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Open AccessCharacterization of Stem Cells from Human Exfoliated Deciduous Anterior Teeth with Varying Levels of Root Resorption
Characterization of Stem Cells from Human Exfoliated Deciduous Anterior Teeth with Varying Levels of Root Resorption
1Department of Pedodontics and Preventive Dentistry, A. B. Shetty Memorial Institute of Dental Sciences, Mangaluru, India
2Nitte University Centre for Stem Cell Research and Regenerative Medicine, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Mangalore, India
3Department of Pedodontics and Preventive Dentistry, A. B. Shetty Memorial Institute of Dental Sciences, Nitte (Deemed to be University), Mangalore, India
*Corresponding Author(s): Amitha M. Hegde E-mail: amipedo9@gmail.com
Abstract
Background: Deciduous teeth undergo the physiologic process of resorption, during which the remnant pulp undergoes activation. However, the quality of stem cells obtained at various stages of root resorption has not been documented. Objective: To isolate and characterize stem cells from deciduous teeth with varying levels of root resorption. Study design: Healthy primary anterior teeth were extracted according to the treatment needs of the patient. The teeth were categorized into SHED(1/3)- teeth with 0 to 1/3rd root resorption, SHED(2/3)- teeth with 1/3rd to 2/3rd root resorption, and SHED(COMP)- teeth with more than 2/3rd root resorption. SHED were characterized based on their morphology, viability, proliferation rate, population doubling time, expression of cell surface markers, and in vitro differentiation potential into osteocytes and adipocytes. Results: SHED from all three groups demonstrated largely similar morphological and cellular characteristics. However, SHED(2/3) showed relatively better characteristics in terms of growth kinetics and phenotypic marker expression. Also, the differentiation ability for osteogenic and adipogenic cell lineages was slightly higher in SHED(1/3) and SHED(2/3) compared with SHED(COMP). Conclusion: Based on the cellular, phenotypic and biological characteristics, it is suggested that SHED (2/3) could be a useful source for tissue regeneration, and warrants further investigations.
Keywords
Stem cells from human exfoliated deciduous teeth; SHED, Root resorption; Cellular properties; Differentiation; In vitro
Cite and Share
Rachaita Chhabra,Shama Rao,B Mohana Kumar,A.Veena Shetty,Amitha M. Hegde,Meghna Bhandary. Characterization of Stem Cells from Human Exfoliated Deciduous Anterior Teeth with Varying Levels of Root Resorption. Journal of Clinical Pediatric Dentistry. 2021. 45(2);104-111.
References
1. Xie F, He J, Chen Y, Hu Z, Qin M, Hui T. Multi-lineage differentiation and clinical application of stem cells from exfoliated deciduous teeth. Hum Cell. 2020;33(2):295-302.
2.Miura M, Gronthos S, Zhao M, et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A. 2003;100(10):5807-5812. doi:10.1073/pnas.0937635100
3. Sloan AJ, Waddington RJ. Dental pulp stem cells: what, where, how?. Int J Paediatr Dent. 2009;19(1):61-70. doi:10.1111/j.1365-263X.2008.00964.x
4. Suchánek J, Visek B, Soukup T, et al. Stem cells from human exfoliated deciduous teeth— isolation, long term cultivation and phenotypical analysis. Acta Medica (Hradec Kralove). 2010;53(2):93-99. doi:10.14712/18059694.2016.66
5. Martacić JD, Francuski J, Luzajić T, et al. Characterization of deciduous teeth stem cells isolated from crown dental pulp. Vojnosanit Pregl. 2014;71(8):735-741.
6. Zhang N, Chen B, Wang W, et al. Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth. Mol Med Rep. 2016;14(1):95-102. doi:10.3892/mmr.2016.5214
7. Hara K, Yamada Y, Nakamura S, Umemura E, Ito K, Ueda M. Potential characteristics of stem cells from human exfoliated deciduous teeth compared with bone marrow-derived mesenchymal stem cells for mineralized tissueforming cell biology. J Endod. 2011;37(12):1647-1652. doi:10.1016/j. joen.2011.08.023
8. Kunimatsu R, Nakajima K, Awada T, et al. Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells. Biochem Biophys Res Commun. 2018;501(1):193-198. doi:10.1016/j.bbrc.2018.04.213
9. Shekar R, Ranganathan K. Phenotypic and growth characterization of human mesenchymal stem cells cultured from permanent and deciduous teeth. Indian J Dent Res. 2012;23(6):838-839. doi:10.4103/0970-9290.111281
10. Wang X, Sha XJ, Li GH, et al. Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. Arch Oral Biol. 2012;57(9):1231- 1240. doi:10.1016/j.archoralbio.2012.02.014.
