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Open AccessUse of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing
Use of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing
1Department of Pedodontics, Assistant Professor, Department of Pedodontics & Preventive Dentistry, Jaipur Dental College, Rajasthan University of Health Sciences, Rajasthan
2Department of Pedodontics, Yenepoya Dental College, Rajiv Gandhi University of Health Sciences, Karnataka
3Department of Oral & MaxilloFacial Surgery, Manipal College of Dental Sciences, Manipal
4Department of biochemistry, Yenepoya Dental College, Rajiv Gandhi University of Health Sciences, Karnataka
*Corresponding Author(s): Deepika.Pai E-mail: deepikamat@yahoo.com
Abstract
The aim of this in vitro study was to evaluate the remineralization of incipient enamel lesions by the topical application of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope.
Sixty caries free extracted teeth were used in the study. Forty teeth were used as test samples, ten as positive and ten as negative controls. The samples were demineralized and then remineralized by the topical application of CPP-ACP for a period of 14 days. The remineralization was evaluated with the use of laser fluorescence and scanning electron microscope (SEM). The results of this study showed that the laser fluorescent readings of test samples after remineralization were highly significant (p <0.001). A significant number of test samples observed under SEM showed high scores of remineralization.
Keywords
Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP), Remineralization, Laser fluorescence
Cite and Share
Deepika.Pai,Sham S Bhat,Abhay Taranath,Sharan Sargod,Vinita Pai M. Use of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing. Journal of Clinical Pediatric Dentistry. 2008. 32(3);201-206.
References
1. Nikiforuk G. Understanding dental caries, etiology and mechanisms, Basic and clinical aspects: The caries process-Morphological and chemical events. Karger Publications, 261–289, 1985.
2. de A.Silva MF, Burgerss RC, Sandham HJ, Jenkins GN. Effects of water soluble components of cheese on experimental caries in humans. J Dent Res, 66(1): 38–41, 1987.
3. Reynolds EC. Anticariogenic complexes of ACP stabilized by CPP: A review. Spec Care Dent, 18(1): 8–16, 1998.
4. Amjad Z, Koutsoukas PG, Nancollas GH. The mineralization of enamel surfaces. A constant composition kinetics study. J Dent Res, 60(10): 1783–1792, 1981.
5. Reynolds EC. Remineralization of enamel sub surface lesions by casein phosphopeptide stabilized calcium phosphate solutions. J Dent Res, 76(9): 1587–1595, 1997.
6. Exterkate RA, Damen JJ, TenCate JM. A single section model for enamel de and remineralization studies. J Dent Res, 72(12): 1599–603, 1993.
7. de Rooij JF, Nancollis GH. The formation and remineralization of artificial white spot lesions: A constant Compositional approach. J Dent Res, 63(6): 864–867, 1984.
8. Hosoya Y, Matsuzaka K, Inoue T, Marshall Jr GW. Influence of toothpolishing pastes and sealants on DIAGNOdent values. QuintessenceInt, 35: 605–611, 2004.
9. Hubbard MJ. Correlated light and scanning electron microscopy of artificial carious lesions. J Dent Res, 61(1): 14–19, 1982.
10. Holmen L, Thylstrup A, Ogaard B, Krgah F. A scanning electron microscopic study of progressive stages of enamel caries in vivo. Caries Res, 19: 355–367, 1985.
11. Shellis R P. A synthetic saliva for cultural studies of dental plaque. Arch Oral Biol 23: 485–489, 1978.
12. Gupta K, Tewari A, Sahni A, Chawla HS, Gauba K. Remineralizing efficacy of a mineral enriched mouth rinse and fluoridated dentifrice on artificial carious lesions: an in vivo scanning electron microscopic study. J Indian Soc Pedo Prev Dent, 16: 3: 67–71, 1998.
13. Renson CE. Changing patterns of dental caries. A survey of 20 countries. Ann Acad Med Singapore, 15: 284–298, 1986
14. Shaw JH, Ensfield BJ, Wollman DH. Studies on the relationship of dairy products to dental caries in caries-susceptible rats. J Nutr, 67: 253–273, 1959.
15. Lussi A, Megert B, Longbottom C, Reich E, Francesceut P. Clinical performance of a laser fluorescence device for detection of occlusal caries lesions. Eur J Oral Sci, 109: 14–19, 2001.
16. Jenkins GN, Ferguson DB. Milk and dental caries. Br Dent J 120: 472–477, 1966.
17. Weiss ME, Bibby BG. Effects of milk on enamel solubility. Arch OralBiol, 11: 49–57, 1966.
18. Reynolds EC, Cain CJ, Webber FL, Black CL, Riley P, Johnson IH, Perich JW. Anticariogenicity of calcium phosphate complexes of tryptic casein phosphopeptide in the rat. J Dent Res, 74(6): 1272–1279, 1995.
19. Papas AS, Joshi A, Belanger AJ, Kent RL, Palmer CA, DePaola PF. Dietary models for root caries. Am J Clin. Nutr, 61: 417S–422S, 1995.
20. Reynolds EC. Anticariogenic complexes of ACP stabilized by CPP. A review. Spec Care Dent, 18(1): 8–16, 1998.
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