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Original Research

Open Access

Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth

  • Topcuoglu N1,*,
  • Bozdoğan E2
  • Aktoren O2
  • Kulekci G1

1Department of Microbiology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey

2Department of Paediatric Dentistry. Faculty of Dentistry, Istanbul University, Istanbul, Turkey

3,

DOI: 10.17796/jcpd.38.2.5252712533082gt0 Vol.38,Issue 2,March 2014 pp.155-160

Published: 01 March 2014

*Corresponding Author(s): Topcuoglu N E-mail: nursentopcuoglu@yahoo.com

Abstract

Objective: Knowledge of the microbial composition of deciduous endodontic infections is limited. This study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. Study Design: The samples were collected from 30 root canals of primary teeth with primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (Parocheck®). Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequently isolated bacterium (96.7%), followed by Prevotella intermedia (86.7%), Parvimonas micra (83.3%), Treponema denticola (76.7%) and Tannerella forsythia (66.7%). These bacteria were also present in high levels. All pairs of bacterial species were positively associated (RR≯1), except P.intermedia and P.micra. On average, five species (range:3-8) were detected per amplified sample. Root canals of teeth with ≯5 different species were statistically associated with periapical radiolucency (P=0.049). Conclusions: Primary teeth with endodontic infections show a highly diverse variety of bacteria, in which the most prevalent specie are present in high proportions. The well-directed use of the improved microarray technology will provide additional valuable information for causative factors associated with endodontic diseases, helping to develop more successful antibacterial or anti-inflammatory treatment strategies.

Keywords

endodontic infections, primary teeth, microarray analysis, oral bacteria

Cite and Share

Topcuoglu N,Bozdoğan E,Aktoren O,Kulekci G. Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth. Journal of Clinical Pediatric Dentistry. 2014. 38(2);155-160.

References

1. Siqueira JF Jr. Endodontic infections: concepts, paradigms and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94: 281-293, 2002.

2. Svensater G, Bergenholtz G. Biofilms in endodontic infections. Endod Topics 9: 27-36, 2004.

3. Sundqvist G. Ecology of the root canal flora. J Endod 18:427-430, 1992.

4. Siqueira JF Jr, Roças IN, Souto R, Uzeda M, Colombo AP. Checkerboard DNA–DNA hybridization analysis of endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 89: 744–748, 2000.

5. Rolph HJ, Lennon A, Riggio MP et al. Molecular identification of micro-organisms from endodontic infections. J Clin Microbiol 39: 3282–3289, 2001.

6. Munson MA, Pitt Ford T, Chong B, Weightman A, Wade WG. Molecular and cultural analysis of the microflora associated with endodontic infec-tions. J Dent Res 81: 761–766, 2002.

7. Brito LC, Teles FR, Teles RP et al. Use of multiple displacement amplifica-tion and checkerboard DNA–DNA hybridization to examine the microbiota of endodontic infections. J Clin Microbiol 45: 3039–3049, 2007.

8. Sassone L, Fidel R, Figueiredo L, Fidel S, Faveri M, Feres M. Evaluation of the microbiota of primary endodontic infections using checkerboard DNA–DNA hybridization. Oral Microbiol Immunol 22: 390–397, 2007.

9. Marsh SJ, Largent MD. A bacteriological study of the pulp canals of infected primary molars. J Dent Child 34: 460-470, 1967.

10. Toyoshima Y, Fukushima H, Inoue JI et al. A bacteriological study of peri-apical pathosis on deciduous teeth. JPN Dent J 26: 44, 1988.

11. Pazelli LC, Freitas AC, Ito IY, Souza-Gugelmin MC, Medeiros AS, Nelson-Filho P. Prevalence of microorganisms in root canals of human deciduous teeth with necrotic pulp and chronic periapical lesions. Pesqui Odontol Bras 17: 367–371, 2003.

12. Ruviere DB, Leonardo MR, da Silva LA, Ito IY, Nelson-Filho P. Assess-ment of the microbiota in root canals of human primary teeth by checker-board DNA–DNA hybridization. J Dent Child (Chic) 74: 118–123, 2007.

13. Cogulu D, Uzel A, Oncag O, Eronat C. PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106: 443–449, 2008.

14. Tavares WL, Neves de Brito LC, Teles RP et al. Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization. Int Endod J 44: 225-235, 2011.

15. Vianna ME, Horz HP, Gomes BP, Conrads G. Microarrays complement culture methods for identification of bacteria in endodontic infections. Oral Microbiol Immunol 20: 253–258, 2005.

16. Siqueira JF Jr. Taxonomic changes of bacteria associated with endodontic infections. J Endod 29: 619–623, 2003.

17. Külekci G, Ciftci S, Keskin F, Kilic AO, Türkoglu S, Badur S, et al. PCR analysis of Actinobacillus actinomycetemcomitans, Porphyromonas gingi-valis, Treponema denticola and Fusobacterium nucleatum in middle ear effusion. Anaerobe 7:241-246, 2001.

18. Cohen MM, Joress SM, Calisti LP, Mass B. Bacteriologic study of infected deciduous molars. Oral Surg Oral Med Oral Pathol 3: 1382-1386, 1960.

19. Tomic-Karovic K, Jelinek E. Comparative study of the bacterial flora in the surroundings, the root canals and sockets of deciduous molars. Int Dent J 21: 375-388, 1971.

20. Siqueira JF Jr, Roças IN. Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol 43: 3314–3319, 2005.

21. Siqueira JF Jr, Roças IN, Alves FR, Santos KR. Selected endodontic patho-gens in the apical third of infected root canals: a molecular investigation. J Endod 30: 638–643, 2004.

22. de Souza CA, Teles RP, Souto R, Chaves MA, Colombo AP. Endodontic therapy associated with calcium hydroxide as an intracanal dressing: microbiologic evaluation by the checkerboard DNA–DNA hybridization technique. J Endod 31: 79–83, 2005.

23. Suzuki MT, Giovannoni SJ. Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Micro-biol 62: 625–630, 1996.

24. Ito IY, Junior FM, Paula-Silva FW, Da Silva LA, Leonardo MR, Nelson-Filho P. Microbial culture and checkerboard DNA-DNA hybridization assessment of bacteria in root canals of primary teeth pre- and post-end-odontic therapy with a calcium hydroxide/chlorhexidine paste. Int J Paediatr Dent 21: 353-360, 2011.

25. Siqueira JF Jr, Roças IN. Community as the unit of pathogenicity: an emerging concept as to the microbial pathogenesis of apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107: 870-878, 2009.

26. Frias J, Olle E, Alsina M. Periodontal pathogens produce quorum sensing signal molecules. Infect Immun 69: 3431-3434, 2001.

27. Papapanou PN. Population studies of microbial ecology in periodontal health and disease. Ann Periodontol 7: 54–61, 2002.

28. Gomes BP, Pinheiro ET, Gade-Neto CR et al. Microbiological examination of infected dental root canals. Oral Microbiol Immunol 19: 71–76, 2004.

29. Roças IN, Siqueira JF Jr. Root canal microbiota of teeth with chronic apical periodontitis. J Clin Microbiol 46: 3599-3606, 2008.

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