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Original Research

Open Access

Microbiological Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation

  • Osama Safwat1
  • Mona Elkateb2
  • Karin Dowidar3
  • Hala Abdel Salam3
  • Omar El Meligy4,*,

1Consultant of Pediatric Dentistry, Ministry of Health, Alexandria, Egypt

2Faculty of Dentistry, Alexandria University, Egypt and Professor of Pediatric Dentistry, College of Dentistry, Princess Nora bint Abdulrahman University, Saudi Arabia

3Faculty of Dentistry, Alexandria University, Egypt

4Faculty of Dentistry, King Abdulaziz University, Saudi Arabia and Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Egypt

DOI: 10.17796/1053-4628-42.1.3 Vol.42,Issue 1,January 2018 pp.11-20

Published: 01 January 2018

*Corresponding Author(s): Omar El Meligy E-mail:


Aim: To assess the microbial effect of ozone gas on dentinal lesions in young permanent molars using the stepwise excavation. Study design: An experimental, controlled clinical trial was performed. The sample included 80 immature first permanent molars, showing deep occlusal carious cavities that were indicated for stepwise excavation. Following first step of dentin excavation, the sample was divided into test (ozone gas) and control (calcium hydroxide (Ca(OH)2) base material) groups. One half of the cases in each group were evaluated for microbiological changes after 6 months, and the other half after 12 months. Results: Mutans streptococci (MS), Lactobacilli, and Candida counts were significantly reduced immediately after ozone application in the test group (P ≤0.05). At the final assessment period, MS and Lactobacilli were significantly reduced in the test group (P ≤0.05). Meanwhile, the Candida counts were significantly reduced only in the test group of the 6 and 12 month-cases (P ≤0.05). Regarding the control group, the significant reduction in microbial count was observed with MS after 6 and 12 months (P ≤0.05). No significant differences were observed between test and control groups at different evaluation periods (P >0.05). Conclusions: Ozone gas had a significant antimicrobial effect in deep class I carious lesions.


Ozone, Stepwise excavation, Young permanent molars

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Osama Safwat,Mona Elkateb,Karin Dowidar,Hala Abdel Salam,Omar El Meligy. Microbiological Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation. Journal of Clinical Pediatric Dentistry. 2018. 42(1);11-20.


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