evidence-based Complement Altern Med. 2013;2013.
15. Betances-Salcedo E, Revilla I, Vivar-Quintana A, González-Martín M. Flavonoid and antioxidant capacity of propolis prediction using near infrared spectroscopy. Sensors.;17(7):1647. 2017.
16. Mossalayi MD, Rambert J, Renouf E, Micouleau M, Mérillon JM. Grape polyphenols and propolis mixture inhibits inflammatory mediator release from human leukocytes and reduces clinical scores in experimental arthritis. Phytomedicine 15;21(3):290–97. 2014.
17. Miguel MG, Antunes MD. Is propolis safe as an alternative medicine? J Pharm Bioallied Sci ;3 (4):479–95. 2011.
18. Waldner-Tomic N, Vanni R, Belibasakis G, Thurnheer T, Attin T, Schmidlin P. The in vitro antimicrobial efficacy of propolis against four oral pathogens: a review. Dent J; 2(3):85–97. 2014,
19. Savita AM, Devi P, Varghese A, Prerana GK. Evaluation of Clinical Efficacy of Propolis in Patients with Gingivitis: A Randomized Clinical Crossover Study. ASDS;2(8):75-80. 2018.
20. Ercan N, Erdemir EO, Ozkan SY, Hendek MK. The comparative effect of propolis in two different vehicles; mouthwash and chewing-gum on plaque accumulation and gingival inflammation. Eur J Dent; 9(2):272–6. 2015.
21. Steinberg LM, Odusola F, Mandel ID. Remineralizing potential, antiplaque and antigingivitis effects of xylitol and sorbitol sweetened chewing gum. Clin Prev Dent; 14(5):31–4.1992.
22. American Academy of Pediatric Dentistry, Guideline on Caries-risk Assessment and Management for Infants, Children, and Adolescents, Clin. Guidel. Ref. Man. 2015-2016.; 37: 132–9. 2015.
23. Frankl SN, Shire FR FH. Should the parent remain with the child in the dental operatory? J Dent Child; 29(2):150–63.1962.
24. Rubido S, Fernández-Feijoo J, Limeres J, García-Caballero L, Abeleira MT, Diz P. In vivo antiplaque effect of three edible toothpastes. Med Oral Patol Oral Cir Bucal; 19(1): e88-e92. 2014,
25. Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods; 39(2):175–91. 2007.
26. Hamada S, Iritani S, Miyake T, inventors; Hayashibara Seibutsu Kagaku Kenkyujo KK, assignee. Purified propolis-extract, and its preparation and uses. United States patent US 5,529,779. 1996.
27. Imfeld T. Chewing gum–Facts and fiction: Chewing gum- facts and fiction:a review of gum-chewing and oral health. Crit Rev Oral Biol Med; 10(3):405–19.1999.
28. Lakshmi SV, Yadav HK, Mahesh Kp, Uniyal S, Ayaz A, NagavarmaBVN. Formulation and evaluation of medicated chewing gum as antiplaque and antibacterial agent. J Young Pharm; 6(4):3–10. 2014.
29. Al-Hasani M, Hanno A, Dowidar K, Mostafa O, Soliman S. Effectiveness of Egyptian Propolis on Dental Plaque Formation in High Caries. Alexandria Dent J; 41(2):194–8. 2016.
30. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal;6(2):71–9. 2016.
31. Denbesten P, Hee M, Ko S. Fluoride levels in whole saliva of preschool children after brushing with 0.25 g (pea-sized) as compared 1.0 g (fullbrush) of a fluoriiIe dentifrice. Pediatr Dent ;18(4):277–80. 1996.
32. Imfeld T. Chlorhexidine-containing chewing gum. Clinical documentation. Schweiz Monatsschr Zahnmed; 116(5):476–83. 2006.
33. Syed M, Chopra R, Shrivastava V, Sachdev V. Comparative evaluation of 0.2% chlorhexidine mouthwash, xylitol chewing gum, and combination of 0.2% chlorhexidine mouthwash and xylitol chewing gum on salivary streptococcus mutans and biofilm Levels in 8- to 12-year-old children. Int J Clin Pediatr Dent; 9 (4):313–9. 2016.
34. O’Leary TJ, Drake RB NaylorJE. the plaque control record. J Periodontol ;43(1):38. 1972.
35. Yang Qiong X, Zhang Q, Lu Ying L, Yang R, Liu Y, Zou J. Genotypic distribution of candida albicans in dental biofilm of chinese children associated with severe early childhood caries. Arch Oral Biol; 57(8):1048–53. 2012.
36. Elnakady YA, Rushdi AI, Franke R, Abutaha N, Ebaid H, Baabbad M, et al. Characteristics, chemical compositions and biological activities of propolis from Al-Bahah, Saudi Arabia. Sci Rep; 7: 41453 . 2017.
37. de Carvalho FG, Silva DS, Hebling J, Spolidorio LC, Spolidorio DMP. Presence of mutans streptococci and candida spp. in dental plaque/dentine of carious teeth and early childhood caries. Arch Oral Biol; 51(11):1024–8. 2006.
38. Cline ME, Herman J, Shaw ER, Morton RD. Standardization of the visual analogue scale. Nurs Res; 41(6):378–80. 1992.
39. Sedgwick P. Intention to treat analysis versus per protocol analysis of trial data., BMJ. 2015;6; 350: h681.
40. Duailibe SA, Gonçalves AG, Ahid FJ. Effect of a propolis extract on streptococcus mutans counts in vivo, J. Appl. Oral Sci; 15(5): 420–3. 2007.
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