Evaluation of Aloevera Gel as a Storage Medium in Maintaining the Viability of Periodontal Ligament Cells - An in Vitro Study

Objective: To investigate the effectiveness of aloevera gel as a new storage medium in maintaining the viability of periodontal ligament cells. Study design: Premolars extracted for orthodontic reason were obtained. Confluent monolayers of fibroblasts were grown by cell culture method from the PDL cells isolated from the extracted teeth. One ml of this cell suspension was transferred to wells of culture plates, incubated for 24 hrs, followed by exposure to the three experimental media, Hank's balanced salt solution (HBSS), aloevera gel, and packaged drinking water. These plates were then assessed for viable cells using trypan blue dye exclusion test with haemocytometer after 15, 30, 60, 90 and 120 mins. The results obtained were statistically analysed using one-way analysis of variance (ANOVA). Results: At 15 min, HBSS presented maximum mean percentage of viable PDL cells (89%), followed by aloevera at 81% and packaged drinking water at 10%. Aloevera demonstrated 71%, 59%, 57% viable cells at 30, 60, 90 mins respectively. At 120 min, HBSS presented 57% viable cells followed by aloevera gel (45%) and packaged drinking water (3%). No statistical significant difference was observed between HBSS and aloevera gel. Conclusions: Within the parameters of this study, both aloevera gel and HBSS were effective in maintaining the viability of PDL cells. Hence, aloevera gel could be used as a storage media for avulsed tooth in situations where availability of HBSS is in question.

PDL viability, storage media, cell culture, HBSS, Aloevera gel

1. Andreasen F.M., Daugaard Jensen l. Treatment of traumatic dental injuries in children. Current Opinion in Dentistry, 1:535-46,1991.

2. Saroglu I, Sonmez H. The prevalence of traumatic injuries treated in the pedodontic clinic of Ankara University, Turkey, during 18 months. Dent Traumatol 18:299-303, 2002.

3. Thomas T, Gopikrishna V, Kandaswamy D. Comparative evaluation of maintenance of cell viability of an experimental transport media “coconut water” with Hank’s balanced salt solution and milk, for transportation of an avulsed tooth: An in vitro cell culture study. j Conserv Dent. 11(1):22-9, 2008.

4. Mendonça-Filho RR. Bioactive Phytocompounds:New Approaches in the Phytosciences. In: Modern Phytomedicine Turning Medicinal Plants into Drugs. 1st ed. ed. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co.,2006.

5. Hamman JH. Composition and applications of Aloe vera leaf gel. Mole-cules, 13(8):1599-616, 2008.

6. Ragnarsson B, Carr G, Daniel JC. Isolation and growth of human peri-odontal ligament cells in vitro. J Dent Res. 64(8):1026-30, 1985.

7. Er K, Polat ZA, Ozan F, Tasdemir T, Sezer U, Siso SH. Cytotoxicity anal-ysis of strontium ranelate on cultured human periodontal ligament fibro-blasts: a preliminary report. J Formos Med Assoc. 107(8):609-15, 2008.

8. Ashkenazi M, Sarnat H, Keila S In vitro viability mitogenicity and clono-genic capacity of periodontal ligament cells after storage in six different media. Endod Dent Traumatol, 15:149-56, 1999.

9. Doyle DL, Dumsha TC, Sydiskis RJ. Effect of soaking in Hank’s balanced salt solution or milk on PDL cell viability of dry stored human teeth. Endod Dent Traumatol, 14:221 - 4, 1998.

10. Ashkenazi M, Marouni M, Sarnat H In vitro viability, mitogenicity and clonogenic capacity of periodontal ligament cells after storage in four media at room temperature. Endod Dent Traumatol, 16:63-70, 2000.

11. Marino TG, LA W. Determination of periodontal ligament cell viability in long shelf-life milk. Journal of Endodontics, 26:699 - 702, 2000.

12. Krasner P, Person P. Preserving avulsed teeth for replantation. J Am Dent Assoc. Nov 123(11):80-8, 1992.

13. Ozan F, Polat ZA, Er K, Ozan U, Deger O. Effect of propolis on survival of periodontal ligament cells: new storage media for avulsed teeth. J Endod. May 33(5):570-3, 2007.

14. Hiltz T. Vitality of human lip fibroblasts in milk, Hank’s balanced salt solu-tion and ViaSpan storage media. Endod Dent Traumatol, 7: 69 -72, 1991.

15. Trope M. Clinical management of the avulsed tooth. Dent Clin North Am. Jan 39(1):93-112, 1995.

16. Martin, Pileggi. A quantitative analysis of Propolis: a promising new storage media following avulsion. Dent Traumatol, 20:85 -9, 2004.

17. Gopikrishna V, Baweja PS, Venkateshbabu N, Thomas T, Kandaswamy D. Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival. J Endod. 34(5):587-9, 2008.

18. Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabi nia N, et al. A new storage medium for an avulsed tooth. J Contemp Dent Pract, 9(6):25-32, 2008.

19. Khare C.P, editor. Indian Medicinal Plants : An Illustrated Dictionary. Verlag Berlin/Heidelberg: springer; 2007.

20. Anonymous. Oral ulcers remedy gets FDA clearance. J Am Dent Assoc 125(10):1308, 10, 1994.

21. Choonhakarn C, Busaracome P, Sripanidkulchai B, Sarakarn P. The effi-cacy of aloe vera gel in the treatment of oral lichen planus: a randomized controlled trial. Br J Dermatol 158(3):573-7, 2008.

22. Kaim JM, Gultz J, Do L, Scherer W. J. An in vitro investigation of the antimicrobial activity of an herbal mouthrinse. J Clin Dent 9(2):46-8, 1998.

23. George D, Bhat SS, Antony B. Comparative evaluation of the antimicrobial efficacy of aloe vera tooth gel and two popular commercial toothpastes: an in vitro study. Gen Dent, 57(3):238-41, 2009.

24. Soder PO, Otteskog P, Andreasen JO, Moder T. Effect of drying on viability of periodontal membrane. Scand J Dent Res, 85:164-8, 1977.

25. Buttke TM, Trope M Effect of catalase supplementation in storage media for avulsed teeth. Dent Traumatol, 19:103-8, 2003.

26. Lindskog S, Blomlof L. Influence of osmolality and composition of some storage media on human periodontal ligament cells. Acta Odontol Scand, 40(6):435-41, 1982.