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Original Research

Open Access

Effectiveness of pediatric rotary, rotary and reciprocating instrumentations on bacterial load reduction in primary molars: an ex vivo comparative study

  • Esra Oz1,*,
  • Berna Gulseren Timur2
  • Emel Sesli Cetin3
  • Goksel Bilir3

1Department of Pedodontics, Faculty of Dentistry, Suleyman Demirel University, 32040, Isparta, Turkey

2Kartal Oral and Health Center, 34880, Istanbul, Turkey

3Department of Microbiology, Faculty of Medicine, Suleyman Demirel University, 32040, Isparta, Turkey

DOI: 10.22514/jocpd.2023.009 Vol.47,Issue 2,March 2023 pp.30-39

Submitted: 21 September 2022 Accepted: 07 December 2022

Published: 03 March 2023

*Corresponding Author(s): Esra Oz E-mail:


This study aimed to evaluate the efficacy of intracanal Enterococcus faecalis reduction using pediatric rotary (EndoArt Pedo Kit Blue, EasyInSmile X-Baby and Denco Kids), rotary (ProTaper Next) and reciprocating (WaveOne Gold) file systems through microbiological analyses in primary molars. Seventy-five mandibular primary second molars were selected and divided into five instrumentation groups and a negative control group. After incubation, five roots were used to confirm biofilm formation on the root canals. Before and after instrumentation, bacterial samples were collected. The bacterial load reduction was statistically analyzed by using Kruskall-Wallis and Dunn post hoc tests at a significance level of 0.05. Denco Kids and EndoArt Pedo Kit Blue promoted higher bacterial reduction than EasyInSmile X-Baby systems. There was no difference in bacterial reduction between ProTaper Next rotary file systems and other groups. Among the single-file techniques, instrumentation with the Denco Kids rotary system showed a more significant bacterial load reduction than WaveOne Gold (p < 0.05). All systems used in the study reduced bacterial counts from root canals in primary teeth. Further studies are required to generate more information about the use of pediatric rotary file systems in clinics.


Endodontics; Enterococcus faecalis; Primary molars; Rotary files

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Esra Oz,Berna Gulseren Timur,Emel Sesli Cetin,Goksel Bilir. Effectiveness of pediatric rotary, rotary and reciprocating instrumentations on bacterial load reduction in primary molars: an ex vivo comparative study. Journal of Clinical Pediatric Dentistry. 2023. 47(2);30-39.


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