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Original Research

Open Access

Aggregatibacter Actinomycetemcomitans in Down´s Syndrome Children

  • El Housseiny AA1,*,

1Faculty of Dentistry, King Abdulaziz University, Saudi Arabia. POBox: 80209 Jeddah 21589, Saudi Arabia.

DOI: 10.17796/jcpd.36.4.w805wm3r52474g24 Vol.36,Issue 4,July 2012 pp.417-421

Published: 01 July 2012

*Corresponding Author(s): El Housseiny AA E-mail: ahussini@hotmail.com

Abstract

To investigate the presence of Aggregatibacter actinomycetemcomitans (Aa) in Down’s syndrome (DS) children in comparison with age-matched normal children and to determine if Aa was acquired during childhood in DS individuals. Study design: 60 DS children and 60 healthy children participated in this cross-sectional study. Both groups were matched regarding gender and age and were equally selected from public schools/non-fee paying centers and private schools. Subgingival plaque sample were obtained and analyzed for Aggregatibacter actinomycetemcomitans (Aa). Traditional cultivation method was used on a selective medium “modified malachite green vancomycin agar.” Results: A significantly higher percentage of DS children had Aa in their subgingival plaque compared to their healthy counterparts (Chi square = 8.78, p=0.003). Although Aa was acquired by children as early as 5 years of age in both groups, no association was found between the occurrence of Aa and age. Conclusions: higher proportion of DS children acquired Aa compared to their healthy controls and Aa is acquired by children as early as 5 years of age.

Keywords

Aggregatibacter actinomycetemcomitans, Down’s syndrome, children, periodontal disease, prevalence.

Cite and Share

El Housseiny AA. Aggregatibacter Actinomycetemcomitans in Down´s Syndrome Children. Journal of Clinical Pediatric Dentistry. 2012. 36(4);417-421.

References

1. Hennequin M, Faulks D, Veyrune JL, Bourdiol P. Significance of oral health in persons with Down syndrome: a literature review. Dev Med Child Neurol, 41: 275–83, 1999.

2. Siffel C, Correa A, Cragan J, Alverson CJ. Prenatal diagnosis, pregnancy terminations and prevalence of Down syndrome in Atlanta. Birth Defects Res A Clin Mol Teratol, 70: 565–71, 2004.

3. Fernell E. Aetiological factors and prevalence of severe mental retardation in children in a Swedish municipality: the possible role of consanguinity. Dev Med Child Neurol, 40: 608–11, 1998.

4. Hoshi N, Hattori R, Hanatani K, Okuyama K, Yamada H, Kishida T, Yamada T, Sagawa T, Sumiyoshi Y, Fujimoto S. Recent trends in the prevalence of Down syndrome in Japan, 1980-1997. Am J Med Genet, 84: 340–5, 1999.

5. Abdel-Fattah S, Shawky RM, Fattah SA, Kashef N. Congenital anomalies prevalent among Egyptian Mongols. Egyptian J Paediatr, 8: 367–75, 1991.

6. Seagriff-Curtin P, Pugliese S, Romer M. Dental considerations for individuals with Down syndrome. N Y State Dent J, 72: 33–5, 2006.

7. Modeer T, Barr M, Dahllöf G. Periodontal disease in children with Down Syndrome. Scand J Dent Res, 98: 228–34, 1990.

8. Zigmond M, Stabholz A, Shapira J, Chaushu G, Becker A, Yefenof E, Merrick J, Chaushu S. The outcome of a preventive dental care programme on the prevalence of localized aggressive periodontitis in Down’s syndrome individuals. J Intellect Disabil Res, 50: 429–500, 2006.

9. Reuland-Bosma W, van den Barselaar MT, van de Gevel JS, Leijh PC, de Vries-Huiges H, The HT. Nonspecific and specific immune responses in a child with Down’s syndrome and her sibling. A case report. J Periodontol, 59: 249–53, 1988.

10. Loureiro A, Costa F, da Costa J. The impact of periodontal disease on the quality of life of individuals with Down syndrome. Downs Syndr Res Pract, 12: 50–4, 2007.

11. Saxén L, Aula S, Westermarck T. Periodontal disease associated with Down’s syndrome: an orthopantomographic evaluation. J Periodontol, 48: 337–40, 1977.

12. Saxén L, Aula S. Periodontal bone loss in patients with Down’s syndrome: a follow-up study. J Periodontol, 53: 158–62, 1982.

13. Christersson LA. Actinobacillus actinomycetemcomitans and localized juvenile periodontitis. Clinical, microbiologic and histologic studies. Swed Dent J Suppl, 90: 1–46, 1993.

14. Calabrese N, Galgut P, Mordan N. Identification of Actinobacillus actinomycetemcomitans, Treponema denticola and Porphyromonas gingivalis within human dental calculus: a pilot investigation. J Int Acad Periodontol, 9: 118–28, 2007.

15. Sanz M, Lau L, Herrera D, Morillo JM, Silva A. Methods of detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythensis in periodontal microbiology, with special emphasis on advanced molecular techniques: a review. J Clin Periodontol, 31: 1034–47, 2004.

