Dental Diseases and Intestinal Dysbiosis Among Children

Objectives: The goal of the present study correlates dental hard tissue mineralization, mucosal pathologies in the oral cavity and different degrees of intestinal dysbiosis. Study Design: the study examined two groups: the study group (Group I) included 229 children and adolescents aged 1–17 (mean age 5±1years) with oral pathologies (caries, acute or chronic candidiasis) and confirmed dysbiosis of varying severity and stages as well. Group II (the Control Group) was composed of 50 patients aged 1 – 16 (mean age 5±1years) with oral pathologies but with no detected changes in gastrointestinal (GI) flora. Dental caries were examined by DMFT-index; the extent of dental hard tissue mineralization by vital staining (2% methylene blue) and cases of oral candidiasis was investigated by taking cultures from mucosal plaques. Results: on the basis of the research outcomes the correlation between the different degrees of GI dysbiosis and dental hard tissue mineralization with pathologic expressions in the oral cavity was found. Group I was divided into two subgroups: in the first subgroup that suffered from mild dysbiosis (I and II degree) moderate dental caries was revealed, whereas in the second subgroup with III and IV degree of dysbiosis–high levels of dental caries was detected. In Group II (no GI flora disturbances), the dental hard tissue demineralization indicator was minimal; in children aged 1–3 years the incidence and prevalence of caries were low and increased with age, reaching higher values during puberty (11–16 years). Conclusion: It may be concluded that dysbiosis of GI microflora influences on a degree of dental hard tissue demineralization, which in turn may predispose to the formation of dental caries.

intestinal dysbiosis in children, dental hard tissues, dental caries

1. Shishniashvili T. Dental Disease Prophylaxis. Ed. Tbilisi, Tbilisi; 3 – 4. 2012

2. Makhviladze M, Zubadalashvili M. Comparative Effectiveness of Linex and Lacto-G during Dysbiosis in Adults. Georgian Medical News; 35:45-

49. 2009

3. Tamboli G, Neut C, Desreumaux P, Colombel J.F. Dysbiosis in Inflamma-tory Bowel Disease. Gut; 53:1-4. 2004

4. Hawrelak J.A, Myers S.P. The causes of intestinal dysbiosis. Altern. Med. Rev; 9(2): 180-197. 2004

5. Torrazza R.M, Ukhanova M, Wang X, Sharma R, Hudak M.L, Neu J, Mai V. Intestinal microbial ecology and environmental factors affecting necrotizing enterocolitis. PLoS ONE; 8(12):e83304. 2013

6. De Vrese M, Marteau PH.K. Probiotics and Prebiotics Effects on Diar-rhea. J. Nutr; 137:803-811. 2007

7. Koning C.J, Jonkers D.M, Stobberingh E.E, Mukder L, et al. The Effect of a Multispecies Probiotic on the Intestinal Microbiota and Bowel Movements in Healthy Volunteers Taking the Antibiotic Amoxycillin. American Journal of Gastroenterology; 103(1):178-189. 2008

8. Milward M.R. Important Oral Bacteria: The Oral Microflora; School of Dentistry: 19-21. 2012

9. Gill H, Prasad J. Probiotics, Immunomodulation and Health Benefits. Advances in Experimental Medicine and Biology; 606:423-54. 2008

10. Vangay P, Ward T, Gerber J.S., Knights D. Antibiotics, pediatric dysbi-osis, and disease. Cell Host & Microbe; 17(5):553-564. 2015

11. SC Kim, GD Ferry. Inflammatory Bowel Diseases In Pediatric and Adolescent Patients: Clinical, Therapeutic, and Psychosocial Consider-ations. Gastroenterology, Elsevier; 2004

12. Bruttin A. & Brüssow H. Human Volunteers Receiving Escherichia coli Phage T4 Orally: A Safety Test of Phage Therapy. Antimicrobial Agents Chemotherapy; 49(7):2874-2878. 2005

13. Chanishvili N. et al. Phages and their application against drug resistant bacteria. J Chem Technol Biotechnol; 76:1

14. Malmö University. Methods and Indices for Caries prevalence [https://www.mah.se/CAPP/Methods-and-Indices/for-Caries-prevalence/], accessed 12 May 2014.

15. Malmö University. Simplified Oral Hygiene Index (OHI-S) [https://www. mah.se/CAPP/Methods-and-Indices/Oral-Hygiene-Indices/Simplified-Oral-Hygiene-Index—OHI-S/], accessed 5 February 2016.

16. Statement of Ministry of Health of Russian Federation no. 231. Moscow; 112, 2003

17. Jason A. Hawrelak and Stephen P. Myers. The Causes of Intestinal Dysbi-osis: A Review. Alternative Medicine Review; 9 (2):180-197. 2004

18. Fischett V.A., Domiel N. & Raymond S. Reinventing phage therapy: Are the parts greater than the sum?. Nature Biotechnology; 24:1508-1511. 2006

19. Skurnik M. & Staunch E. Phage therapy: Facts and fiction. International Journal of Microbiology; 296(1):5-14. 2006

20. Nancy K, Henry, Jay L, Hoecker K, Hable Rhodes. Antimicrobial Therapy for Infants and Children: Guidelines for the Inpatients and Outpatients Practice of Pediatric Infectious Diseases. Mayo Clinic Proceedings; 75(1):86-97. January 2000

21. Wong, D.M., Blumberg, D.A. & Lowe L.G. Guidelines for the Use of Antibiotics in Acute Upper Respiratory Tract Infections. American Family Physician; 74(6):956-966. 2006

22. Joerner, R.D. Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages. Poultry Science; 82:640-647. 2003