Article Data

  • Views 670
  • Dowloads 162

Original Research

Open Access

Clinical Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation

  • Osama Safwat1
  • Mona Elkateb2
  • Karin Dowidar3
  • Omar El Meligy3,*,

1Ministry of Health, Alexandria, Egypt

2College of Dentistry, Princess Nora bint Abdulrahman University, Saudi Arabia

3Faculty of Dentistry, Alexandria University, Egypt.

DOI: 10.17796/1053-4628-41.6.3 Vol.41,Issue 6,November 2017 pp.429-441

Published: 01 November 2017

*Corresponding Author(s): Omar El Meligy E-mail: omeligy@kau.edu.sa

Abstract

To evaluate the clinical changes in dentin of deep carious lesions in young permanent molars, following ozone application with and without the use of a remineralizing solution, using the stepwise excavation. Study design: The sample included 162 first permanent immature molars, showing deep occlusal carious cavities that were indicated for indirect pulp capping. Teeth were divided into 2 main groups according to the method of ozone treatment. Each group was further subdivided equally into test and control subgroups. Following caries excavation, color, consistency and DIAGNOdent assessments of dentin were evaluated after 6 and 12 months. Results: Regarding dentin color and consistency, no significant differences were observed following ozone application, with and without a remineralizing solution. There were no significant differences between ozone treatment, and calcium hydroxide during the different evaluation periods, except in group I cases after 6 months, concerning the dentin color. The DIAGNOdent values were significantly reduced following ozone application, with or without a remineralizing solution, as well as between test and control cases in group I after 6 months. Conclusions: Ozone application through the stepwise excavation had no significant effect on dentin color and consistency in young permanent molars. DIAGNOdent was unreliable in monitoring caries activity.

Keywords

Ozone, Stepwise excavation, Young permanent molars.

Cite and Share

Osama Safwat,Mona Elkateb,Karin Dowidar,Omar El Meligy. Clinical Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation. Journal of Clinical Pediatric Dentistry. 2017. 41(6);429-441.

References

1-American Academy of Pediatric Dentistry. Guideline on pulp therapy for primary and immature permanent teeth. Ped Dent, 37: 244-52, 2015.

2- Gomez J. Detection and diagnosis of the early caries lesion. BMC Oral Health, 15 Suppl 1: S3. doi: 10.1186/1472-6831-15-S1-S3, 2015.

3- Souza JF, Boldieri T, Diniz MB, Rodrigues JA, Lussi A, Cordeiro RC. Traditional and novel methods for occlusal caries detection: performance on primary teeth. Lasers Med Sci, 28: 287-95, 2013.

4- Alwas-Danowska HM, Plasschaert AJ, Suliborski S, Verdonschot EH. Reliability and validity issues of laser fluorescence measurements in occlusal caries diagnosis. J Dent, 30: 129-34, 2002.

5- Thompson V, Craig RG, Curro FA, Green WS, Ship JA. Treatment of deep carious lesions by complete excavation or partial removal: A critical review. J Am Dent Assoc, 139: 705-12, 2008.

6- Bjorndal L, Larsen T, Thylstrup A. A clinical and microbiological study of deep carious lesions during stepwise excavation using long treatment intervals. Caries Res, 31: 411-17, 1997.

7- Grootveld M, Baysan A, Sidiiqui N, Sim J, Silwood C, Lynch E. History of the clinical applications of Ozone. In: Lynch E. Editor. Ozone: The revolution in dentistry. London: Quintessence Publishing Co; 24, 2004.

8- Bocci V1, Zanardi I, Travagli V. Oxygen/ozone as a medical gas mixture. A critical evaluation of the various methods clarifies positive and negative aspects. Med Gas Res, 1:6. doi: 10.1186/2045-9912-1-6, 2011.

9- Lynch E, Silwood CJ, Abu-Naba’a L, Al Shorman H, Baysan A, Holmes J, Grootveld M. Oxidative consumption of root caries biomolecules using Ozone. Caries Res, 38: 364, 2004.

10- Almaz ME, Sönmez IŞ. Ozone therapy in the management and prevention of caries. J Formos Med Assoc, 114: 3-11, 2015.

