Article Data

  • Views 682
  • Dowloads 147

Original Research

Open Access

Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM

  • Fernanda Nahás Pires Corrêa1,*,
  • Leonardo Eloy Rodrigues Filho2
  • Célia Regina Martins Delgado Rodrigues3

1Pediatric Dentistry - University of São Paulo, Student of the Doctorate in Pediatric Dentistry Course - University of São Paulo

2,Dental Material Department at the University of São Paulo

3,Orthodontics and Pediatric Dentistry Department at the University of São Paulo

DOI: 10.17796/jcpd.32.2.44n2787118133880 Vol.32,Issue 2,March 2008 pp.115-120

Published: 01 March 2008

*Corresponding Author(s): Fernanda Nahás Pires Corrêa E-mail: fenahas@usp.br

Abstract

The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using rotatory instruments and two chemomechanical methods (Papacárie® and Carisolv®), by scanning electron microscopy (SEM). Thirty primary incisors were divided into three groups, according to the caries removal method used, and their residual dentin was examined under SEM (15). After caries removal, 15 of these teeth were restored with Single Bond (3M) adhesive system and Z100 Filtek composite resin (3M). The tags of the replicas were observed under SEM. The chemomechanical caries removal methods (Papacárie®and Carisolv®) formed an amorphous layer, similar to the smear layer and few exposed dentinal tubules;the conventional caries removal method produced a smooth and regular dentinal surface, with typical smear layer and exposed dentinal tubules. All groups showed abundant tag formation. Scanning electron microscopy analysis revealed a difference between dentin treated with rotatory instruments and that treated with chemomechanical methods in spite of the occurrence of a similar tag formation in both groups.

Keywords

chemomechanical removal; dental caries; primary teeth, scanning electron microscopy, carisolv, papacarie

Cite and Share

Fernanda Nahás Pires Corrêa,Leonardo Eloy Rodrigues Filho,Célia Regina Martins Delgado Rodrigues. Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM. Journal of Clinical Pediatric Dentistry. 2008. 32(2);115-120.

References

1. Ericson D, Zimmerman M, Raber H Gotrick B, Bornstein R, Thorell J. Clinical evaluation of efficacy and safety of a new method for chemo-mechanical removal of caries. Caries Res, 33(3): 171–7, 1999.

2. Kuboki Y, Zimmerman M, Raber H, Cotrick B, Bornstein R. Clinical evaluation of efficacy and safety of a new method from chemomechanical removal of carious dentin. J Dent Res, 56: 1233–7, 1977.

3. Munshi AK, Hedge AM, Shetty PK. Clinical evaluation of Carisolv in the chemico-mechanical removal of carious dentin. J Clin Pediatr Dent, 26(1): 49–54, 2001.

4. Nadanovsky P, Carneiro FC, Mello FS. Removal of carious using only hand instruments: a comparison of mechanical and chemo-mechanical methods. Caries Res, 35(5): 384–9, 2001.

5. Oliveira MDM, Rodrigues CRMD, Wanderley MT, Mathias RS. Uti-lização de método químico- mecânico de remoção do tecido cariado: uma nova proposta para o atendimento de bebês. J Brasileiro de Odon-tologia BP, (13): 209–14, 2003.

6. Kakaboura A, Masouras C, Staikou O, Vougiouklakis G. A comparative clinical study on the Carisolv caries removal method. Quintessence Int, 34(4): 269–71, 2003.

7. 5. Bussadori SK, Castro LC, Galvao AC. Papain gel: a new chemo-mechanical caries removal agent. J Clin Pediatr Dent, 30(2): 115–9, 2005.

8. Tamay TK, Rodrigues CRMD, Mathias RS, Wanderley MT. Avaliação clínico-radiográfica de dentes decíduos tratados com Carisolv [abstract I0 11]. Pesq Odontol Bras, 15: 23, 2001.

