Article Data

  • Views 712
  • Dowloads 144

Original Research

Open Access

Antibacterial Efficacy of Diode and Er:YAG Laser Irradiation in Experimentally Contaminated Primary Molar Root Canals

  • Senem Selvi Kuvvetli1,*,
  • Nuket Sandallı1
  • Nursen Topcuoglu2
  • Guven Kulekcı2

1Department of Pedodontics, Faculty of Dentistry, Yeditepe University

2Department of Microbiology, Faculty of Dentistry, Istanbul University

DOI: 10.17796/jcpd.34.1.n4172034x4054336 Vol.34,Issue 1,January 2010 pp.43-48

Published: 01 January 2010

*Corresponding Author(s): Senem Selvi Kuvvetli E-mail: sskuvvetli@yahoo.com.tr

Abstract

Objective: In vitro comparison of the antibacterial efficacy of Diode and Er:YAG laser irradiation with that of NaOCl irrigation in contaminated primary molar root canals. Study Design: 96 root canals prepared from 32 extracted primary molar teeth were mechanically enlarged and the teeth were randomly divided into 4 subgroups. The roots were inoculated with an overnight culture of Enterococcus faecalis in tryptic soy broth for 24 hours. The root canals irradiated with diode and Er:YAG laser and irrigated with NaOCl(5.25%) were experimental groups and untreated canals served as positive control group. Bacterial growth was analysed by counting viable E.faecalis on tryptic soy agar plates. Results: The number of bacteria was significantly reduced in experimental groups in comparison with the control group. Diode laser was determined to be more effective in reducing the number of bacteria when compared to Er:YAG laser. NaOCl irrigation was found significantly most effective. Conclusions: Diode laser irradiation and 5.25 % NaOCl application provided a significant antibacterial effect in vitro, in contaminated primary molar root canals.

Keywords

antibacterial effect, Er:YAG, diode, laser, primary molar, root canal

Cite and Share

Senem Selvi Kuvvetli,Nuket Sandallı,Nursen Topcuoglu,Guven Kulekcı. Antibacterial Efficacy of Diode and Er:YAG Laser Irradiation in Experimentally Contaminated Primary Molar Root Canals. Journal of Clinical Pediatric Dentistry. 2010. 34(1);43-48.

References

1. Fuks AB. Pulp therapy for the primary dentition. In: Pinkham JR, ed. Pediatric Dentistry Infancy Through Adolescence 3rd edn. W.B. Saunders Company, 341–55, 1999.

2. Camp JH. Pediatric Endodontic Treatment. Endodontic treatment fort he primary and young permanent dentition. In: Cohen S, Burns RC, eds. Pathways of the pulp 8th edn. St Louis, MO, USA: Mosby, 797–844, 1994.

3. Fuks AB, Eidelman E. Pulp therapy in the primary dentition. Curr Opin Dent, 1: 556–63, 1991.

4. Guideline on pulp therapy for primary and young permanent teeth. American Association of Pediatric Dentistry Reference Manual 2004–2005. Pediatr Dent, 7: 115–16, 2004.

5. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposure of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol, 20: 340–348, 1965.

6. Sundqvist G. Taxonomy, ecology, and pathogenicity of the root canal flora. Oral Surg 78: 522–530, 1994.

7. Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J, 30: 297–306, 1997.

8. Porteneier I, Waltimo TMT, Haapasalo M. Enterococcus faecalis – the root canal survivor and ‘star’ in post-treatment disease. Endodontic Topics, 6: 135–59, 2003.

9. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endondontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85: 86–93, 1998.

10. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J, 31: 1–7, 1998.

11. Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endondontic treatment in a North Am population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91: 579–586, 2001.

12. Siqueira JF, Lima KC, Magalhaes FAC, Lopes HP, de Uzeda M. Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod, 25: 332–335, 1999.

13. Barr ES, Kleier DJ, Barr NV. Use of nickel-titanium rotary files for root canal preparation in primary teeth. Pediatr Dent, 221: 77–8, 2000.

14. Seow WK. Comparison of ultrasonic and mechanical cleaning of primary root canals using a novel radiometric method. Pediatr Dent, 13: 136–41, 1991.

15. Silva LA, Leonardo MR, Nelson-Filho P, Tanomaru JM. Comparison of rotary and manual instrumentation techniques on cleaning capacity and instrumentation time in deciduous molars. J Dent Child, (Chic) 71: 45–7, 2004.

16. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res, 89: 321–328, 1981.

17. Byström A, Sundqvist G. Bacteriologic evaluation of the effect of 0.5%sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol, 55: 307–312, 1983.

18. Byström A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J, 18: 35–40, 1985.

19. Jeansonne MJ, White RR. A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antibacterial endodontic irrigants. J Endod, 20: 276–280, 1994.

20. Buck RA, Eleazer PD, Staat RH, Scheetz JP. Effectiveness of three endodontic irrigants at various tubular depths in human dentin. J Endod, 27: 206–208, 2001.

21. Gomes BPFA, Ferraz CC, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J, 34; 424–428, 2001.

22. Siqueira JF, Machado AG, Silveira RM, Lopes HP, de Uzeda M. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro. Int Endod J, 30: 279–282, 1997.

