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Original Research

Open Access

Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth

  • Topcuoglu N1,*,
  • Bozdoğan E2
  • Aktoren O2
  • Kulekci G1

1Department of Microbiology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey

2Department of Paediatric Dentistry. Faculty of Dentistry, Istanbul University, Istanbul, Turkey

3,

DOI: 10.17796/jcpd.38.2.5252712533082gt0 Vol.38,Issue 2,March 2014 pp.155-160

Published: 01 March 2014

*Corresponding Author(s): Topcuoglu N E-mail: nursentopcuoglu@yahoo.com

Abstract

Objective: Knowledge of the microbial composition of deciduous endodontic infections is limited. This study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. Study Design: The samples were collected from 30 root canals of primary teeth with primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (Parocheck®). Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequently isolated bacterium (96.7%), followed by Prevotella intermedia (86.7%), Parvimonas micra (83.3%), Treponema denticola (76.7%) and Tannerella forsythia (66.7%). These bacteria were also present in high levels. All pairs of bacterial species were positively associated (RR≯1), except P.intermedia and P.micra. On average, five species (range:3-8) were detected per amplified sample. Root canals of teeth with ≯5 different species were statistically associated with periapical radiolucency (P=0.049). Conclusions: Primary teeth with endodontic infections show a highly diverse variety of bacteria, in which the most prevalent specie are present in high proportions. The well-directed use of the improved microarray technology will provide additional valuable information for causative factors associated with endodontic diseases, helping to develop more successful antibacterial or anti-inflammatory treatment strategies.

Keywords

endodontic infections, primary teeth, microarray analysis, oral bacteria

Cite and Share

Topcuoglu N,Bozdoğan E,Aktoren O,Kulekci G. Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth. Journal of Clinical Pediatric Dentistry. 2014. 38(2);155-160.

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