In Vitro Evaluation of Microleakage and Microhardness of Ethanolic Extracts of Propolis in Different Proportions Added to Glass Ionomer Cement

Objective: To evaluate the effect of ethanolic extracts of propolis (EEP) addition in different proportions to glass ionomer cement (GIC) on microleakage and microhardness of GIC. Study design: The cement was divided into four groups: one using the original composition and three with 10%, 25%, and 50% EEP added to the liquid and then manipulated. For microleakage assessment, sixty primary molars were randomly divided into four groups (n=15). Standard Class II cavities were prepared and then filled with EEP in different proportions added to GICs. Microleakage test was performed using a dye penetration method. The data were analyzed using one-way ANOVA and Mann - Whitney U tests (α = 0.05). Disc shaped specimens were prepared from the tested GIC to determine Vickers hardness (VHN). The data were analyzed using one-way ANOVA and post hoc Tukey test (α = 0.05). Results: There were no statistically significant differences between the groups in terms of microleakage (p > 0.05). There were statistically significant differences between the VHN values of groups (p < 0.05). Increasing addition of EEP to GIC statistically significantly increased VHN value of GIC (p < 0.05). Conclusions: The addition of EEP to GIC increased the microhardness of the GIC and did not adversely affect the microleakage. Thus, it might be used during routine dental practice due to its antibacterial properties

Ethanolic extracts of propolis, glass ionomer cement, microhardness microleakage

1. Mahapoka E, Arirachakaran P, Watthanaphanit A, Rujiravanit R, Poolthong S. Chitosan whiskers from shrimp shells incorporated into dimethacry-late-based dental resin sealant. Dent Mater J. 2012; 31: 273-9.

2. Dziedzic A, Kubina R, Wojtyczka RD, Kabala-Dzik A, Tanasiewicz M, Morawiec T. The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva. Evid Based Complement Alternat Med. 2013; 2013: 681891.

3. Frencken JE, Pilot T, Songpaisan Y, Phantumvanit P. Atraumatic restorative treatment (ART): rationale, technique, and development. J Public Health Dent. 1996; 56: 135-40; discussion 61-3.

4. Wang L, Lopes LG, Bresciani E, Lauris JR, Mondelli RF, Navarro MF. Evaluation of Class I ART restorations in Brazilian schoolchildren: three-year results. Spec Care Dentist. 2004; 24: 28-33.

5. Maruo IT, Godoy-Bezerra J, Saga AY, Tanaka OM, Maruo H, Camargo ES. Effect of etching and light-curing time on the shear bond strength of a resin-modified glass ionomer cement. Braz Dent J. 2010; 21: 533-7.

6. Flores AR, Saez EG, Barcelo F. Metallic bracket to enamel bonding with a photopolymerizable resin-reinforced glass ionomer. Am J Orthod Dentofa-cial Orthop. 1999; 116: 514-7.

7. Can-Karabulut DC, Batmaz I, Solak H, Tastekin M. Linear regression modeling to compare fluoride release profiles of various restorative mate-rials. Dent Mater. 2007; 23: 1057-65.

8. Hatunoglu E, Ozturk F, Bilenler T, Aksakalli S, Simsek N. Antibacterial and mechanical properties of propolis added to glass ionomer cement. Angle Orthod. 2014; 84: 368-73.

9. Topcuoglu N, Ozan F, Ozyurt M, Kulekci G. In vitro antibacterial effects of glass-ionomer cement containing ethanolic extract of propolis on Strepto-coccus mutans. Eur J Dent. 2012; 6: 428-33.

10. Troca VB, Fernandes KB, Terrile AE, Marcucci MC, Andrade FB, Wang L. Effect of green propolis addition to physical mechanical properties of glass ionomer cements. J Appl Oral Sci. 2011; 19: 100-5.

11. Prabhakar AR, Prahlad D, Kumar SR. Antibacterial activity, fluoride release, and physical properties of an antibiotic-modified glass ionomer cement. Pediatr Dent. 2013; 35: 411-5.

