Article Menu

Export Article

More by Authors Links

Article Data

  • Views 1989
  • Dowloads 129

Original Research

Open Access

Dental Pulp Stem Cells from Primary Teeth Quality Analysis: Laboratory Procedures

  • Saha R1,*,
  • Tandon S2
  • Rajendran R3
  • Nayak R4

1Pedodontics & Preventive Dentistry, ITS-CDSR, Muradnagar

2Pedodontics & Preventive Dentistry, UP Dental College & Research Center

3Manipal, Stempeutics, Manipal

4Pedodontics and Preventive Dentistry, MCODS, Manipal

DOI: 10.17796/jcpd.36.2.6j0w230354732454 Vol.36,Issue 2,December 2011 pp.167-174

Published: 01 December 2011

*Corresponding Author(s): Saha R E-mail: rooposhi@yahoo.com

PDF (751.69 kB) View Full-text

Abstract

Objectives: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Materials and methods: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. Results: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Conclusion: Isolation of cells from oral tissues is technique sensitive.

Keywords

Stem cell, inflamed pulp, deciduous teeth, stem cell marker, in vitro differentiation

Cite and Share

Saha R,Tandon S,Rajendran R,Nayak R. Dental Pulp Stem Cells from Primary Teeth Quality Analysis: Laboratory Procedures. Journal of Clinical Pediatric Dentistry. 2011. 36(2);167-174.

URL:

https://www.jjocpd.com/articles/10.17796/jcpd.36.2.6j0w230354732454

References

1. Thomson  JA,  Itskovitz-Eldor  J,  Shapiro  S,  Waknitz  M,  Swiergiel  J, Marshall  V,  Jones  J  Embryonic  stem  cell  lines  derived  from  human blastocysts. Science 1282 (5391), 1145–7, 1998.

2. Gronthos  S,  Brahim  J,  Li  W,  Fisher  LW,  Cherman  N,  Boyde  A, DenBesten P, Robey PG, Shi S. Stem cell properties of human dental pulp stem cells. J Dent Res, 81, 531–535, 2002.

3. Yu J, Wang Y, Deng Z, Tang L, Li Y, Shi J, Jin Y; Odontogenic capa-bility: bone marrow stromal stem cells versus dental pulp stem cells. Biol Cell, Aug 99(8), 465–74, 2007.

4. Shobha Tandon,  Rooposhi  Saha,  Ramesh  Rajendran,  Rashmi  Nayak. Dental  Pulp  Stem  Cells  from  Primary  and  PermanentTeeth:  Quality-Analysis. J Clin Pediatr Dent, 35(1): 53–58, 2010.

5. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD,  Moorman  MA,  Simonetti  DW,  Craig  S,  Marshak  DR.  Multilin-neage potential of adult human mesenchymal stem cells. Science, Apr (2) 284, 143–147, 1999.

6. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA, 97, 13625–30, 2000.

7. Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM. Purifi-cation and ex vivo expansion of post natal human marrow mesodermal progenitor cells. Blood, 98, 2615–2625, 2001.

8. Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ. Molecular and cellular characterization of highly purified stromal stem cells derived from human bone marrow. J Cell Sci, 116, 1827–1835, 2003.

9. Suchanek  J,  Tomas  Soukup,  Benjamin  Visek,  Romana  Ivancakova, Lenka  Kucerova,  Jaroslav  Mokry.  Dental  pulp  stem  cells  and  their characterization; Biomed Pap Med Fac Univ Palacky Olomouc Czech Repu, 153(1): 31–36, 2009.

10. Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, Hockema JJ, Woods EJ, Goebel WS. Collection, cryopreservation, and characteriza-tion of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods, Jun 14(2), 149–56, 2008.

11. Bruder  SP,  Horowitz  MC,  Mosca  JD,  Haynesworth  SE.  Monoclonal antibodies reactive with human osteogenic cell surface antigens. Bone, Sep 21(3), 225–35, 1997.

12. Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirum-bolo G, Becchetti E, Marchionni C, Alviano F, Fossati V, Staffolani N, Franchina M, Grossi A, Bagnara GP. Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from den-tal pulp. Transplantation, 80, 836–842, 2005.

13. Niwa  H.  Molecular  mechanism  to  maintain  stem  cell  renewal  of  ES cells; Cell structure and function. 26, 137–148, 2001.

14. Zhou YY, Zeng FY. Two vital transcriptional factors Oct-4 and Nanog to keep the pluripotency and self-renewal of stem cells and related reg-ulation network. Yi Chuan, May 30(5), 529–36, 2008.

15. Dominici  M,  Le  Blanc  K,  Mueller  I,  Slaper  Cortenbach  I,  Marini  F, Krause D, Deans R, Keating A, Prockop D, Horwitz E. Minimal crite-ria for defining multipotent mesenchymal stromal cells. The interna-tional society for cellular therapy position statement. Cytotherap,y 8, 315–7, 2006.

Submission Turnaround Time

Top