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Original Research

Open Access

Effect of some industrialized acidic beverages on the roughness of pit and fissure sealants: an in vitro study

  • Georgina Baca-Solano1
  • Rosalía Contreras-Bulnes1,*,
  • Laura Emma Rodríguez-Vilchis1
  • Bernardo Teutle-Coyotecatl1
  • Ulises Velazquez-Enriquez1

1Center for Research and Advanced Studies in Dentistry, School of Dentistry, Autonomous University of Mexico State, 50130 Toluca, Mexico State, Mexico

DOI: 10.22514/jocpd.2022.031

Submitted: 27 July 2022 Accepted: 29 September 2022

Online publish date: 22 November 2022

*Corresponding Author(s): Rosalía Contreras-Bulnes E-mail:


This study evaluated in vitro the roughness of the pit and fissure sealants (PFS) after immersion in some industrialized acidic beverages. 120 discs (5 mm diameter and 2 mm thick) of 4 commercial brands (3M, Ivoclar, Ultradent, and VOCO) were immersed in 1.5 mL of 3 different industrialized acid beverages (soft drink, apple juice, and fermented milk) and incubated at 37 ºC for 15 and 30 days. Surface roughness (Ra and Rz) was measured at the beginning (0), 15 (1), and 30 days (2) after immersion, using a profilometer, under the standard ISO 4287-1997. Data were analyzed with one-way analysis of variance (One-way ANOVA) and repeated measures analysis of variance (Repeated measures ANOVA) test (p < 0.05). Ultradent and VOCO had the higher baseline surface roughness values, while 3M presented the lower baseline values (p > 0.05). After 15 and 30 days of immersion, the 3M group still showed the minimum values of surface roughness (p < 0.05). In addition, the maximum roughness values were seen in group UC (Ultradent/Coca-Cola) (p < 0.05). The surface roughness of the PFS increased according to the period of immersion in some industrialized acidic beverages. This increase was specific to each commercial brand. Therefore, the 3M PFS presented the best performance before and after immersion in the beverages.


Pit and fissure sealants; Industrialized acidic beverages; Surface roughness; Dental materials

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Georgina Baca-Solano,Rosalía Contreras-Bulnes,Laura Emma Rodríguez-Vilchis,Bernardo Teutle-Coyotecatl,Ulises Velazquez-Enriquez. Effect of some industrialized acidic beverages on the roughness of pit and fissure sealants: an in vitro study. Journal of Clinical Pediatric Dentistry. 2022.doi:10.22514/jocpd.2022.031.


[1] Kassebaum NJ, Bernabé E, Dahiya M, Bhandari B, Murray CJL, Marcenes W. Global burden of untreated caries. Journal of Dental Research. 2015; 94: 650–658.

[2] Ramamurthy P, Rath A, Sidhu P, Fernandes B, Nettem S, Fee PA, et al. Sealants for preventing dental caries in primary teeth. The Cochrane Database of Systematic Reviews. 2022; 2: CD012981.

[3] World Health Association. What is the burden of oral disease?2022. Available at: (Accessed: 11 February, 2022).

[4] Cvikl B, Moritz A, Bekes K. Pit and fissure sealants a comprehensive review. Dentistry Journal. 2018; 6: 1–8.

[5] Wright JT, Crall JJ, Fontana M, Gillette EJ, Nový BB, Dhar V, et al. Evidence-based clinical practice guideline for the use of pit-and-fissure sealants. The Journal of the American Dental Association. 2016; 147: 672–682.e12.

[6] Gizani S. Pit and fissure sealants. Pit and Fissure Sealants. 2018; 72: 23–34.

[7] Naaman R, El-Housseiny AA, Alamoudi N. The use of pit and fissure sealants a literature review. Dentistry Journal. 2017; 5: 2–19.

[8] Wang L, D’Alpino PHP, Lopes LG, Pereira JC. Mechanical properties of dental restorative materials: relative contribution of laboratory tests. Journal of Applied Oral Science. 2003; 11: 162–167.

[9] Saads Carvalho T, Lussi A. Acidic beverages and foods associated with dental erosion and erosive tooth wear. Translated from the orginal title by Zohoori FV, Duckworth RM.1st ed. Karger: Basel. 2020.

[10] Kazak M, Tiryaki M, Turkes Basaran E, Benderli Gokce Y. Evaluating the effects of different beverages with daily consumption habits on the wear of restorative materials. Odontology. 2020; 108: 636–645.

[11] Karda B, Jindal R, Mahajan S, Sandhu S, Sharma S, Kaur R. To analyse the erosive potential of commercially available drinks on dental enamel and various tooth-coloured restorative materials—an in vitro study. Journal of Clinical and Diagnostic Research. 2016; 10: 117–121.

[12] Bagheri R, Burrow MF, Tyas M. Influence of food-simulating solutions and surface finish on susceptibility to staining of aesthetic restorative materials. Journal of Dentistry. 2005; 33: 389–398.

[13] Karatas O, Gul P, Gündoğdu M, Iskenderoglu DT. An evaluation of surface roughness after staining of different composite resins using atomic force microscopy and a profilometer. Microscopy Research and Technique. 2020; 83: 1251–1259.

[14] Tavangar M, Bagheri R, Kwon T, Mese A, Manton DJ. Influence of beverages and surface roughness on the color change of resin composites. Journal of Investigative and Clinical Dentistry. 2018; 9: e12333.

