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Original Research

Open Access

Comparison of the Antimicrobial Efficacy of the EndoVac System and Conventional Needle Irrigation in Primary Molar Root Canals

  • Burak Buldur1,*,
  • Arife Kapdan1

1Department of Pediatric Dentistry, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey

DOI: 10.17796/1053-4628-41.4.284 Vol.41,Issue 4,July 2017 pp.284-288

Published: 01 July 2017

*Corresponding Author(s): Burak Buldur E-mail: bbuldur@gmail.com

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Abstract

Purpose: The purpose of this study was to compare the antimicrobial efficacy of the EndoVac system and conventional needle irrigation to eliminate E faecalis in primary molar root canals. Study Design: 60 extracted human primary second molar roots were instrumented up to an apical size .04/35 and randomly divided into two groups; Group 1: conventional needle (n=30) and Group 2: EndoVac (n=30), and four subgroups (two experimental subgroups; (a) 2.5% sodium hypochlorite (NaOCl) + ethylenediaminetetraacetic acid (EDTA) (n=20), (b) ozonated water (OW) + EDTA (n=20), and control groups (c) 5.25% NaOCl (n=10) and (d) saline (n=10). All roots were sterilized and then inoculated with E.faecalis. Before and after final irrigation procedures, root canals were sampled and the grown colony forming units (CFUs) were counted. Data were analyzed by Kruskall-Wallis and Mann-Whitney U tests using a 0.05 significance level. Results: The EndoVac reduced more bacteria than the conventional needle did but it was not statistically significant (p>0.05). NaOCl alone or followed by EDTA totally eliminated bacteria. OW + EDTA showed higher reduction of bacteria but could not totally eliminate bacterias. Conclusions: In the context of bacterial elimination, the EndoVac was not significantly better than the conventional needle. Although, there were fewer CFU/mg when using EndoVac, there was not any statistically significant superiority to conventional needle irrigation. An OW+EDTA regimen showed antibacterial effect in the primary molar root canals but it was significantly less effective than NaOCl+EDTA.

Keywords

antimicrobial, EndoVac, ozonated water, primary teeth, pulpectomy, root canal

Cite and Share

Burak Buldur, Arife Kapdan. Comparison of the Antimicrobial Efficacy of the EndoVac System and Conventional Needle Irrigation in Primary Molar Root Canals. Journal of Clinical Pediatric Dentistry. 2017; 41(4): 284-288. doi: 10.17796/1053-4628-41.4.284

References

1. Siqueira, J.F., I.N. Rôças, A. Favieri, and K.C. Lima. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. Journal of Endodontics 26:331-334, 2000

2. Rodd, H.D., et al. Pulp therapy for primary molars. Int J Paediatr Dent 16 Suppl 1:15-23, 2006

3. Sato, T., E. Hoshino, H. Uematsu, and T. Noda. Predominant obligate anaerobes in necrotic pulps of human deciduous teeth. Microbial ecology in health and disease 6:269-275, 1993

4. Topcuoglu, N., E. Bozdogan, and G. Kulekci. Presence of oral bacterial species in primary endodontic infections of primary teeth. J Clin Pediatr Dent 38:155-160, 2013

5. Sundqvist, G. Taxonomy, ecology, and pathogenicity of the root canal flora. Oral Surg Oral Med Oral Pathol 78:522-530, 1994

6. Bhangdia, M.B., B. Nandlal, G.S. Vijaykumar, P.K. Kulkarni, and R. Shan-bhog. Clinical evaluation of sustained-release metronidazole gel versus metronidazole solution as an intracanal medicament in abscessed primary molars. Eur Arch Paediatr Dent 15:19-26, 2014

7. Önçag̈ , Ö., D. Gogulu, and A. Uzel. Efficacy of various intracanal medica-ments against Enterococcus faecalis in primary teeth: an in vivo study. Journal of Clinical Pediatric Dentistry 30:233-237, 2006

8. Gondim, J.O., et al. Effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against Porphyromonas gingivalis and Enterococcus faecalis. Int J Paediatr Dent 22:116-124, 2012

9. Zehnder, M. Root canal irrigants. J Endod 32:389-398, 2006

10. Becking, A.G. Complications in the use of sodium hypochlorite during endodontic treatment. Report of three cases. Oral Surg Oral Med Oral Pathol 71:346-348, 1991

11. Nogales, C.G., P.H. Ferrari, E.O. Kantorovich, and J.L. Lage-Marques. Ozone therapy in medicine and dentistry. J Contemp Dent Pract 9:75-84, 2008

