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Original Research

Open Access

Identification of Cultivable Microorganisms from Primary Teeth with Necrotic Pulps

  • Gildardo Ledezma-Rasillo1
  • Hector Flores-Reyes2
  • Ana Ma. Gonzalez-Amaro2
  • Arturo Garrocho-Rangel1
  • Ma. del Socorro Ruiz-Rodriguez1
  • Amaury J Pozos-Guillen3

1Pediatric Dentistry Posgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México

2Endodontics Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México

3Pediatric Dentistry Postgraduate Program and Endodontics Postgraduate Program, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México

DOI: 10.17796/jcpd.34.4.20124lu111544377 Vol.34,Issue 4,July 2010 pp.329-334

Published: 01 July 2010

*Corresponding Author(s): Amaury J Pozos-Guillen E-mail: apozos@uaslp.mx

Abstract

The objective of this study was to identify cultivable microorganisms from primary teeth with necrotic pulps. This experimental study included 21 patients of both sexes between 4 and 7 years of age with necrotic pulps in primary teeth. Twenty-one maxillary and mandibular molars containing at least 1 necrotic canal, an abscess or sinus tract, one or more radiolucent areas in the furcation or periapical region, teeth having at least two thirds of root length, and carious lesions directly exposed to the oral environment were included. After antisepsis of the oral cavity, anesthesia of the affected tooth, and isolation and disinfection of the operative field, 3 sterile absorbent paper points were sequentially placed for 30 seconds for the collection of samples. The samples were immediately processed in an anaerobic chamber, and all isolated microorganisms were identified. Anaerobic species (anaerobic facultative and moderate anaerobes) were isolated in all root canals; 68.4% of root canal samples studied showed a polymicrobial nature. Most of the isolate consisted of Bifidobacterium Spp2 and Streptococcus intermedius. Other less frequently encountered species were Actinomyces israelii, Bifidobacterium spp 1, Clostridium spp, and Candida albicans. Results indicate the existence of combinations of bacterial species in root canal infections of the primary dentition with necrotic pulps, anaerobic bacteria predominating.

Keywords

anaerobic microorganisms, necrotic pulp, primary teeth

Cite and Share

Gildardo Ledezma-Rasillo,Hector Flores-Reyes,Ana Ma. Gonzalez-Amaro,Arturo Garrocho-Rangel,Ma. del Socorro Ruiz-Rodriguez,Amaury J Pozos-Guillen. Identification of Cultivable Microorganisms from Primary Teeth with Necrotic Pulps. Journal of Clinical Pediatric Dentistry. 2010. 34(4);329-334.

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