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Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study
1Pediatric Dentistry, Faculty of Stomatology, Autonomous University of San Luis Potosí, San Luis Potosí, México
2Basic Science Laboratory, Faculty of Stomatology, Autonomous University of San Luis Potosí, San Luis Potosí, México
3Oral Biochemical, Microbiology and Pathology Laboratory, Faculty of Stomatology, Autonomous University of San Luis Potosí, San Luis Potosí, México
DOI: 10.17796/1053-4625-44.5.5 Vol.44,Issue 5,September 2020 pp.315-322
Published: 01 September 2020
*Corresponding Author(s): Luis Octavio Sánchez-Vargas E-mail: octavio.sanchez@uaslp.mx
Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans. Study design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Serial dilutions of 10-1 to 10-7 were made and viability was verified and quantified. For the antagonism tests, a suspension/ inoculum of Lactobacillus strains recovered from each commercial preparation (4-30 x 109 ) and C. albicans ATCC 90028 (1.5-8 x 108 ) was prepared in MRS broth and incubated for 48 hours at 36°C, then plated on Dextrose Sabouraud Agar with Chloramphenicol and Rogosa Agar and the colony-forming units (CFU) were quantified. Additionally, viability was evaluated using the LIVE/DEAD® Yeast and Bacterial Viability kit. Results: The probiotic that produced the highest acidity of the medium was Lactipan®, followed by Probiseis® and Liolactil®, while Probio Hp+® showed the least change. Probiseis® was determined to have the highest growth of probiotic bacteria and the highest inhibition on C. albicans, followed by Lactipan®; Liolactil® and ProbioHp+® showed the least effect. In fluorescence tests, ProBiseis® showed the best effect, followed by Liolactil® and Lactipan®; Probio Hp+® had less of an effect. Conclusions: Two commercial products (ProBiseis and Lactipan) whose formulations have L. acidophilus, L. casei, L. rhamnosus, L. plantarum, B. infantis, and S. thermophilus have a greater inhibitory effect on C. albicans ATCC 90028
Candida albicans; Antagonistic effect; Lactobacillus; Colonization
Liz Mariana Hernández-Bautista,Raúl Márquez-Preciado,Marine Ortiz-Magdaleno,Amaury Pozos-Guillén,Saray Aranda-Romo,Luis Octavio Sánchez-Vargas. Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study. Journal of Clinical Pediatric Dentistry. 2020. 44(5);315-322.
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