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Original Research

Open Access

Association between S. mutans and S. sanguinis in Severe Early childhood Caries and Caries-Free Children A Quantitative Real-Time PCR Analysis

  • Kemthong Mitrakul1,*,
  • Kutkao Vongsawan1
  • Assavinee Sriutai1
  • Wipaphan Thosathan1

1From the Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol, Bangkok, Thailand.

DOI: 10.17796/1053-4628-40.4.281 Vol.40,Issue 4,July 2016 pp.281-289

Published: 01 July 2016

*Corresponding Author(s): Kemthong Mitrakul E-mail: mkemthong@yahoo.com

Abstract

To identify S. mutans and S. sanguinis in initial and overnight plaque between 2 groups and to analyze the association between them and caries-related factors. Study design: Collected supra gingival plaque from 140 Thai children aged 2-6 years old (S-ECC = 68, caries-free=72). Recorded plaque and gingival indices, dmft score, salivary mutans streptococci level, pH and buffer capacity. Firstly, the overnight plaque was collected, then, 4 hrs. after a thorough prophylaxis, the initial plaque was collected. Accessed parent’s attitude and behavior in children’s oral hygiene care and diet practice using a questionnaire. A quantitative real-time PCR was performed. Results: For initial plaque, S. sanguinis was higher in cariesfree. S. mutans (0.011) and S. mutans/S. sanguinis ratio (0.005) were higher in S-ECC. S. sanguinis amount was inverse correlated with dmft (0.00), gingival index (0.044), and plaque index (0.011). For overnight plaque, S. mutans (0.00) and S. mutans/S. sanguinis ratio (0.005) were also higher in S-ECC. S. mutans, S. mutans/S. sanguinis ratio were positive correlated to dmft (0.00). Parent education levels (0.004) and bottle feeding (0.011) between 2 groups were different. Conclusion: S. sanguinis, S. mutans and their ratio in initial and overnight plaque, low family income and bottle feeding are strongly associated with S-ECC.

Keywords

dental plaque, Streptococcus mutans, Streptococcus sanguinis, severe early childhood caries, PCR

Cite and Share

Kemthong Mitrakul, Kutkao Vongsawan,Assavinee Sriutai,Wipaphan Thosathan. Association between S. mutans and S. sanguinis in Severe Early childhood Caries and Caries-Free Children A Quantitative Real-Time PCR Analysis. Journal of Clinical Pediatric Dentistry. 2016. 40(4);281-289.

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