Time of initial acquisition of mutans streptococci by human infants
1Piracicaba School of Dentistry, State University of Campinas, 901, Limeira Avenue, Piracicaba, São Paulo – Brazil, ZIP CODE: 13414-900
DOI: 10.17796/jcpd.28.4.q327704r2425q5h9 Vol.28,Issue 4,October 2004 pp.303-309
Published: 01 October 2004
The aim of this study was to detect and monitor the acquisition of mutans streptococci (MS) in healthy
Brazilian children. Samples of 4 different sites (saliva, tongue dorsum, dental ridges, and dental plaque,
if teeth were present) were collected from 33 edentulous nursery school infants (5.9±1.5 month-old),
using sterilized swabs, bi-monthly for 24 months. Saliva samples from the mothers were collected only
once. After inoculation, and incubation typical morphotype colonies, were isolated and submitted to
amplification by the technique of polymerase chain reaction (PCR) for identification. The PCR
method identified 1667 strains as MS. In 29 of the children’s samples, the first positive culture for MS
occurred at 15.3 ± 4.6 months. At the end of the follow-up period, 77% of the children were classified
as colonized and in 33% MS was found as a transient microorganism. A positive correlation was
found between the time of MS acquisition by the infant and the number of erupted teeth (p<0.0001),
and the time of emergence of the first tooth (p=0.0048). After 24 months, there were no dental caries,
and 77% of children remained caries-free. These results indicate that MS colonization in this sample
of low-income pre-school children may begin earlier than suggested by some investigations.
Flavia Martao Florio,Marlise Inez Klein,Antonio Carlos Pereira,Reginaldo Bruno Goncalves. Time of initial acquisition of mutans streptococci by human infants. Journal of Clinical Pediatric Dentistry. 2004. 28(4);303-309.
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