16. Alsina M, Olle E, Frias J. Improved, low-cost selective culture medium for Actinobacillus actinomycetemcomitans. J Clin Microbiol, 39: 509–13, 2001.

17. Boutaga K, Savelkoul PH, Winkel EG, van Winkelhoff AJ. Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction. J Periodontol, 78: 79–86, 2007.

18. Balashova NV, Park DH, Patel JK, Figurski DH, Kachlany SC. Interaction between leukotoxin and Cu, Zn superoxide dismutase in Aggregatibacter actinomycetemcomitans. Infect Immun, 75: 4490–7, 2007.

19. Amano A, Kishima T, Kimura S, Takiguchi M, Ooshima T, Hamada S, Morisaki I. Periodontopathic bacteria in children with Down syndrome. J Periodontol, 71: 249–55, 2000.

20. American Dental Association and American Academy of Periodontology. Periodontal Screening and Recording Training Program Kit, Chicago, 1992.

21. Jervøe-Storm PM, Alahdab H, Koltzscher M, Fimmers R, Jepsen S. Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. J Periodontol, 78: 909–17, 2007.

22. Sakellari D, Arapostathis KN, Konstantinidis A. Periodontal conditions and subgingival microflora in Down syndrome patients. A case-control study. J Clin Periodontol, 32: 684–90, 2005.

23. Taubert KA, Dajani AS. Preventing bacterial endocarditis: American Heart Association guidelines. Am Fam Physician, 57: 457–68, 1998.

24. Sakamoto M, Takeuchi Y, Umeda M, Ishikawa I, Benno Y. Rapid detection and quantification of five periodontopathogenic bacteria by real-time PCR. Microbiol Immunol, 45: 39–44, 2001.

25. Müller HP, Heinecke A, Fuhrmann A, Eger T, Zoller L. Intraoral distribution of Actinobacillus actinomycetemcomitans in young adults with minimal periodontal disease. J Periodontal Res, 36: 114–23, 2001.

26. Cortelli SC, Cortelli JR, Jorge AOC. Relationship among clinical parameters, smoking, and detection of Actinobacillus actinomycetemcomitans (in Portuguese). Revista Odontol UNESP, 30: 201–14, 2001.

27. Horz HP, Conrads G. Diagnosis and anti-infective therapy of periodontitis. Expert Rev Anti Infect Ther, 5: 703–15, 2007.

28. Gabre P, Martinsson T, Gahnberg L. Longitudinal study of dental caries, tooth mortality and interproximal bone loss in adults with intellectual disability. Eur J Oral Sci, 109: 20–6, 2001.

29. Ledder RG, Gilbert P, Huws SA et al. Molecular analysis of the suhgingival microbiota in health and disease. Appl Environ Microbiol, 73: 516–23, 2007.

30. Barr-Agholme M, Dahllöf G, Linder L, Modeer T. Actinobacillus actinomycetemcomitans, Capnocytophaga and Porphyromonas gingivalis in subgingival plaque of adolescents with Down syndrome. Oral Microbiol Immunol, 7: 244–8, 1992.

31. Sreedevi H, Munshi AK. Neutrophil chemotaxis in Down syndrome and normal children to Actinobacillus actinomycetemcomitans. J Clin Pediatr Dent, 22: 141–6, 1998.

32. Okada M, Hayashi F, Soda Y, Zhong X, Miura K, Kozai K. Intra-familial distribution of nine putative periodontopathogens in dental plaque samples analyzed by PCR. J Oral Sci, 46: 149–56, 2004.

33. Kulekci G, Leblebicioglu B, Keskin F, Ciftci S, Badur S. Salivary detection of periodontopathic bacteria in periodontally healthy children. Anaerobe, 14: 49–54, 2008.

34. Oredugba FA. Oral health condition and treatment needs of a group of Nigerian individuals with Down syndrome. Downs Syndr Res Pract, 12: 72–6, 2007.

35. Sakellari D, Belibasakis G, Chadjipadelis T, Arapostathis K, Konstantinidis A. Supragingival and subgingival microbiota of adult patients with Down’s syndrome. Changes after periodontal treatment. Oral Microbiol Immunol, 16: 376–82, 2001.

36. Fine DH, Markowitz K, Furgang D et al.. Aggregatibacter actinomycetemcomitans and its relationship to initiation of localized aggressive periodontitis: longitudinal cohort study of initially healthy adolescents. J Clin Microbiol, 45: 3859–69, 2007.

37. Deng T, Wang L, Lv J et al. Association of three bacterial species and periodontal status in chinese adults: an epidemiological approach. J Clin Microbiol, 49: 184–8, 2011.

38. Gafan GP, Lucas VS, Roberts GJ et al . Prevalence of periodontal pathogens in dental plaque of children. J Clin Microbiol, 42: 4141–6, 2004.

39. Riep B, Edesi-Neub L, Claessen F et al. Are Putative Periodontal Pathogens Reliable Diagnostic Markers? J Clin Microbiol, 47: 1705–11, 2009.

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