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Original Research
Open AccessBiofilm Formation by Candida Species on Silicone Surfaces and Latex Pacifier Nipples: An in vitro Study
Biofilm Formation by Candida Species on Silicone Surfaces and Latex Pacifier Nipples: An in vitro Study
- Luiz Cezar da Silveira1
- Senda Charone1
- Lucianne Cople Maia1
- Rosangela Maria de Araújo Soares2
- Maristela Barbosa Portela1,2,*,
1Departamento de Odontopediatria e Ortodontia, Faculdade de Odontologia, Universidade Federal do Rio de Janeiro
2Departamento de Microbiologia Geral, Instituto de Microbiologia Professor Paulo de Góes (IMPPG), Universidade Federal do Rio de Janeiro
*Corresponding Author(s): Maristela Barbosa Portela E-mail: mbportela@hotmail.com
Abstract
The present study assessed the growth and development of biofilm formation by isolates of C. albicans, C. glabrata and C. parapsilosis on silicone and latex pacifier nipples. The silicone and latex surfaces were evaluated by scanning electronic microscopy (SEM). The plastic component of the nipple also seems to be an important factor regarding the biofilm formation by Candida spp. The biofilm growth was measured using the MTT reduction reaction. C. albicans was found to have a slightly greater capacity of forming biofilm compared to the other Candida species. Analysis of the pattern of biofilm development by C. albicans,C. glabrata and C. parapsilosis on latex and silicon pacifier shields showed an increased biofilm formation regarding the latter substrate. Silicone was shown to be more resistant to fungal colonization, particularly in the case of C. parapsilosis, despite the lack of any statistically significant differences (P > 0.05). In addition, silicone has a smoother surface compared to latex, whose surface was found to be rugose and irregular
Keywords
Candida, latex, silicone, pacifier, biofilm, babies, children
Cite and Share
Luiz Cezar da Silveira,Senda Charone,Lucianne Cople Maia,Rosangela Maria de Araújo Soares,Maristela Barbosa Portela. Biofilm Formation by Candida Species on Silicone Surfaces and Latex Pacifier Nipples: An in vitro Study. Journal of Clinical Pediatric Dentistry. 2009. 33(3);235-240.
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