Degradation of Resin Restorative Materials by Streptococcus Mutans: A Pilot Study

Objective: To evaluate the degradation of three resin based restorative materials by S Mutans. Study design: Class I cavity was prepared in extracted premolars and were randomly divided into 3 groups (Group I – Conventional composite (CC), Group II – Resin Modified GIC and Group III–Giomer). Teeth were then restored by respective restorative material and equally divided in two subgroups (Control and Experimental). Experiment subgroup samples were then incubated in 2 ml of BHI with 1:10 dilution of SM (MTCC-497) grown overnight in BHI whereas control subgroup samples were incubated in BHI without SM. The incubation solution was collected at 2,14 and 30 days interval, and the analysis for identification and quantification of Bis-HPPP was done by High performance Liquid Chromatography. Results: Statistical analysis of the collected data revealed a statistically increased Bis HPPP production in the presence of SM in all the tested materials, with minimum in Resin Modified GIC and a maximum in Conventional Composite (CC). Conclusion: SM degrades the resin based restorative materials & among the tested materials Resin Modified GIC appears to be most Biostable.

Resin, S mutans, degradation, High performance liquid chromatography

1. Jaffer F , Finer Y, and Santerre JP. Interactions between resin monomers and commercial composite resins with human saliva derived esterases. Biomaterials, 23(7):1707-19, 2002.

2. Khalichi P, Singh J, Cvitkovitch DG, Santerre JP. The influence of trieth-ylene glycol derived from dental composite resins on the regulation of Streptococcus mutans gene expression. Biomaterials, 30(4):452-459, 2009.

3. Murray PE, Windsor LJ, Smyth TW, Hafez AA, Cox CF. Analysis of pulpal reactions to restorative procedures, materials, pulp capping, and future therapies. Crit Rev Oral Biol Med. 13(6):509-520, 2002.

4. Burke F, Wilson N, Cheung S, Mjor AI. Influence of patient factors on age of restorations at failure and reasons for their placement and replacement. J Dent, 29(5):317-324,2001.

5. Mjor IA, Dahl JE, Moorhead JE. Age of restorations at replacement in permanent teeth in general dental practice. ActaOdontolScand, 58(3):97-101, 2000.

6. Santerre, J. P., L. Shajii, and B. W. Leung. Relation of dental composite formulations to their degradation and the release of hydrolyzed polymer-ic-resin-derived products. Crit Rev Oral Biol Med, 12(2):136-151, 2001.

7. Finer Y, Santerre JP. The influence of resin chemistry on a dental composite biodegradation. J Biomed Mater Res, 69A:233-246, 2004.

8. Msitra S. Curing reactions of glass-ionomer materials. In: Hunt PR, ed. Glass-ionomers: The next generation. Proceedings of the 2nd Interna-tional Symposium on Glass ionomers. Philadelphia, 13-23, 1994.

9. Kermanshahi S, Santerre JP, Cvitkovitch DG, Finer Y. Biodegradation of resin-dentin interfaces increases bacterial microleakage. J Dent Res, 89(9):996-01, 2010.

10. Brannstrom M. Communication between the Oral Cavity and the Dental-Pulp Associated with Restorative Treatment. Oper Dent, 9(2):57-68, 1984.

11. Bourbia M, Ma D, Cvitkovitch DG, Santerre JP, Finer Y. Cariogenic bacteria degrade dental resin composites and adhesives. J Dent Res, 92:989-994, 2013.

12. Shokati B, Tam LE, Santerre JP, Finer Y. Effect of salivary esterase on the integrity and fracture toughness of the dentin-resin interface. J Biomed Mater Res B Appl Biomater, 94(1):230-7, 2010.

13. Zhu H, Liu M, Sumby P, Lei B. The Secreted Esterase of Group A Strep-tococcus Is Important for Invasive Skin Infection and Dissemination in Mice. Infect Immun, 77(12):5225-32, 2009.

14. Borges MA, Matos IC, Mendes LC, Gomes AS, Miranda MS. Degrada-tion of polymeric restorative materials subjected to a high caries chal-lenge. Dent Mater, 27(3):244-52, 2011.

15. Fucio SB, Carvalho FG, Sobrinho LC, Sinhoreti MA, Puppin-Rontani RM . The influence of 30-day-old Streptococcus mutans biofilm on the surface of esthetic restorative materials–An in vitro study. J Dent, 36(10):833-9, 2008.

16. Gregson KS, Shih H, Gregory RL (2012). The impact of three strains of oral bacteria on the surface and mechanical properties of a dental resin material. Clin Oral Investig, 16(4):1095-103, 2012.