9. Rafique S, Fiske J, Banerjee A. Clinical trial of an air-abrasion chemo-mechanical operative procedure for the restorative treatment of dental patients. Caries Res, 37(5): 360–4, 2003.

10. Corrêa FN, Rocha Rde O, Rodrigues Filho LE, Muench A, Rodrigues CR.. Chemical versus conventional caries removal techniques in pri-mary teeth: a microhardness study. J Clin Pediatr Dent, 31(3): 187–92, 2007.

11. Fluckiger L, Waltimo T, Stich H, Lussi A. Comparison of chemome-chanical caries removal using Carisolv or conventional hand excavation in deciduous teeth in vitro. J Dent, 33(2): 87–90, 2005.

12. Fure S, Lingstrom P, Birkhed D. Evaluation of Carisolv for the chemo-mechanical removal of primary root caries in vivo. Caries Res, 34(3): 275–80, 2000.

13. Yazici AR, Atilia P, Ozgunaltay G, Muftuoglu S. In vitro comparison of the efficacy of Carisolv and conventional rotatory instrumental in caries removal. J Oral Rehab, 30(12): 1177–82, 2003.

14. Candido LC. Nova abordagem no tratamento de feridas. São Paulo: SENAC-SP; 2001. http:www.feridologo.com.br/curpapaina.htm [2004 Nov. 23].

15. Giannini M, Reis AF, Arrais CAG. Efeito da profundidade dentinária na resistência de um sistema adesivo autocondicionante. Rev da Posgrad-uacao, 9(1): 43–50, 2002.

16. Pires LAG, Pacheco JFM, Conceição EN. Como obter sucesso clínico com resinas compostas em dentes posteriores. In: Gonçalves EAN, Gentil SN. Atualização clínica em odontologia. São Paulo: Artes Médi-cas 105, 2004.

17. Goldman M, Siu L, White RR, Kronman JA. The dentinal surface of composite restorations after chemo-mechanical caries removal. J Pedod, 12(2): 157–66, 1988.

18. Hanning M. Effect of Carisolv solution on sound, demineralized and denatured dentin- an ultrastructural investigation. Clin Oral Invest, 3(3): 155–9, 1999.

19. Cederlund A, Lindskog S, Blomlof J. Efficacy of Carisolv-assisted caries excavation. Int J Periodontics Restorative Dent, 19(5): 464–9, 1999.

20. Wennerberg A, Sawase T, Kultje C. The influence of Carisolv on enamel and dentin surface topography. Eur J Oral Sci, 107(4): 297–306, 1999.

21. Rodrigues CRMD, Oliveira MDM, Arana-Chavez VE, Mathias RS. S.E.M. study on primary teeth dentin after Carisolv treatment [abstract 196]. J Dent Res, 80(4): 1023, 2001.

22. Splieth C, Rosin M, Gellisen B. Determination of residual dentine caries after conventional mechanical and chemomechanical caries removal with Carisolv. Clin Oral Invest, 5(4): 250–3, 2001.

23. Hosoya Y, Marshall SJ, Watanabe LG, Marshall GW. Microhardness of carious deciduos dentin. Oper Dent, 25(2): 81–9, 2000.

24. Yazici AR, Ozgunaltay G, Dayangaç B. A scanning electron micro-scopic study of different caries removal techniques on human dentin. Oper Dent, 27(4): 360–6, 2002.

25. Sakoolnamarka R, Burrow MF, Tyas MJ. Morphological study of dem-ineralized dentin after caries removal using two different methods. Aust Dent J, 47(2): 116–22, 2002.

26. Dammaschke T, Stratmann U, Morkys K, Kaup M, Ott KHR. Reaction of sound and demineralised dentine to Carisolv in vivo and in vitro. J Dent, 30(1): 50–65, 2002.

27. Tonami K, Araki K, Mataki S, Kurosaki N. Effects of chloramines and sodium hypoclorite on carious dentin. J Med Dent Sci, 50(2): 139–46, 2003.