23. Siqueira JF, Rocas IN, Faviera A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1.0, 2.5, and 5.25% sodium hypochlorite. J Endod, 26: 331–334, 2000.

24. Radcliffe CE, Potouridou L, Qureshi R, et al. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms Actinomyces israelii, A naeslundii, Candida albicans, and Enterococcus faecalis. Int Endod J, 37: 438–446, 2004.

25. Goldman M, Goldman LB, Cavaleri R, Bogis J, Lin PS. The efficacy of several endodontic irrigating solutions: a scanning electron microscopic study: Part 2. J Endod, 8: 487–492, 1982.

26. Monika CM, Froner IC. A scanning electron microscopic evaluation of different root canal irrigation regimens. Braz Oral Res, 20: 235–240, 2006.

27. Le Goff A, Dautel-Morazin A, Guigand M, Vulcain JM, Bonnaure-Mallet M. An evaluation of the CO2 laser for endodontic disinfection. J Endod, 25: 105–108, 1999.

28. Piccolomini R, D’Arcangelo C, D’Ercole S, Catamo G, Schiaffino G, De Fazio P. Bacteriologic evaluation of the effect of Nd:YAG laser irradiation in experimental infected root canals. J Endod, 28: 276–278, 2002.

29. Folwaczny M, Mehl A, Jordan C, Hickel R. Antibacterial effects of pulsed Nd:YAG laser radiation at different energy settings in root canals. J Endod, 28: 24–29, 2002.

30. Eldeniz AU, Ozer F, Hadimli HH, Erganis O. Bactericidal efficacy of Er,Cr:YSGG laser irradiation against Enterococcus faecalis compared with NaOCl irrigation: an ex vivo pilot study. Int Endod J, 40: 112–9, 2007.

31. Moshonov J, Sion A, Kasirer J, Rotstein I, Stabholz A. Efficacy of argon laser irradiation in removing intracanal debris. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 79: 221–5, 1995.

32. Mehl A, Folwaczny M, Haffner C, Hickel R. Bactericidal effects of 2.94 microns Er:YAG-laser radiation in dental root canals. J Endod, 25: 490–3, 1999.

33. Moritz A, Gutknecht N, Schoop U, Goharkhay K, Doertbudak O, Sperr

W. Irradiation of infected root canals with a diode laser in vivo: results of microbiological examinations. Lasers Surg Med, 21: 221–6, 1997.

34. Gutknecht N, Franzen R, Schippers M, Lampert F. Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg, 22: 9–13, 2004.

35. Odabas ME, Bodur H, Baris E, Demir C. Clinical, radiographic, and histopathologic evaluation of Nd:YAG laser pulpotomy on human primary teeth. J Endod, 33: 415–21, 2007.

36. Liu JF. Effects of Nd:YAG laser pulpotomy on human primary molars. J Endod, 32: 404–7, 2006.

37. Huth KC, Paschos E, Hajek-Al-Khatar N, Hollweck R, Crispin A, Hickel R, Folwaczny M. Effectiveness of 4 pulpotomy techniques—randomized controlled trial. J Dent Res, 84: 1144–8, 2005.

38. Möller AJR. Microbiological examination of root canals and periapical tissues of human teeth: methodological studies. Scand Dent J, 74: 1–380, 1966

39. Haapasalo M, Ranta H, Ranta KT. Facultative gram-negative enteric rods in persistent periapical infections. Acta Odontol Scand, 41: 19–22, 1983.

40. Estrela C, Pimenta FC, Ito IY, Bammann LL. Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules. J Endod, 25: 416–8, 1999.

41. Haapasalo HK, Sirén EK, Waltimo TM, Ørstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J, 33: 126–31, 2000.

42. Cogulu D, Uzel A, Oncag O, Eronat C. PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2008 Sep;106(3): 443–9.

43. Schoop U, Moritz A, Kluger W, Patruta S, Goharkhay K, Sperr W, Wernisch J, Gattringer R, Mrass P, Georgopoulos A. The Er:YAG laser in endodontics: results of an in vitro study. Lasers Surg Med, 30: 360–364, 2002.

44. Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med, 35: 111–6, 2004.

45. Moritz A, Schoop U, Goharkhay K, Jakolitsch S, Kluger W, Wernisch J, Sperr W. The bactericidal effect of Nd:YAG, Ho:YAG, and Er:YAG laser irradiation in the root canal: an in vitro comparison. J Clin Laser Med Surg, 17: 161–4, 1999.

46. Seow WK. Comparison of ultrasonic and mechanical cleaning of primary root canals using a novel radiometric method. Pediatr Dent, 13: 136–41, 1991.

47. Barr ES, Kleier DJ, Barr NV. Use of nickel-titanium rotary files for root canal preparation in primary teeth. Pediatr Dent, 22: 77–78, 2000.

48. Canoglu H, Tekcicek MU, Cehreli ZC. Comparison of conventional, rotary, and ultrasonic preparation, different final irrigation regimens, and 2 sealers in primary molar root canal therapy. Pediatr Dent, 28: 518–23, 2006.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Scopus: CiteScore 2.0 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Submission Turnaround Time

Conferences

Top