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Open AccessDistribution of Streptococcus mutans and Streptococcus sobrinus in Saliva of Mexican Preschool Caries-free and Caries active Children by Microbial and Molecular (PCR) Assays
Distribution of Streptococcus mutans and Streptococcus sobrinus in Saliva of Mexican Preschool Caries-free and Caries active Children by Microbial and Molecular (PCR) Assays
- Juan Pablo Loyola-Rodriguez1,*,
- Rita Elizabeth Martinez-Martinez1
- Blanca Irma Flores-Ferreyra1
- Nuria Patiño-Marin1
- Angel Gabriel Alpuche-Solis1
- Juan Francisco Reyes-Macias1
1Dental Science with specialization in Advanced General Dentistry, San Luis Potosi University, Mexico
DOI: 10.17796/jcpd.32.2.cm00062530v856r4 Vol.32,Issue 2,December 2007 pp.121-126
Published: 01 December 2007
*Corresponding Author(s): Juan Pablo Loyola-Rodriguez E-mail: jloyola@uaslp.mx
Abstract
The aim of the study was to investigate the distribution of mutans streptococci (MS) infection of caries-free(CF) and caries-active (CA) preschool Mexican children by microbial and molecular assays. Eighty preschool children were divided into two groups, 40 CF and 40 CA children. Saliva samples were inoculated onto MSB to identify CFU and DNA extractions were tested by PCR. Our results indicated that there was no statistical difference (p>0.05) between groups either in age, weight, height or sex. S. sobrinus was detected by PCR twice as much in the CA group, the difference being statistically significant (p<0.05). dmfs index was positive correlated with S. mutans (r=0.2941, p=0.0081), S. sobrinus (r=0.3384, p=0.0021) and S. mutans-S. sobrinus (r=0.3978, p=0.0003). ANCOVA revealed that dmfs index had a significant effect on the distribution of CFU of S. mutans (p=0.0118) and S. sobrinus (p=0.03). When MSB was compared with PCR to identify MS, there was no statistical difference (p>0.05). We conclude that S. mutans and S. sobrinus were isolated in higher numbers from CA children and those harbouring both bacteria had higher dmfs scores. PCR is a useful tool in molecular epidemiology for dental caries studies; it was effective in detecting and identifying MS from saliva in children.
Keywords
Dental Caries; PCR; Streptococcus mutans; Streptococcus sobrinus; Saliva; Mexico
Cite and Share
Juan Pablo Loyola-Rodriguez,Rita Elizabeth Martinez-Martinez,Blanca Irma Flores-Ferreyra,Nuria Patiño-Marin,Angel Gabriel Alpuche-Solis,Juan Francisco Reyes-Macias. Distribution of Streptococcus mutans and Streptococcus sobrinus in Saliva of Mexican Preschool Caries-free and Caries active Children by Microbial and Molecular (PCR) Assays. Journal of Clinical Pediatric Dentistry. 2007. 32(2);121-126.
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