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Original Research

Open Access

Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham’s F-10 Solution

  • Maryam Talebi1
  • Iman Parisay1,*
  • Jalil Tavakol afshari2
  • Arezoo Shajiei3
  • Mostafa Sofiani Ghadim4

1Dental Material Research Center, School of Dentistry, Mashhad University of Medical Sciences, Iran

2Department of Immunogenetic and Tissue Culture, Mashhad University of Medical Sciences

3Cancer Molecular Pathology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Iran

4Pediatric Dentist, Mashhad University of Medical Sciences, Iran

DOI: 10.17796/1053-4628-42.3.6 Vol.42,Issue 3,May 2018 pp.203-207

Published: 01 May 2018

*Corresponding Author(s): Iman Parisay E-mail: parisayi@mums.ac.irparisay iman@gmail.com

Abstract

Objectives: The purpose of the present study was to evaluate the efficacy of Ham's F-10 in maintaining the viability and reproducibility of PDL cells on avulsed teeth. Study design: Sixty mature, healthy extracted premolars were used. The experimental media used were Ham's F-10, Hank's balanced salt solution (HBSS), skim milk, and tap water (n = 15 specimens each). Cell viability was tested after 1, 3, 6, and 24 h storage in medium. Cell reproducibility was assessed by methyl-thiazol-tetrazolium (MTT) assay after1, 3, and 6 h storage in Ham's F-10, HBSS, and tap water. Results: The viability of PDL cells stored in Ham's F-10 and HBSS was significantly greater than that of samples stored in milk and tap water at all-time points (P<0.001). A significant difference in cell viability between samples stored in Ham's F-10 and HBSS (favoring the former) was observed only at 6h (P=0.04). MTT assay results were significantly better for samples stored in Ham's F-10 and HBSS than for those stored in tap water (P<0.001), with a significant difference between Ham's F-10 and HBSS observed only at 3h (P<0.001).

Conclusions: Ham's F-10 is capable of preserving PDL cells viable and reproducible better than milk and tap water and similar to HBSS.

Keywords

avulsed tooth, Ham’s F-10 cell culture medium, Hank’s balanced salt solution, periodontal ligament cell, reproducibility, storage media, and viability

Cite and Share

Maryam Talebi,Iman Parisay,Jalil Tavakol afshari,Arezoo Shajiei,Mostafa Sofiani Ghadim. Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham’s F-10 Solution. Journal of Clinical Pediatric Dentistry. 2018. 42(3);203-207.

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