28. Hossain M, Nakamura Y, Tamaki Y, Yamada Y, Jayawardena JA, Mat-sumoto K. Dentinal composition and knoop hardness measurements of cavity floor following carious dentin removal with Carisolv. Oper Dent, 28(4): 346–51, 2003.

29. Lager A, Thornqvist E, Ericson D. Cultivable bacteria in dentine after caries excavation using Rose-Bur or Carisolv. Caries Res, 37(3): 206–10, 2003.

30. Pinheiro SL, Aoki CMB, Mendes FM, Bengston NG, Bengston AL. Avaliação morfológica de dentina após diferentes métodos de remoção de tecido cariado. Rev Assoc Paul Cir Dent, 58(5): 363–8, 2004.

31. Cardoso PEC, Moura SK, Miranda Júnior WGM, Santos JFF, Tavares AU. Carisolv as an alternative method for caries removal. Revista da Postgraduacao, 11(2): 109–13, 2004.

32. Azrak B, Callaway A, Grundheber A, Stender E, Willershausen B. Comparison of the efficacy of chemomechanical caries removal (Cari-solvTM) with that of conventional excavation in reducing the cariogenic flora. Int J Paediatric Dent, 14(3): 182–91, 2004.

33. Calvo AFB, Rodrigues CRMD, Arana-Chaves VE. Tempo gasto para remoção de cárie em decíduos com métodos mecânico e químico-mecânico e aspecto da dentina em MEV [abstract Ia 074]. Braz Oral Res, 19: 54, 2005.

34. Yasuhiro T, Kousuke I, Yoshihiro N, Ishikawa K, Kazuomi S. Effect of phosphoric acid etching prior to self-etching primer application on adhesion of resin composite to enamel and dentin. Am J Dent, 15(5): 305–8, 2002.

35. Nakabayashi N. The hybrid layer. A resin dentin composite. Proc Finn Dent Soc, 88(1): 321–9, 1992.

36. Perdigão J. An ultra-morphological study of human dentine exposed to adhesive systems [Ph.D. Thesis]. Leuven: Catholic University of Leu-ven; 1995.

37. Salim DA. Estudo micromorfológico da interface entre um sistema adesivo de frasco único e a superfície desproteinizada da dentina de dentes decíduos [Dissertação de Mestrado]. São Paulo: Faculdade de Odontologia da USP; 2001

38. Nor JE, Feigal RJ, Dennison JB, Edwards CA. Dentin bonding: SEM comparison of the dentin surface in primary and permanent teeth. Pedi-atric Dent, 19(4): 246–57, 1997.

39. Koutsi V, Noonan RG, Horner JA, Simpson MD, Matthews WG, Pash-ley DH. The effect of dentin depth on the permeability and ultrastruc-ture of primary molars. Pediatric Dent, 16(1): 29–35, 1994.

40. Pashley DH. Denting bonding agents. Curr Opin Dent. 2:46-51, 1992.

41. Garberoglio R, Brannstrom M. Scanning electron microscopic investi-gation of human dentinal tubules. Arch Oral Biol, 21(6): 355–62, 1976.

42. Pashley D, Okabe A, Parham P. The relationship between dentin micro-hardness and tubule density. Endod Dent Traumatol, 1(5): 176–9, 1985.

43. Wolski K, Goldman M, Kronam J, Nathanson D. Dentinal bonding after chemomechanical caries removal effect of surface topography. Oper Dent, 14(4): 87–92, 1989.

44. Pinheiro SL, Santana AF, Oda M. Avaliação Morfológica da Interface de União dos Sistemas Adesivos em Dentes Decíduos. Rev Assoc Paul Cir Dent, 58(6): 429–34, 2004.

45. Sumikawa DA, Marshall GW, Gee L, Marshall SJ. Microstrucuture of primary tooth dentin. Pediatric Dent, 21(7): 439–44, 1999.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Scopus: CiteScore 2.0 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Submission Turnaround Time

Conferences

Top