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Original Research
Open AccessTreatment of Oral Ulcers in Dogs Using Adipose Tissue-Derived
Mesenchymal Stem Cells
Treatment of Oral Ulcers in Dogs Using Adipose Tissue-Derived Mesenchymal Stem Cells
1Faculty of Dentistry, Preventive Dental Sciences Department, Pediatric Dentistry Division, Jeddah, Kingdom of Saudi Arabia
2 Faculty of Dentistry, Preventive Dental Sciences Department, Pediatric Dentistry Division, Jeddah, Kingdom of Saudi Arabia and Lecturer at El Azhar University, Faculty of Dental Medicine for Girls, Pedodontic Department, Cairo, Egypt.
DOI: 10.17796/jcpd.38.3.193115427jg6vl60 Vol.38,Issue 3,April 2014 pp.215-222
Published: 01 April 2014
*Corresponding Author(s): Alamoudi N M E-mail: nalamoudi2011@gmail.com nalamoudi@kau edu sa
Abstract
Adipose tissue Derived Mesenchymal Stem cells (ADMSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. The goal of this study was to investigate the effects of ADMSCs transplantation on oral ulcer healing in dogs. Study design: Mesenchymal stem cells were isolated from adipose tissues of dogs obtained by suction-assisted lipectomy (liposuction), by dish adherence and were expanded in culture. Oral ulcers were induced by topical application of formocresol in the oral cavity of 18 dogs. The dogs were classified into 3 groups. Either autologous ADMSCs, Corticosteriod (Dexamethasone) or vehicle (saline) was injected. The healing process of the ulcer was monitored histopathologically. Gene expression of vascular endothelial growth factor (VEGF), platelets derived growth factor (PDGF), epidermal growth factor (EGF) and collagen was assessed in biopsies obtained from all ulcers ‘’as healing markers’’, by real time polymerase chain reaction (PCR). Results: ADMSCs group showed significantly accelerated oral ulcer healing compared with the Dexamethasone and control groups. There was increased expression of VEGF, PDGF, EGF and collagen genes in ADMSCs-treated ulcers compared with Dexamethasone and controls. Conclusion: ADMSCs transplantation may help accelerate oral ulcer healing, possibly through the induction of angiogenesis by VEGF and PDGF, as well as epithelial and connective tissue proliferation as evidenced by increased EGF and collagen gene expression.
Keywords
Adipose tissue- Derived mesenchymal stem cells, corticosteroid, oral ulcer, dogs.
Cite and Share
Alamoudi N M,El Ashiry E A,Farsi N M,El Derwi D A,Atta H M. Treatment of Oral Ulcers in Dogs Using Adipose Tissue-Derived Mesenchymal Stem Cells. Journal of Clinical Pediatric Dentistry. 2014. 38(3);215-222.
References
1. Field EA, Longman LP. Tyldesley’s Oral Medicine, 5th edn. Oxford: Oxford University Press, 2003.
2. Porter SR, Scully C. Adverse drug reactions in the mouth. Clin Dermatol;18: 525–532. 2000.
3. Francis B, Quinn F, Matthew W. Ulcerative lesions of the oral cavity. Grand Rounds Presentation, UTMB, Department of Otolaryngology, TX: 2002.
4. Field EA, Allan RB. Review article: Oral ulceration–aetiopathogenesis, clinical diagnosis and management in the gastrointestinal clinic. Aliment Pharmacol Ther; 18: 949–962. 2003.
5. Scully C, Gorsky M, Lozada-Nur F. The diagnosis and management of recurrent aphthous stomatitis: a consensus approach. J Am Dent Assoc;134:200–207. 2003.
6. Fernandez J, Escorsell A, Zabalza M, Felipe V, Navasa M, Mas A, Lacy A, Ginès P, Arroyo V. Adrenal insufficiency in patients with cirrhosis and septic shock: Effect of treatment with hydrocortisone on survival. Hepatology;44:1288–1295. 2006.
7. Porter SR, Scully C, Pedersen A. Recurrent aphthous stomatitis; Crit Rev Oral Biol Med.; 9: 306–321. 1998.
8. Tarnawski A. Cellular and molecular mechanisms of ulcer healing lesions from gastrointestinal ulcers. Dig Dis Sci; 50(Suppl. 1):S24–33. Review. 2005
9. Tarnawski A S, Ahluwalia A. Molecular mechanisms of epithelial regeneration and neovascularization during healing of gastric and esophageal ulcers. Curr Med Chem.;19(1):16-27. 2012.
10. Davidson J. Growth factors in wound healing. Wounds.;7(suppl A):53A–64A. 1995.
11. MacArthur BD, Oreffo ROC. Bridging the gap. Nature. 2005; 433(7021):19.
12. Gronthos S, Zannettino ACW. A method to isolate and purify human bone marrow stromal stem cells. In: Mesenchymal stem cells: methods and protocols. Prockop DJ, Bunnell BA, Phinney DG, editors. Totowa, NJ: Humana Press, pp. 45-57, 2008.
13. National Institutes of Health Resource for Stem Cell Research. The stem cell in formation Stem Cell Basics page. Http://stemcells.nih.gov/infbasics/ defaultpage.asp. Accessed July 21, 2008.
