Article Data

  • Views 913
  • Dowloads 149

Original Research

Open Access

Prevalence of Five Biofilm-Related Oral Streptococci Species from Plaque

  • Mitrakul K1,*,
  • Asvanund Y1
  • Vongsavan K1

1Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

DOI: 10.17796/jcpd.36.2.d7r750u227j85813 Vol.36,Issue 2,March 2012 pp.161-166

Published: 01 March 2012

*Corresponding Author(s): Mitrakul K E-mail: mkemthong@yahoo.com

Abstract

Objective: To examine the prevalence of five oral streptococci species of severe early childhood caries (S-ECC) and caries-free (CF) groups. Study design: Supra gingival plaque samples were obtained from 198 Thai children with ages ranging from one to six years old. Eighty-seven subjects had no caries (dmft=0), and 111 had S-ECC. After DNA extraction, S. mutans, S. sobrinus, S. sanguinis, S. oralis, and S. gordonii were identified by standard PCR using species-specific primers. Statistical analysis determined the differences among prevalence rates of each species using Pearson chi-square test. The relationship among dmft score, age, sex and caries status within each group was analyzed by logistical regression (p ≤ 0.05). Results: Sex was not correlated with any of the species detected in both groups (mean age =3.09, mean±SD of dmft=11.04±7.89). S. mutans was found at greatest prevalence in both groups followed by S. oralis. S. gordonii was detected at a high prevalence, but S. sobrinus and S. sanguinis were lower in S-ECC when compared with those from the CF group. Conclusion: S. mutans was associated significantly with S-ECC (p ≤ 0.05). Caries prevalence was highest (56.5%) in subjects infected by S. mutans alone. S. sanguinis prevalence was higher in the CF group, but not statiscally different. Infection with MS did not show higher caries prevalence.

Keywords

DNA, PCR, oral streptococci, plaque, mutans streptococci

Cite and Share

Mitrakul K,Asvanund Y,Vongsavan K. Prevalence of Five Biofilm-Related Oral Streptococci Species from Plaque. Journal of Clinical Pediatric Dentistry. 2012. 36(2);161-166.

References

1. The Sixth national dental health status survey. Dental Health Division, Ministry of Public Health, Thailand (2006–2007).

2. Thitasomakul S Piwat S, Thearmontree A et al. A longitudinal study of early childhood caries in 9- to 18-month-old Thai infants. Community Dent Oral Epidemiol 34: 429–436, 2006.

3. Acs G, Shulman R, Ng MW, Chussid S. The effect of dental rehabili-tation on the body weight of children with early childhood caries. Pedi-atr Dent, 21(2): 109–13, 1999.

4. Low W, Tan S, Schwartz S. The effect of severe caries on the quality of life in young children. Pediatr Dent, 21(6): 325–6, 1999.

5. Kolenbrander PE, London J. Adhere today, Adhere tomorrow: oral bac-terial adherence. J Bacteriol, 175(11): 3247–52, 1993.

6. Parisotto TM, Steiner-Oliveira C, Duque C, Peres RC, Rodrigues LK, Nobre-dos-Santos M. Relationship among microbiological composi-tion and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries. Arch Oral Biol, 55(5): 365–73, 2010.

7. Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol, 23(3): 196–205, 2008.

8. Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev, 44(2): 331–84, 1980.

9. Franco e Franco TC, Amoroso P, Marin JM, de Avila FA . Detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reac-tion. Braz Dent J, 18(4): 329–33, 2007.

10. Acevedo AM, Ray MV, Socorro M, Rojas-Sanchez F. Frequency and distribution of Mutans Streptococci in dental plaque from caries-free and caries-affected Venezuelan children. Acta Odontol Latinoam, 22: 15–20, 2009.

11. Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, et al. Longitu-dinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in preschool children. J Med Microbiol, 54: 661–665, 2005.

12. Linossier A, Gajardo M, Silva N et al. Prevalence of Streptococcus mutans in Pehuenche children, Chilean ethnic group. Rev Med Chil, 117: 872–878, 1989.

13. Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fuji-wara T. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis, 48(3): 195–9, 2004.

14. Okada M, Taniguchi Y, Hayashi F, Doi T, Suzuki J, Sugai M, et al. Late established mutans streptococci in children over 3 years old. Int J Dent, 60: 246–8, 2010.

15. Fujiwara T, Sasada E, Mima N, Ooshima T. Caries prevalence and sali-vary mutans streptococci in 0–2-year-old children of Japan. Commu-nity Dent Oral Epidemiol, 19(3): 151–4, 1991.

16. Tankkunnasombut S, Youcharoen K, Wisuttisak W, Vichayanrat S, Tiranathanagul S. Early colonization of mutans streptococci in 2- to 36-month-old Thai children. Pediatr Dent,31(1): 47–51, 2009.

17. Teanpaisan R, Thitasomakul S, Piwat S, Thearmontree A, Pithporn-chaiyakul W, Chankanka O. Longitudinal study of the presence of mutans streptococci and lactobacilli in relation to dental caries devel-opment in 3-to-24-month-old Thai children. Int Dent J, 57(6): 445–51, 2007.

18. Li Y, Saxena D, Barnes VM, Trivedi HM, Ge Y, Xu T. PCR-based dena-turing gradient gel electrophoresis in the evaluation of oral microbiota. Oral Microbiol Immunol, 21: 333–339, 2006.

19. Li Y, Ge Y, Saxena D, Caufield PW. Genetic profiling of the oral micro-biota associated with severe early childhood caries. J Clin Microbiol, 45(1): 81–71, 2007.

20. Becker MR, Pa17,ster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol, 40(3): 1001–9, 2002.

21. Munson MA, Banerjee A, Watson TF, Wade WG. Molecular analysis of the microflora associated with dental caries. J Clin Microbiol, 42(7): 3023–9, 2004.

22. Chhour KL, Nadkarni MA, Byun R, Martin FE, Jacques NA, Hunter N . Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol, 43(2): 843–9, 2005.

23. Sato T, Matsuyama J, Kumagai T et al. Nested PCR for detection of mutans streptococci in dental plaque. Lett Appl Microbiol, 37: 66–9, 2003.

24. Seki M, Yamashita Y, Shibata Y, Torigoe H, Tsuda H, Maeno M. Effect of mixed mutans streptococci colonization on caries development. Oral Microbiol Immunol, 21(1): 47–52, 2006.

25. Ge Y, Caufield PW, Fisch GS, Li Y. Streptococcus mutans and Strepto-coccus sanguinis colonization correlated with caries experience in chil-dren. Caries Res, 42(6): 444–8, 2008.

26. Kreth J, Zhang Y, Herzberg MC. Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interfer-ence with Streptococcus mutans. Journal of Bacteriology, 190: 4632–40, 2008.

27. Caufield PW, Cutter GR, Dasanayake AP. Initial acquisition of Mutans streptococci by infants: evidence for a discrete window of infectivity. J Dent Res, 72: 37–45, 1993.

28. Reference Manual. Definition of early childhood caries. American Academy of Pediatric Dentistry. 2009–2010.

29. Oho T, Yamashita Y, Shimazaki Y et al. Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction. Oral Microbiology and Immunology, 15: 258–262, 2000.

30. Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fuji-wara T. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis, 48(3): 195–9, 2004.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Scopus: CiteScore 1.8 (2023) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Submission Turnaround Time

Conferences

Top