12. Klai S, Altenburger M, Spitzmuller B, Anderson A, Hellwig E, Al-Ahmad A. Antimicrobial effects of dental luting glass ionomer cements on Strepto-coccus mutans. ScientificWorldJournal. 2014; 2014: 807086.

13. Marti LM, Mata M, Ferraz-Santos B, Azevedo ER, Giro EM, Zuanon AC. Addition of chlorhexidine gluconate to a glass ionomer cement: a study on mechanical, physical and antibacterial properties. Braz Dent J. 2014; 25: 33- 7.

14. Ahangari Z, Naseri M, Jalili M, Mansouri Y, Mashhadiabbas F, Torkaman A. Effect of propolis on dentin regeneration and the potential role of dental pulp stem cell in Guinea pigs. Cell J. 2012; 13: 223-8.

15. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol. 1999; 64: 235-40.

16. Hayacibara MF, Koo H, Rosalen PL et al. In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. J Ethnophar-macol. 2005; 101: 110-5.

17. M R, Sajjan GS, B NK, Mittal N. Effect of different placement techniques on marginal microleakage of deep class-II cavities restored with two composite resin formulations. J Conserv Dent. 2010; 13: 9-15.

18. Silverman E, Cohen M, Demke RS, Silverman M. A new light-cured glass ionomer cement that bonds brackets to teeth without etching in the presence of saliva. Am J Orthod Dentofacial Orthop. 1995; 108: 231-6.

19. Takahashi Y, Imazato S, Kaneshiro AV, Ebisu S, Frencken JE, Tay FR. Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach. Dent Mater. 2006; 22:

647- 52.

20. Mickenautsch S, Mount G, Yengopal V. Therapeutic effect of glass-iono-mers: an overview of evidence. Aust Dent J. 2011; 56: 10-5; quiz 103.

21. Mazzaoui SA, Burrow MF, Tyas MJ. Fluoride release from glass ionomer cements and resin composites coated with a dentin adhesive. Dent Mater. 2000; 16: 166-71.

22. Hu J, Du X, Huang C, Fu D, Ouyang X, Wang Y. Antibacterial and physical properties of EGCG-containing glass ionomer cements. J Dent. 2013; 41: 927- 34.

23. Nicholson JW. Chemistry of glass-ionomer cements: a review. Biomate-rials. 1998; 19: 485-94.

24. Shiozawa M, Takahashi H, Iwasaki N, Uo M. Effect of calcium chloride solution immersion on surface hardness of restorative glass ionomer cements. Dent Mater J. 2013; 32: 828-33.

25. Taylor MJ, Lynch E. Microleakage. J Dent. 1992; 20: 3-10.

26. Tuzuner T, Ulusu T. Effect of antibacterial agents on the surface hardness of a conventional glass-ionomer cement. J Appl Oral Sci. 2012; 20: 45-9.

27. Moshaverinia A, Brantley WA, Chee WW et al. Measure of microhard-ness, fracture toughness and flexural strength of N-vinylcaprolactam (NVC)-containing glass-ionomer dental cements. Dent Mater. 2010; 26: 1137-43.

28. Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: fruits. J Agric Food Chem. 2001; 49: 5315-21.

29. Tipoe GL, Leung TM, Hung MW, Fung ML. Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection. Cardiovasc Hematol Disord Drug Targets. 2007; 7: 135-44.

30. Lo CY, Hsiao WT, Chen XY. Efficiency of trapping methylglyoxal by phenols and phenolic acids. J Food Sci. 2011; 76: H90-6.

31. Prentice LH, Tyas MJ, Burrow MF. The effect of ytterbium fluoride and barium sulphate nanoparticles on the reactivity and strength of a glass-ion-omer cement. Dent Mater. 2006; 22: 746-51.

32. Zanata RL, Magalhaes AC, Lauris JR, Atta MT, Wang L, Navarro MF. Microhardness and chemical analysis of high-viscous glass-ionomer cement after 10 years of clinical service as ART restorations. J Dent. 2011; 39: 834-40.