Title
Author
DOI
Article Type
Special Issue
Volume
Issue
Comparative Quantitative Assessments of Salivary Ion Activity Product for Hydroxyapatite and Buffering Capacity in Children with Different Caries Experience
1Department of Pedodontics and Preventive Dentistry, K. D. Dental College and Hospital, Mathura, India
2Department of Pedodontics and Preventive Dentistry, Treethankar Dental College and Hospital, Moradabaad, India.
DOI: 10.17796/1053-4628-40.6.480 Vol.40,Issue 6,November 2016 pp.480-485
Published: 01 November 2016
*Corresponding Author(s): Siddharth Anand E-mail: drsiddharthanand@yahoo.co.in
If a relation exists between salivary IPHA, buffer capacity and caries experience, then this relationship could be used as screening chair side test for caries risk assessment. Study design: One hundred ninety seven children aged 4 to 6 years were examined. Data was collected by interview and clinical examination. They were divided into low, moderate and high caries experience group of 20 children each. Two ml of each sample was used to measure the pH value with pH meter. Regarding the buffering capacity, freshly prepared hydrochloric acid (HCl) was titrated into saliva and pH was recorded. The collected saliva samples were sent to Laboratory for measurement of calcium and phosphorus. IPHA was calculated and the negative logarithms of IPHA were used to determine the enamel solubility. The correlation between salivary IPHA, buffering capacity and caries experience were evaluated. Results: There was a significant relation between pH, log IPHA and dental caries experience, it could be considered as a predictor of dental caries. Conclusion: pH measurement after HCl titration in saliva could be used as chair side screening test for the assessment of caries risk.
ion activity product for hydroxyapatite (IPHA), buffering capacity and hand held pH meter
Siddharth Anand,Updesh Masih,Ramakrishna Yeluri. Comparative Quantitative Assessments of Salivary Ion Activity Product for Hydroxyapatite and Buffering Capacity in Children with Different Caries Experience. Journal of Clinical Pediatric Dentistry. 2016. 40(6);480-485.
1. Vahedi M, Davoodi P, Goodarzi MT, Rezaei Soufi L, Jazaeri M, Mortazavi H, Moghimbeigi E. Comparison of salivary ion activity product for hydroxyapatite (IPHA), alkaline phosphatase and buffering capacity of adults according to age and caries severity. J Dent Shiraz Univ Med Sci;13(4):139-145. 2012.
2. Farsi N, Amoudi NA, Farsi J, Bokhary S, Sonbul H. Periodontal health and its relationship with salivary factors among different age groups in a Saudi population. Oral Health Prev Dent;6:147-154. 2008.
3. Lenander-Lumikari M, Loimaranta V. Saliva and dental caries. Adv Dent Res;14:40-47. 2000.
4. Roberson Tm, Heymann HO, Swift EJ. Art and science of operative dentistry. 5th ed., St Louis:Mosby co;2006. Pg 67.
5. Birkhed D, Heintze U. Salivary secretion rate, buffer capacity and pH. In Human saliva: clinical chemistry and microbiology. Tenovuo J editor 1989, Vol 1 Boca Raton, FL:CRC Press, pg 25-73.
6. Ericsson Y. Clinical investigations of the salivary buffering action. Adv Odontol Scand;17:131-165. 1959.
7. Heintze U, Birkhed D, Bjorn H. Secretion rate and the buffer effect of resting and stimulated whole saliva as a function of age and sex. Swed Dent J;7: 227-238. 1983.
8. Pinkham J, Casmassimo P, Mc Tigue D, Fields H, Nowak A. Pediatric Dentistry Infancy Through Adolescence; 4th ed., China: Elsevier Inc 2005: pg. 199-204.
9. Shahrabi M, Nikfarjan J, Alikhani A, Akhoundi N, Ashtiani M, Seraj B. A comparison of salivary calcium, phosphate and alkaline phosphatase in children with severe, moderate caries and caries free in Tehran’s kindergartens. J Ind Soc Pedod Prev Dent; 26: 74-77. 2008.
10. Hay DI, Schluckebier SK, Moreno EC. Equilibrium dialysis and ultrafiltration studies of calcium and phosphate binding by human salivary proteins. Implicatons for salivary supersaturtion with respect to calcium phosphate salts. Calcif Tissue Int; 34: 531-538. 1982.
