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Original Research

Open Access

Staphylococcus Aureus Contamination in a Pediatric Dental Clinic

  • Thais de Cássia Negrini1
  • Cristiane Duque2,*,
  • Ana Carolina Mascarenhas de Oliveira3
  • Josimeri Hebling4
  • Luis C Spolidorio5
  • Denise MP Spolidorio4

1Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil

2Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil

3Department of Pediatric Dentistry, Federal Fluminense University (UFF), Nova Friburgo, Rio de Janeiro

4Department of Endodontics, Piracicaba School of Dentistry, State University of Campinas (Unicamp), Piracicaba, São Paulo, Brazil

5Department of Pediatric Dentistry, Araraquara School of Dentistry, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil

6Department of Physiology and Pathology, Araraquara School of Dentistry, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil

DOI: 10.17796/jcpd.34.1.n435k10291222035 Vol.34,Issue 1,January 2010 pp.13-18

Published: 01 January 2010

*Corresponding Author(s): Cristiane Duque E-mail: cristianeduque@yahoo.com.br

Abstract

Staphylococcus aureus strains can be disseminated during dental treatment and occasionally lead to contamination and infection of patients and dentists. The objective of this study was to determine the frequency and compare the number of S.aureus colonies isolated from the nose, hands and tongue of students and patients, as well as from the clinical environment, before and after dental treatment. Staphylococcus species were isolated from the tongue, nose and hands of 30 students and 30 patients and from the environment of a Pediatric Dentistry Clinic. The samples were incubated in SMA plates at 37o C for 48 hours. Results: The colonies that showed the presence of mannitol fermentation were collected as identification for Staphylococcus aureus, using CHROMagar and the coagulase test. The highest amount of S.aureus was found in the nose and tongue of children. In relation to dental students, more contamination was observed on gloved hands, followed by the tongue and hands without gloves, before clinical attendance. At the end of dental treatment, S. aureus colonies isolated from the gloved hands of students decreased significantly. Considering the clinical environment, the most contaminated areas were the auxiliary table and the storeroom, which was located at the center of the clinic. Conclusion: The dental clinic can be considered an environment for S. aureus cross-transmission. Preventative measures should be used to avoid the dissemination of pathogenic microorganisms.

Keywords

Staphylococcus aureus, Pediatric Dentistry, cross-contamination

Cite and Share

Thais de Cássia Negrini,Cristiane Duque,Ana Carolina Mascarenhas de Oliveira,Josimeri Hebling,Luis C Spolidorio,Denise MP Spolidorio. Staphylococcus Aureus Contamination in a Pediatric Dental Clinic. Journal of Clinical Pediatric Dentistry. 2010. 34(1);13-18.

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