[15] International Standards Organization. ISO 4287:1997 Geometrical product specifications (GPS)—surface texture: profile method—terms, definitions and surface texture parameters. 2005. Available at: https: // (Accessed: 25 May 2022).

[16] Delaviz Y, Finer Y, Santerre JP. Biodegradation of resin composites and adhesives by oral bacteria and saliva: a rationale for new material designs that consider the clinical environment and treatment challenges. Dental Materials. 2014; 30: 16–32.

[17] Food and Drug Administration. Food and Drug Administration. 2022. Available at: (Accessed: 03 September, 2022).

[18] Badra VV, Faraoni JJ, Ramos RP, Palma-Dibb RG. Influence of different beverages on the microhardness and surface roughness of resin composites. Operative Dentistry. 2005; 30: 213–219.

[19] Briso A, Caruzo L, Guedes A, Catelan A, Santos PD. In vitro evaluation of surface roughness and microhardness of restorative materials submitted to erosive challenges. Operative Dentistry. 2011; 36: 397–402.

[20] Beltrami R, Colombo M, Cavada A, Panizzi S, Poggio C, Scribante A. Influence of enamel exposure to acidic drink on shear bond strength of different fissure sealants. Bioengineering. 2022; 9: 1–9.

[21] Erdemir U, Yildiz E, Eren MM, Ozel S. Surface hardness of different restorative materials after long-term immersion in sports and energy drinks. Dental Materials Journal. 2012; 31: 729–736.

[22] Curtin JA, Lu H, Milledge JT, Hong L, Peterson J. In vitro staining of resin composites by liquids ingested by children. Pediatric Dentistry. 2008; 30: 317–322.

[23] Ruivo MA, Pacheco RR, Sebold M, Giannini M. Surface roughness and filler particles characterization of resin-based composites. Microscopy Research and Technique. 2019; 82: 1756–1767.

[24] Skaria S, Berk KJ. Experimental dental composites containing a novel methacrylate-functionalized calcium phosphate component: evaluation of bioactivity and physical properties. Polymers. 2021; 13: 2095.

[25] Francois P, Fouquet V, Attal JP, Dursun E. Commercially available fluoride-releasing restorative materials: a review and a proposal for classification. Materials. 2020;13: 2313.

[26] Kobayashi TY, Rios D, Machado MADAM, Oliveira TM, Silva SMBD. A two-year clinical evaluation of fluoride and non-fluoride resin-based pit-and-fissure sealants. Brazilian Dental Journal. 2015; 26: 678–684.

[27] Martins GC, Meier MM, Loguercio AD, Reis A, Gomes JC, Gomes OM. Effects of adding barium-borosilicate glass to a simplified etch-and-rinse adhesive on radiopacity and selected properties. The Journal of Adhesive Dentistry. 2014; 16: 107–114.

[28] Correr GM, Bruschi RC, Baratto-Filho F, Correr-Sobrinho L, Sinhoreti MA, Puppin-Rontani RM. In vitro long-term degradation of aesthetic restorative materials in food-simulating media. Acta Odontologica Scandinavica. 2012; 70: 101–108.

[29] Lodi CS, Sassaki KT, Fraiz FC, Delbem ACB, Martinhon CCR. Evaluation of some properties of fermented milk beverages that affect the demineralization of dental enamel. Brazilian Oral Research. 2010; 24: 95–101.

[30] Tahmassebi JF, Duggal MS, Malik-Kotru G, Curzon MEJ. Soft drinks and dental health: a review of the current literature. Journal of Dentistry. 2006; 34: 2–11.

[31] Barac R, Gasic J, Trutic N, Sunaric S, Popovic J, Djekic P, et al. Erosive effect of different soft drinks on enamel surface in vitro: application of stylus profilometry. Medical Principles and Practice. 2015; 24: 451–457.

[32] İlday N, Bayindir YZ, Erdem V. Effect of three different acidic beverages on surface characteristics of composite resin restorative materials. Materials Research Innovations. 2010; 14: 385–391.

[33] Yap AUJ, Low JS, Ong LFKL. Effect of food—simulating liquids on surface characteristics of composite and polyacid-modified composite restoratives. Operative Dentistry. 2000; 25: 170–176.

[34] de Paula A, de Fúcio S, Alonso R, Ambrosano G, Puppin-Rontani R. Influence of chemical degradation on the surface properties of nano restorative materials. Operative Dentistry. 2014; 39: E109–E117.

[35] Elwardani G, Sharaf AA, Mahmoud A. Evaluation of colour change and surface roughness of two resin-based composites when exposed to beverages commonly used by children: an in-vitro study. European Archives of Paediatric Dentistry. 2019; 20: 267–276.

[36] Teutle-Coyotecatl B, Contreras-Bulnes R, Rodríguez-Vilchis LE, Scougall-Vilchis RJ, Velazquez-Enriquez U, Almaguer-Flores A, et al. Effect of surface roughness of deciduous and permanent tooth enamel on bacterial adhesion. Microorganisms. 2022; 10: 1701.

[37] Kielbassa AM, Ulrich I, Schmidl R, Schüller C, Frank W, Werth VD. Resin infiltration of deproteinised natural occlusal subsurface lesions improves initial quality of fissure sealing. International Journal of Oral Science. 2017; 9: 117–124.

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