11- Abu-Naba’a L, Al Shorman H, Holmes J, Petersson LG, Tagami J, Lynch E. Evidence-based research into ozone treatment in dentistry: An overview. In: Lynch E. Editor. Ozone: The revolution in dentistry. London: Quintessence Publishing Co; 96-102, 2004.

12- Zhong B, Peng C, Wang G, Tian L, Cai Q, Cui F. Contemporary research findings on dentine remineralization. J Tissue Eng Regen Med, 9: 1004-16, 2015.

13- Kawasaki K, Ruben J, Tsuda H. Relationship between mineral distributions in dentin lesions and subsequent remineralization in vitro. Caries Res, 34: 395-403, 2000.

14- Mukai Y, Ten Cate J M. Remineralization of advanced root dentin lesions in vitro. Caries Res, 36: 275-80, 2002.

15- Kevin J. Douglas J: Identify, protect, restore: Emerging issues in approaching children’s oral health. General Dent, 35: 106-10, 2005.

16- KaVo DIAGNOdent 2095. Clinical guidelines and operating instructions device. 2001.

17- Ekstrand KR, Ricketts DN, Kidd EA, Qvist V, Schou S. Detection, diagnosing, monitoring and logical treatment of occlusal caries in relation to lesion activity and severity. An in vivo examination with histological validation. Caries Res, 32: 247-54, 1998.

18- Lussi A, Imwinkelried S, Pitts NB, Longbottom C, Reich E. Performance and reproducibility of a laser fluorescence system for detection of occlusal caries in vitro. Caries Res, 33: 261-66, 1999.

19- Holmes J, Lynch E. Equipment available to deliver ozone in dentistry. In: Lynch E. editor. Ozone: The revolution in dentistry. London: Quintessence Publishing Co; 57-65, 2004.

20- Stockleben C. HealOzone a revolution in dentistry. In: Lynch E. Editor. Ozone: The revolution in dentistry. London: Quintessence Publishing Co; 238, 2004.

21- Moore DS, McCabe GP, Craig BA. Introduction to the practice of Statistics. 8th ed. New York: WH Freeman Company; 2014.

22- Lager A, Thornqvist E, Ericson T. Cultivatable bacteria in dentin after caries excavation using rose-bur or Carisolv. Caries Res, 37: 206-11, 2003.

23- Hilton T, Summitt JB. Pulpal Considerations. In: Summitt JB, Robbins JW, Schwartz RS. Editors. Fundamentals of Operative Dentistry. 3rd ed. London: Quintessence publishing Co; 103, 2000.

24- Dodds MW. Dental caries diagnosis toward the 21st century. Nat Med, 2 :283, 1996.

25- Côrtes DF, Ekstrand KR, Elias-Boneta AR, Ellwood RP. An in vitro comparison of the ability of fiber-optic transillumination, visual inspection and radiograph to detect occlusal caries and evaluate lesion depth. Caries Res, 34: 443-7, 2000.

26- Lussi A, Megert B, Longbottom C, Reich E, Francescut P. Clinical performance of a laser fluorescence device for detection of occlusal caries lesions. Eur J Oral Sci, 109: 14-9, 2001.

27- Bjørndal L. Indirect pulp therapy and stepwise excavation. Pediatr Dent, 30: 225-9, 2008.

28- Fuks AB, Heling I, Nuni E. Pulp therapy for the young permanent dentition. In: Casamassimo PS, Fields HW, McTigue DJ, Nowak AJ. Editors. Pediatric Dentistry: Infancy through adolescence. 5th ed. Missouri: Elsevier Inc; 490, 2013.

29- Hasselgren G: Treatment of the exposed dentin-pulp complex. In Orstavik D, Pitt Ford TR. Editors. Essential Endodontology: Prevention and Treatment of Apical Periodontitis. Oxford: Blackwell Science Inc; 192-210, 1998.

30- Smith C, Lynch F, Baysan A, Silwood CJ, Mills B, Grootveld M. Oxidative consumption of root caries biomolecules by a novel antibacterial ozone delivery system. J Dent Res, 80: 1178 (abstract), 2001.

31- Lynch E, Silwood CJ, Grootveld M. Oxidizing actions of an antibacterial ozone-generating device towards root caries biomolecules. J Dent Res, 81: 138 (abstract), 2002.

