Periodontal Pathogen Colonization in Young Children by PCR Quantification – A Longitudinal Survey

Periodontal diseases are among the leading causes of premature tooth loss in adults, but the microbiota associated with this problem is established over time in childhood. Aim: This longitudinal study aimed to verify the occurrence of periodontal pathogens in the oral cavity of children aged six, twelve, eighteen and twenty-four months through PCR quantification, correlating them with the oral microbiota of their mothers. Study design: Saliva and oral biofilm samples were collected from mothers and children by using sterilized paper points. Furthermore, a questionnaire was applied in all periods to evaluate hygiene and dietary habits. Results: A positive correlation was found between mother–child pairs in all periods. No correlation was observed between hygiene and dietary habits and occurrence of periodontal pathogens. Conclusion: Early inclusion of children in preventive and biofilm control programs could contribute to preventing acquisition of aggressive pathogens.

Saliva; Anaerobic Bacteria; Periodontal Diseases.

1. Cunha RF; Delbem ACB; Percinoto C. Dentistry for babies: a preventive protocol. ASDC J Dent Child; 67:89- 92. 2000.

2. Albandar JM; Rams TE. Global epidemiology of periodontal diseases: an overview. Periodontol; 29:7- 10. 2002.

3. Sakai VT; Campos MR; Machado MAAM et al. Prevalence of four putative periodontopathic bacteria in saliva of a group of Brazilian children with mixed dentition: 1-year longitudinal study. Int J Paediatr; 17:192-199. Dent 2007.

4. Haraszthy VI; Jordan SF; Zambon JJ. Molecular cloning of the fur gene from Actinobacillus Actinomycetemcomitans. Infect Immun; 70:3170-3179. 2002.

5. Miura M; Hamachi T; Fujise O et al. The prevalence and pathogenic differences of Porphyromonas gingivalis fimA genotypes in patients with aggressive periodontitis. J Periodontal Res; 40:147-152. 2005.

6. Yang EY; Tanner ACR; Milgrom P et al. Periodontal pathogen detection in gingival/ tooth and tongue flora samples from 18- to 48- month- old children and periodontal status of their mothers. Oral Microbiol Immunol; 17:55- 59. 2002.

7. Yildirin S; Yapar M; Kubar A. Detection and quantification of herpesviruses in Kostmann syndrome periodontitis using real-time polymerase chain reaction: a case report. Oral Microbiol Immunol; 21:73- 78. 2006.

8. Dogan B; Kipalev AS; Okte E et al. Consistent intrafamilial transmission of Actinobacillus actinomycetemcomitans despite clonal diversity. J Periodontol; 79:307-315. 2008.

9. Könönen E; Wolf J; Matto J et al. The Prevotella intermedia group organisms in young children and their mothers as related to maternal periodontal status. J Periodontal Res; 35:329-334. 2000.

10. Watson MR; Bretz WA; Loesche WJ. Presence of Treponema denticola and Porphyromonas gingivalis in children correlated with periodontal disease of their parents. J Dent Res; 73:1636- 1640. 1994.

11. Watson MR; Bretz WA; Loesche WJ. Detection of two anaerobic periodontopathogens in children by means of the BANA and ELISA assays. J Dent Res;70:1052-1056. 1991.

12. Oh TJ; Eber R; Wang HL. Periodontal diseases in the child and adolescent. J Clin Periodontol; 29:400-410. 2002.

13. Jardim Junior EG; Bosco JMD; Lopes AM; Landucci LF; Jardim ECG; Carneiro SRS. Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil. J Appl Oral Sci; 14:153-6. 2006.

13. Caulfield P W; Cutter G R; Dasanayake A P. Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J Dent Res; 72: 37- 45. 1993.

14. Papaioannou W; Gizani S; Hafajee AD. The microbiota on different oral surfaces in healthy children. Oral Microbiol Immunol; 24: 183-189. 2009.

