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Open AccessThe Detection of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay(IBA)
The Detection of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay(IBA)
- Shoji Tanaka1
- Maki Minami2
- Yukio Murakami1
- Takako Ogiwara1
- Kazuhito Seto1
- Masao Shoji1
- Atushi Hirata1
- Sinnosuke Abe2
- Sigeru Watanabe2
- Seiichiro Fujisawa1,*,
1Department of Diagnostic & Therapeutic Sciences, Meikai University School of Dentistry
2The Human Development & Fostering, Meikai University School of Dentistry, Saitama, Japan
*Corresponding Author(s): Seiichiro Fujisawa E-mail: fujisawa @ dent.meikai. ac.jp
Abstract
The present study was to investigate the distribution of typical periodontpathic bacteria (Porphy-romonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans) in tooth, tongue and buccal mucosa plaques in 3 to 17 Year old children. Clinical parameters (Rates of df, d, DMF, and D; plaque and gingival index) for each subject were determined prior to the collection of each site plaque. Three periodontopathic bacteria on each site samples were detected using IBA. The frequency of three bacteria for tooth plaque was higher than that for tongue or buccal mucosa plaque. The frequency of Porphyromonas gingivalis and Prevotella intermedia in supragingival plaques was significantly higher than that of corresponding ones in tongue or buccal mucosa plaques. The three bacteria also occurred more frequently in subjects aged between 10 and 14 years. Periodontopathic bacteria may be enhanced in circumpubertal children.
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Shoji Tanaka,Maki Minami,Yukio Murakami,Takako Ogiwara,Kazuhito Seto,Masao Shoji,Atushi Hirata,Sinnosuke Abe,Sigeru Watanabe,Seiichiro Fujisawa. The Detection of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay(IBA). Journal of Clinical Pediatric Dentistry. 2006. 30(3);251-256.
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