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Original Research

Open Access

Periodontal Pathogen Colonization in Young Children by PCR Quantification – A Longitudinal Survey

  • Karine Takahashi1,*,
  • Robson Frederico Cunha2
  • Elerson Gaetti Jardim Junior2

1Pediatric Dentistry at Presidente Prudente Dental School, West São Paulo University

2,Pediatric Dentistry at São Paulo State University Araçatuba Dental School

DOI: 10.17796/1053-4628-42.2.4 Vol.42,Issue 2,March 2018 pp.103-108

Published: 01 March 2018

*Corresponding Author(s): Karine Takahashi E-mail:


Introduction: Periodontal diseases are among the leading causes of premature tooth loss in adults, but the microbiota associated with this problem is established over time in childhood. Aim: This longitudinal study aimed to verify the occurrence of periodontal pathogens in the oral cavity of children aged six, twelve, eighteen and twenty-four months through PCR quantification, correlating them with the oral microbiota of their mothers. Study design: Saliva and oral biofilm samples were collected from mothers and children by using sterilized paper points. Furthermore, a questionnaire was applied in all periods to evaluate hygiene and dietary habits. Results: A positive correlation was found between mother–child pairs in all periods. No correlation was observed between hygiene and dietary habits and occurrence of periodontal pathogens. Conclusion: Early inclusion of children in preventive and biofilm control programs could contribute to preventing acquisition of aggressive pathogens


Saliva; Anaerobic Bacteria; Periodontal Diseases

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Karine Takahashi,Robson Frederico Cunha,Elerson Gaetti Jardim Junior. Periodontal Pathogen Colonization in Young Children by PCR Quantification – A Longitudinal Survey. Journal of Clinical Pediatric Dentistry. 2018. 42(2);103-108.


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