15. Busscher HJ; Evans L V. Oral biofilm and plaque control. Amsterdam: Harwood Academic, 1998. 348 p.

16. Sarkonen N. Oral Actinomyces species in health and disease: identification, occurrence and importance of early colonization. 2007. 57 f. Dissertation– Faculty of Medicine, University of Helsinki, Helsinki, 2007.

17. Tanner ACR; Pasteur BJ; Lu SC et al. Similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. Oral Microbiol Immunol; 17:379-87. 2002.

18. Cole M F ; Bryan S ; Evans MK et al. Humoral immunity to commensal oral bacteria in human infants: salivary antibodies reactive with Actinomyces naeslundii genospecies 1 and 2 during colonization. Infect Immun; 66:4283-4289. 1998.

19. Umeda M; Miwa Z; Takeuchi Y. The distribution of periodontopathic bacteria among Japanese children and their parents. J Periodontal Res; 39:398-404. 2004.

20. Cortelli JR; Aquino DR; Cortelli SC et al. Etiological analysis of initial colonization of periodontal pathogens in oral cavity. J Clin Microbiol; 46:1322–1329. 2008.

21. Okada M; Hayashi F; Nagasaka N. Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age. J. Clin Periodontol;27:763-768. 2002.

22. Ramseier CA; Kinney JS; Herr AE et al. Identification of pathogen and host-response markers correlated with periodontal disease. J. Periodontol; 80:436-446. 2009.

23. Lafaurie GI ; Contreras A ; Baron A et al. Demographic, clinical and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J Periodontol 2007; 78:629-639.

24. Colombo APV; Haffajee AD; Smith CM et al. Subgingival microbiota of Brazilian subjects with untreated chronic periodontitis. J Periodontol; 73:360-369. 2002.

25. Tamura K; Nakano K; Hayashibara T et al. Distribution of 10 periodontal bacteria in saliva samples from Japanese children and their mothers. Arch Oral Biol; 51:371-377. 2006.

26. Haffajee AD; Bogren A; Hasturk H et al. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol;31:996-1002. 2004.

27. Baumgartner S; Imfeld T; Schicht O et al. The impact of the stone age diet on gingival conditions in the absence of oral hygiene. J Periodontol;80:759-768. 2009.

28. Frisken KW; Higgins T; Palmer JM. The incidence of periodontopathic microorganisms in young children. Oral Microbiol. Immunol;5:.43-45. 1990.

29. Haraldsson G; Holbrook WP; Könönen E. Clonal persistence of oral Fusobacterium nucleatum in infancy. J Dent Res; 83:500- 504. 2004.

30. Kolenbrander PE; London J. Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol;175:3247- 3252. 1993.

31. Socransky SS ; Haffajee AD; Smith C et al. Microbial complexes in subgingival plaque. J Clin Periodontol; 25:134-144. 1998.

32. Feng Z; Weinberg A. Role of bacteria in health and disease of periodontal tissues. Periodontol 2000 2006; 40:50- 76.

33. Matto J; Saarela M; Alaluusua S et al. Detection of Porphyromonas gingivalis from saliva by PCR by using a simple-sample processing method. J Clin Microbiol; 36:157- 160. 1998.

34. Kulekci G; Leblebibioglu B; Keskin F et al. Salivary detection of periodontopathic bacteria in periodontally healthy children. Anaerobe;14: 49- 54. 2008.

35. Gafan GP; Lucas VS; Robert GJ et al. Prevalence of periodontal pathogens in dental plaque of children. J Clin Microbiol; 42:4141-4146. 2004.

36. Carvalho RPM; Mesquita JS; Bonomo A et al. Relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis. Oral Microbiol Immunol; 24:124-132. 2009.

37. Nakano K; Miyamoto E; Tamura K et al. Distribution of 10 periodontal bacterial species in children and adolescents over a 7-year period. Oral Dis;14: 658-664. 2008.

38. Grindefjord M; Dahlof G; Wikner S et al. Prevalence of mutans streptococci in one-year-old children. Oral Microbiol Immunol;6:280-283. 1991.