12. de Gregorio, C., R. Estevez, R. Cisneros, A. Paranjpe, and N. Cohenca. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. Journal of endodontics 36:1216-1221, 2010

13. Bocci, V.A. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 37:425-435, 2006

14. Nagayoshi, M., C. Kitamura, T. Fukuizumi, T. Nishihara, and M. Terashita. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 30:778-781, 2004

15. Desai, P. and V. Himel. Comparative safety of various intracanal irrigation systems. J Endod 35:545-549, 2009

16. Boutsioukis, C., et al. Evaluation of irrigant flow in the root canal using different needle types by an unsteady computational fluid dynamics model. J Endod 36:875-879, 2010

17. Schoeffel, G.J. The EndoVac method of endodontic irrigation: safety first. Dent Today 26:92, 94, 96 passim, 2007

18. Miller, T.A. and J.C. Baumgartner. Comparison of the antimicrobial efficacy of irrigation using the EndoVac to endodontic needle delivery. J Endod 36:509-511, 2010

19. Iriboz, E., K. Bayraktar, D. Turkaydin, and B. Tarcin. Comparison of apical extrusion of sodium hypochlorite using 4 different root canal irrigation techniques. J Endod 41:380-384, 2015

20. Yucel, A.C., M. Gurel, E. Guler, and B. Karabucak. Comparison of final irrigation techniques in removal of calcium hydroxide. Aust Endod J 39:116-121, 2013

21. Ahmed, H.M. Anatomical challenges, electronic working length determi-nation and current developments in root canal preparation of primary molar teeth. Int Endod J 46:1011-1022, 2013

22. Liu, H., X. Wei, J. Ling, W. Wang, and X. Huang. Biofilm formation capa-bility of Enterococcus faecalis cells in starvation phase and its suscepti-bility to sodium hypochlorite. J Endod 36:630-635, 2010

23. Lin, J., Y. Shen, and M. Haapasalo. A comparative study of biofilm removal with hand, rotary nickel-titanium, and self-adjusting file instrumentation using a novel in vitro biofilm model. J Endod 39:658-663, 2013

24. Ricucci, D. and J.F. Siqueira, Jr. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod 36:1277-1288, 2010

25. Fanning, E.A. Most cited: number 1. A longitudinal study of tooth forma-tion and root resorption. N Z Dent J 104:60-61, 2008

26. Parente, J.M., et al. Root canal debridement using manual dynamic agita-tion or the EndoVac for final irrigation in a closed system and an open system. Int Endod J 43:1001-1012, 2010

27. Kustarci, A., Z. Sumer, D. Altunbas, and S. Kosum. Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107:e73-79, 2009

28. Siqueira, J.F., Jr., I.N. Rocas, A. Favieri, and K.C. Lima. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod 26:331-334, 2000

29. Tay, F.R., et al. Effect of vapor lock on root canal debridement by using a side-vented needle for positive-pressure irrigant delivery. J Endod 36:745-750, 2010

30. Canoglu, H., M.U. Tekcicek, and Z.C. Cehreli. Comparison of conven-tional, rotary, and ultrasonic preparation, different final irrigation regimens, and 2 sealers in primary molar root canal therapy. Pediatr Dent 28:518-523, 2006

31. Estrela, C., C.R. Estrela, D.A. Decurcio, A.C. Hollanda, and J.A. Silva. Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypo-chlorite and chlorhexidine in infected human root canals. Int Endod J 40:85-93, 2007

32. Goztas, Z., H. Onat, G. Tosun, Y. Sener, and H.H. Hadimli. Antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals. Eur J Dent 8:469-474, 2014

33. Brito, P.R., et al. Comparison of the effectiveness of three irrigation tech-niques in reducing intracanal Enterococcus faecalis populations: an in vitro study. J Endod 35:1422-1427, 2009

34. Nakornchai, S., P. Banditsing, and N. Visetratana. Clinical evaluation of 3Mix and Vitapex as treatment options for pulpally involved primary molars. Int J Paediatr Dent 20:214-221, 2010

35. Barcelos, R., P.N. Tannure, R. Gleiser, R.R. Luiz, and L.G. Primo. The influence of smear layer removal on primary tooth pulpectomy outcome: a 24-month, double-blind, randomized, and controlled clinical trial evalua-tion. Int J Paediatr Dent 22:369-381, 2012

36. Miranda, R.G., E.B. Santos, R.M. Souto, H. Gusman, and A.P. Colombo. Ex vivo antimicrobial efficacy of the EndoVac system plus photodynamic therapy associated with calcium hydroxide against intracanal Enterococcus faecalis. Int Endod J 46:499-505, 2013

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