11. Lagerlof F. Effects of flow rate and pH on calcium phosphate saturation in human parotid saliva. Caries Res; 17: 403-411. 1983.
12. Harada M, Udagawa N, Fukasawa K, Hiraoka BY, Mogim M. Inorganic pyrophosphatase activity of purified bovine pulp alkaline phosphatase at physiological pH. J Dent Res; 65: 125-127. 1986.
13. Margolis HC, Zhang YP, Lee CY, Kent RL Jr., Morneo EC. Kinetics of enamel demineralization in vitro. J Dent Res; 78: 1326-1335. 1989.
14. Shellis RP, Wilsoon RM. Apparent solubility distributions of hydroxyapatite and enamel apatite. J Colloid Interface Sci; 278: 325-332. 2004.
15. Patel MV, Fox JL, Higuchi WI. Effect of acid type on kinetics and mechanism of dental enamel demineralization. J Dent Res; 66: 1425-1430. 1987.
16. Aiuchi H, Kitasako Y, Fukuda Y, Nakashima S, Burrow MF, Tagami J. Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential. Aust Dent J; 53: 167-171. 2008.
17. Gandhy M, Damle SG. Relation of salivary inorganic phosphorous and alkaline phosphatase to the dental caries status in children. J Ind Soc Pedod Prev Dent; 21: 135-138. 2003.
18. Chang HS, Laurence J, Terrence J. The effect of orthodontic treatment on salivary flow, pH, buffer capacity, and levels of mutans streptococci and lactobacilli. Aust Orthod J; 15: 229-234. 1999.
19. Kitasako Y, Moritsuka M, Foxton RM, Ikeda M, Tagami J, Nomura S. Simplified and quantitative saliva buffer capacity test using a hand held ph meter. Am J Dent; 18: 147-150. 2005.
20. Moritsuka M, Kitasako Y, Burrow MF, Ikeda M, Tagami J. The pH change after HCL titration into resting and stimulated saliva for a buffering capacity test. Aust Dent J; 51: 170-174. 2006.
21. Bhayat A, Vergotine G, Yengopal V, Rudulph MJ. The impact of service learning on two groups of South African dental students. J Dent Edu; 75: 1482-1488. 2011.
22. Karshan M. Factors in saliva correlated with dental caries. J Dent Res; 18(5): 395-407. 1939.
23. Wiktorsson AM, Martinsson T, Zimmerman M. Salivary levels of lactobacilli, buffer capacity and salivary flow rate related to caries activity among adults in communities with optimal and low water fluoride concentrations. Swed Dent J; 16: 231-237. 1992.
24. Powell LV, Leroux BG, Persson RE, Kiyak HA. Factors associated with caries incidence in an elderly population. Comm Dent Oral Epidemiol; 26: 170-176. 1998.
25. Varma S, Banerjee A, Bartlett D. An in vivo investigation of associations between saliva properties, caries prevalence and potential lesion activity in an adult UK population. J Dent; 9: 16-23. 2008.
26. Fenoll-Palomares C, Munoz-Montagud JV, Sanchiz V, Herreros B, Hernandez V, Minguez M, Benages A. Unstimulated salivary flow rate, pH and buffer capacity of saliva in healthy volunteers. Rev Esp Enferm Dig; 96: 773-783. 2004.
27. Miura H, Isogai E, Hirose K, Wakizaka H, Ueda I, Ito N. Application of a sucrose indicator strip to evaluate salivary sucrose clearance. J Dent; 19: 189-191. 1991.
28. Monezgo MC, Tenovuo J, Haunsen H. Dental caries in relation to diet, saliva and cariogenic microorganisms in Tanzanians of selected age groups. Comm Dent Oral Epidemiol; 24: 169-174. 1996.
29. Gabris K, Nagy G, Madlena M, Denes Z, Marton S, Keszthelyi G. Banoczy J. Associations between microbiological and salivary caries activity tests and caries experience in Hungarian adolescents. Caries Res; 33(3): 191-195. 1999.
30. Ruiz Miravet A, Montiel Company JM, Almerich Silla JM. Evaluation of caries risk in a young adult population. Med Oral Patol Oral Cir Bucal; 12: 412-418. 2007.
Top