14. Granero-Molto F, Weis K, Miga M, Landis B, Myers T, O’Rear L, Longobardi L, Jansen D, Mortlock M, Spagnoli A. Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells;27:1887- 1898. 2009.
15. El-Menoufy H, Aly LA, Aziz MT, et al. The role of bone marrow-derived mesenchymal stem cells in treating formocresol induced oral ulcers in dogs. J Oral Pathol Med.;39(4):281-9. 2010.
16. Prockop DJ. Marrow stromal cells as stem cells for non hematopoietic tissues. Science.;276:71-74. 1997.
17. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simoneti DW, Craig S, AMrshak DR. Multilineage potential of adult human mesenchymal stem cells. Science;284:143-147. 1999.
18. Stan Gronthos S, Zannettino A, Hay S, Shi S, Graves S, Kortesidis A, Simmons J. Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci;116:1827- 1835. 2003.
19. Mareschi K, Biasin E, Piacibello W, Aglietta M, Madon E, Fagioli F. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica; 86:1099-1100. 2001.
20. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell W, Katz AJ, Penhaim P, Lorenz P, Hedrick P. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng;7:211-228. 2001.
21. Mizuno H, Zuk PA, Zhu M, Lorenz HP, Benhaim P, Hedrick MH. Myogenic differentiation by human processed lipo aspirate cells. Plast Reconstr Surg;109:199-209. 2002.
22. Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells;24:1294-1301. 2006.
23. Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy;6:7-14. 2004.
24. Rodriguez AM, Elabd C, Amri EZ, Ailhaud G, Dani C. The human adipose tissue is a source of multipotent stem cells. Biochimie; 87:125-128. 2005.
25. Tomiyama K, Murase N, Stolz DB, Toyokawa H, O’Donnell DR, Smith DM, Dudas JR, Rubin JP, Marra KG. Characterization of transplanted green fluorescent protein+ bone marrow cells into adipose tissue. Stem Cells; 26:330-338.2008.
26. Yamazoe K, Mishima H, Torigoe K, Iijima H, Watanabe K, Sakai H, Kudo T. Effects of atelocollagen gel containing bone marrow-derived stromal cells on repair of osteochondral defect in a dog. J Vet Med Sci; 69:835-839. 2007.
27. Jaiswal N, Haynesworth S, Caplan A, Bruder S. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem; 64: 295–312. 1997.
28. Hou L, Cao H, Wang D, et al. Induction of umbilical cord blood mesenchymal stem cells into neuron-like cells in vitro. Int J Hematol; 78: 256–61. 2003.
29. Kenneth J. Livak and Thomas D. Schmittgen: Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 22DDCT. Methods; 25:402–408. 2001.
30. Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis SJ. Multi-organ, multi-lineage engraftment by a single bone marrow derived stem cell. Cell;105: 369–377. 2001.
31. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell;13(12): 4279-4295. 2002.
32. Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM, Bunnell BA. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem;99:1285-1297. 2006.
33. Boeve C, Dermaut L. Formocresol pulpotomy in primary molars: a longterm radiographic evaluation. ASDC J Dent Child;49:191–6. 1982.
34. AnaTeresa S, Luis C, Lizeti T, Oliveira R. Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice. Braz Dent J;19: 33–39. 2008.
35. Yamasaki M, Nakamura H, Kameyama Y. Irritating effect of formocresol after pulpectomy in vivo. Int Endod J; 27:245–251. 1994.
36. Neels JG, Thinnes T, Loskutoff DJ. Angiogenesis in an in vivo model of adipose tissue development. Faseb J; 18:983-985. 2004.
37. Charrière G, Cousin B, Arnaud E, André M, Bacou F, Penicaud L, Casteilla L. Preadipocyte conversion to macrophage. Evidence of plasticity. J Biol Chem.;278:9850-9855. 2003.
38. Guidry JA, George JN, Vesely SK, Kennison SM, Terrell DR. Corticosteroid side-effects and risk for bleeding in immune thrombocytopenic purpura: patient and hematologist perspectives 2009. John Wiley & Sons A/S European Journal of Haematology 83:175–182.
39. Stanbury RM, Graham EM. Systemic corticosteroid therapy-side effects and their management. br j ophthalmol;82:704–708. 1998.
40. Suzuki B, Peterson D, Siegel M. Medications influencing immune response and wound healing. Grand Rounds Oral-Sys Med; 2:159–168. 2007.
41. Moraes LA, Paul-Clark MJ, Rickman A, Flower RJ, Goulding NJ, Perretti M. Ligand-specific glucocorticoid receptor activation in human platelets. Blood; 106:4167-4175. 2005.
42. Gerritsen M, Tomlinson J, Zlot C, Ziman M, Hwang S. Using gene expression profiling to identify the molecular basis of the synergisticactions of hepatocyte growth factor and vascular endothelial growth factor in human endothelial cells. Br J Pharmacol;140: 595–610. 2003.
43. Sabine W, Richard G. Regulation of Wound Healing by Growth Factors and Cytokines. Physiol Rev;83:835-870. 2003.
44. Herbst RS. Review of epidermal growth factor receptor biology. Int J Radiation Oncology Biol Phys;59(2):21–26. 2004.
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