32- Baysan A, Lynch E. Management of root caries using ozone in-vivo. J Dent Res, 80: 37-9, 2001.

33- Dominguez MS, Witherspoon DE, Gutmann JL, Opperman LA. Histological and scanning electron microscopy assessment of various vital pulptherapy materials. J Endod, 29: 324-33, 2003.

34- Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci, 114: 435-40, 2006.

35- Maltz M, Oliveira EF, Fontanella V, Bianchi R. A clinical, microbiologic, and radiographic study of deep caries lesions after incomplete caries removal. Quintessence Int, 33: 151-9, 2002.

36- Tziafas D, Kodonas K: Differentiation potential of dental papilla, dental pulp, and apical papilla progenitor cells, J Endod, 36: 781-9, 2010.

37- Wambier DS, Dos Santos FA, Guedes-Pinto AC, Jaeger RG, Simionato MR. Ultrastructural and microbiological analysis of the dentin layers affected by caries lesions in primary molars treated by minimal intervention. Pediatr Dent, 29: 228-34, 2007.

38- Andrian S, Iovan G, Georgescu A. Remineralisation of affected dentin by different bioactive materials in the stepwise excavation technique. Stoma Edu J, 1: 12-17, 2014.

39- Stock C. Calcium hydroxide: root resorption and perio-endo lesions. Br Dent J, 158: 325–34, 1985.

40- Farhad A, Mohammadi Z. Calcium hydroxide: a review. Int Dent J, 55: 293-301, 2005.

41- Graham L, Cooper PR, Cassidy N, Nor JE, Sloan AJ, Smith AJ. The effect of calcium hydroxide on solubilisation of bio-active dentin matrix components. Biomaterials, 27: 2865-73, 2006.

42- De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, Van Meerbeek B. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res, 84: 118-32, 2005.

43- Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci, 114: 435-40, 2006.

44- Dahnhardt JE, Jaeggi T, Lussi A. Treating open carious lesions in anxious children with ozone: a prospective controlled clinical study. Am J Dent, 19: 267-70, 2006.

45- Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci, 114: 349-53, 2006.

46- Iwami Y, Shimizu A, Yamamoyo H, Hayashi M, Takeshinge F, Ebisu S. In vitro study of caries detection through sound dentin using a laser fluorescence device, DIAGNOdent. Eur J Oral Sci, 111: 7-11, 2003.

47- Iwami Y, Shimizu A, Narimatsu M. Relationship between bacterial infection and evaluation using a laser fluorescence device, DIAGNOdent. Eur J Oral Sci, 112: 419-23, 2004.

48- Krause F, Braun A, Eberhard J, Jepsen S. Laser fluorescence measurements compared to electrical resistance of residual dentin in excavated cavities in vivo. Caries Res, 41: 135-40, 2007.

49- Suzaki A, Takahashi K, Sakuma K, Arimoto N, Senda A, Hayakava T. A fundamental study on clinical application of DIAGNOdent -Effects of demineralization and collagenase treatment of bovine dentin on the defective response of DIAGNOdent. Jpn J Conser Dent, 43: 200-7, 2000.

50- Tziafas D, Smith AJ, Lesot H. Designing new treatment strategies in vital pulp therapy. J Dent, 28: 77-92, 2000.

51- Unlu N, Ermis RB, Sener S, Kucukyilmaz E, Cetin AR. An in-vitro comparison of different diagnostic methods in detection of residual dentinal caries. Int J Dent, 2010:864935. doi: 10.1155/2010/864935, 2010.

52- Bamzahim M, Shi XQ, Angmar-Mansson B. Secondary caries detection by DIAGNOdent and radiography. A comparative in vitro-study. Acta Odontol Scand, 62: 61-4, 2004.

53- Souza- Zaroni WC, Ciccone JC, Souza-Gabriel AE, Ramos RP, Corona SA, Palma-Dibb RG. Validity and reproducibility of different combinations of methods for occlusal caries detection: an in vitro comparison. Caries Res, 40: 194-201, 2006.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

PubMed (MEDLINE) PubMed comprises more than 35 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full text content from PubMed Central and publisher web sites.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Scopus: CiteScore 2.0 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Submission Turnaround Time

Conferences

Top