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Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth
1Facultad de Estomatología, Universidad Autónoma de San Luis Potosí. Av. Dr. Manuel Nava #2, Zona Universitaria, CP 78290, San Luis Potosí, SLP, México.
*Corresponding Author(s): Amaury Pozos-Guillén E-mail: apozos@uaslp.mx
The aim of the study was to determine the eugenol concentrations at which toxicity occurs in human dental pulp fibroblasts of primary teeth. Study design: Samples of primary dental pulp tissue were taken. Tissue samples were seeded by means of explant technique and used in the 4th-5th pass. Single Cell Gel Electrophoresis (Comet), phenazine MeThoSulfate (MTS), LIVE/DEAD® Cell Viability/Toxicity and trypan blue assays for evaluation of the cytotoxicity of increasing concentrations of eugenol (0.06 to 810 μM) were performed. Results: The results of toxicity tests showed toxic effects on dental pulp fibroblasts, even at very low concentrations of eugenol (0.06 μM). Very low concentrations of eugenol produce high toxicity in human dental pulp fibroblasts. Conclusions: All of the concentrations of eugenol that we evaluated produced high toxicity in human dental pulp fibroblasts of primary teeth.
Toxicity, eugenol, fibroblasts.
Maria Escobar-García,Karen Rodríguez-Contreras,Socorro Ruiz-Rodríguez,Mauricio Pierdant-Pérez,Bernardino Cerda-Cristerna,Amaury Pozos-Guillén. Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth. Journal of Clinical Pediatric Dentistry. 2016. 40(4);312-318.
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