Prevalence of Five Biofilm-Related Oral Streptococci Species from Plaque
1Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
DOI: 10.17796/jcpd.36.2.d7r750u227j85813 Vol.36,Issue 2,March 2012 pp.161-166
Published: 01 March 2012
*Corresponding Author(s): Mitrakul K E-mail: email@example.com
Objective: To examine the prevalence of five oral streptococci species of severe early childhood caries (S-ECC) and caries-free (CF) groups. Study design: Supra gingival plaque samples were obtained from 198 Thai children with ages ranging from one to six years old. Eighty-seven subjects had no caries (dmft=0), and 111 had S-ECC. After DNA extraction, S. mutans, S. sobrinus, S. sanguinis, S. oralis, and S. gordonii were identified by standard PCR using species-specific primers. Statistical analysis determined the differences among prevalence rates of each species using Pearson chi-square test. The relationship among dmft score, age, sex and caries status within each group was analyzed by logistical regression (p ≤ 0.05). Results: Sex was not correlated with any of the species detected in both groups (mean age =3.09, mean±SD of dmft=11.04±7.89). S. mutans was found at greatest prevalence in both groups followed by S. oralis. S. gordonii was detected at a high prevalence, but S. sobrinus and S. sanguinis were lower in S-ECC when compared with those from the CF group. Conclusion: S. mutans was associated significantly with S-ECC (p ≤ 0.05). Caries prevalence was highest (56.5%) in subjects infected by S. mutans alone. S. sanguinis prevalence was higher in the CF group, but not statiscally different. Infection with MS did not show higher caries prevalence.
DNA, PCR, oral streptococci, plaque, mutans streptococci
Mitrakul K,Asvanund Y,Vongsavan K. Prevalence of Five Biofilm-Related Oral Streptococci Species from Plaque. Journal of Clinical Pediatric Dentistry. 2012. 36(2);161-166.
1. The Sixth national dental health status survey. Dental Health Division, Ministry of Public Health, Thailand (2006–2007).
2. Thitasomakul S Piwat S, Thearmontree A et al. A longitudinal study of early childhood caries in 9- to 18-month-old Thai infants. Community Dent Oral Epidemiol 34: 429–436, 2006.
3. Acs G, Shulman R, Ng MW, Chussid S. The effect of dental rehabili-tation on the body weight of children with early childhood caries. Pedi-atr Dent, 21(2): 109–13, 1999.
4. Low W, Tan S, Schwartz S. The effect of severe caries on the quality of life in young children. Pediatr Dent, 21(6): 325–6, 1999.
5. Kolenbrander PE, London J. Adhere today, Adhere tomorrow: oral bac-terial adherence. J Bacteriol, 175(11): 3247–52, 1993.
6. Parisotto TM, Steiner-Oliveira C, Duque C, Peres RC, Rodrigues LK, Nobre-dos-Santos M. Relationship among microbiological composi-tion and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries. Arch Oral Biol, 55(5): 365–73, 2010.
7. Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol, 23(3): 196–205, 2008.
8. Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev, 44(2): 331–84, 1980.
9. Franco e Franco TC, Amoroso P, Marin JM, de Avila FA . Detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reac-tion. Braz Dent J, 18(4): 329–33, 2007.
10. Acevedo AM, Ray MV, Socorro M, Rojas-Sanchez F. Frequency and distribution of Mutans Streptococci in dental plaque from caries-free and caries-affected Venezuelan children. Acta Odontol Latinoam, 22: 15–20, 2009.
11. Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, et al. Longitu-dinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in preschool children. J Med Microbiol, 54: 661–665, 2005.
12. Linossier A, Gajardo M, Silva N et al. Prevalence of Streptococcus mutans in Pehuenche children, Chilean ethnic group. Rev Med Chil, 117: 872–878, 1989.
13. Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fuji-wara T. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis, 48(3): 195–9, 2004.
14. Okada M, Taniguchi Y, Hayashi F, Doi T, Suzuki J, Sugai M, et al. Late established mutans streptococci in children over 3 years old. Int J Dent, 60: 246–8, 2010.
15. Fujiwara T, Sasada E, Mima N, Ooshima T. Caries prevalence and sali-vary mutans streptococci in 0–2-year-old children of Japan. Commu-nity Dent Oral Epidemiol, 19(3): 151–4, 1991.
16. Tankkunnasombut S, Youcharoen K, Wisuttisak W, Vichayanrat S, Tiranathanagul S. Early colonization of mutans streptococci in 2- to 36-month-old Thai children. Pediatr Dent,31(1): 47–51, 2009.
17. Teanpaisan R, Thitasomakul S, Piwat S, Thearmontree A, Pithporn-chaiyakul W, Chankanka O. Longitudinal study of the presence of mutans streptococci and lactobacilli in relation to dental caries devel-opment in 3-to-24-month-old Thai children. Int Dent J, 57(6): 445–51, 2007.
18. Li Y, Saxena D, Barnes VM, Trivedi HM, Ge Y, Xu T. PCR-based dena-turing gradient gel electrophoresis in the evaluation of oral microbiota. Oral Microbiol Immunol, 21: 333–339, 2006.
19. Li Y, Ge Y, Saxena D, Caufield PW. Genetic profiling of the oral micro-biota associated with severe early childhood caries. J Clin Microbiol, 45(1): 81–71, 2007.
20. Becker MR, Pa17,ster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol, 40(3): 1001–9, 2002.
21. Munson MA, Banerjee A, Watson TF, Wade WG. Molecular analysis of the microflora associated with dental caries. J Clin Microbiol, 42(7): 3023–9, 2004.
22. Chhour KL, Nadkarni MA, Byun R, Martin FE, Jacques NA, Hunter N . Molecular analysis of microbial diversity in advanced caries. J Clin Microbiol, 43(2): 843–9, 2005.
23. Sato T, Matsuyama J, Kumagai T et al. Nested PCR for detection of mutans streptococci in dental plaque. Lett Appl Microbiol, 37: 66–9, 2003.
24. Seki M, Yamashita Y, Shibata Y, Torigoe H, Tsuda H, Maeno M. Effect of mixed mutans streptococci colonization on caries development. Oral Microbiol Immunol, 21(1): 47–52, 2006.
25. Ge Y, Caufield PW, Fisch GS, Li Y. Streptococcus mutans and Strepto-coccus sanguinis colonization correlated with caries experience in chil-dren. Caries Res, 42(6): 444–8, 2008.
26. Kreth J, Zhang Y, Herzberg MC. Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interfer-ence with Streptococcus mutans. Journal of Bacteriology, 190: 4632–40, 2008.
27. Caufield PW, Cutter GR, Dasanayake AP. Initial acquisition of Mutans streptococci by infants: evidence for a discrete window of infectivity. J Dent Res, 72: 37–45, 1993.
28. Reference Manual. Definition of early childhood caries. American Academy of Pediatric Dentistry. 2009–2010.
29. Oho T, Yamashita Y, Shimazaki Y et al. Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction. Oral Microbiology and Immunology, 15: 258–262, 2000.
30. Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fuji-wara T. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis, 48(3): 195–9, 2004.
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