11. Samiei M, Aghazadeh M, Movassaghpour AA, Fallah A, Aminabadi NA, Pakdel SM, Sakhinia E, Estiar MA, Omidi Y, Pakdel F. Isolation and characterization of dental pulp stem cells from primary and permanent teeth. J Am Sci;9(12). 2013.
12. Vishwanath VR, Nadig RR, Nadig R, Prasanna JS, Karthik J, Pai VS. Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth: An in vitro study. J Conserv Dent. 2013;16(5):423-428. doi:10.4103/0972-0707.117509
13. Taguchi T, Yanagi Y, Yoshimaru K, et al. Regenerative medicine using stem cells from human exfoliated deciduous teeth (SHED): a promising new treatment in pediatric surgery. Surg Today. 2019;49(4):316-322. doi:10.1007/s00595-019-01783-z
14. Yildirim S, Yapar M, Sermet U, Sener K, Kubar A. The role of dental pulp cells in resorption of deciduous teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105(1):113-120. doi:10.1016/j.tripleo.2007.06.026
15. Bernardi L, Luisi SB, Fernandes R, et al. The isolation of stem cells from human deciduous teeth pulp is related to the physiological process of resorption. J Endod. 2011;37(7):973- 979. doi:10.1016/j.joen.2011.04.010
16. Nakajima K, Kunimatsu R, Ando K, et al. Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth. Sci Rep. 2019;9(1):16764. Published 2019 Nov 14. doi:10.1038/ s41598-019-53265-4
17. Rosa V, Dubey N, Islam I, Min KS, Nör JE. Pluripotency of Stem Cells from Human Exfoliated Deciduous Teeth for Tissue Engineering. Stem Cells Int. 2016;2016:5957806. doi:10.1155/2016/5957806
18. Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317. doi:10.1080/14653240600855905
19. Nakamura S, Yamada Y, Katagiri W, Sugito T, Ito K, Ueda M. Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp. J Endod. 2009;35(11):1536-1542. doi:10.1016/j. joen.2009.07.024
20. Sivasankar V, Ranganathan K. Growth characteristics and expression of CD73 and CD146 in cells cultured from dental pulp. J Investig Clin Dent. 2016;7(3):278-285. doi:10.1111/jicd.12155
21. Boxall SA, Jones E. Markers for characterization of bone marrow multipotential stromal cells. Stem Cells Int. 2012;2012:975871. doi:10.1155/2012/975871
22. Tsai CL, Chuang PC, Kuo HK, Chen YH, Su WH, Wu PC. Differentiation of Stem Cells From Human Exfoliated Deciduous Teeth Toward a Phenotype of Corneal Epithelium In Vitro. Cornea. 2015;34(11):1471-1477. doi:10.1097/ICO.0000000000000532
23. Tsagias N, Koliakos KK, Spyridopoulos T, et al. A simple method forthe quantitation of the stem cells derived from hu man exfoliated deciduous teeth using a luminescent cell viability assay. Cell Tissue Bank. 2014;15(3):491-499. doi:10.1007/s10561-013-9419-4
24. Yu S, Diao S, Wang J, Ding G, Yang D, Fan Z. Comparative analysis of proliferation and differentiation potentials of stem cells from inflamed pulp of deciduous teeth and stem cells from exfoliated deciduous teeth. Biomed Res Int. 2014;2014:930907. doi:10.1155/2014/930907
25. Yamaza T, Kentaro A, Chen C, et al. Immunomodulatory properties of stem cells from human exfoliated deciduous teeth. Stem Cell Res Ther. 2010;1(1):5. Published 2010 Mar 15. doi:10.1186/scrt5
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