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Original Research

Open Access

Crystalline analysis of dental enamel by X-Ray diffraction on pediatric patients with chronic kidney disease

  • Israel Ceja Andrade1
  • Miguel Angel López Alvarez2,*,
  • Maria Isabel Hernández Rivas3
  • Carmen Celina Alonso Sánchez4

1Department of Physics, University of Guadalajara (CUCEI), 44430 Guadalajara, Jalisco, México

2Department of Mechanical Engineering, University of Guadalajara (CUCEI), 44430 Guadalajara, Jalisco, México

3Department of Odontology, University of Guadalajara (CUCS), 44430 Guadalajara, Jalisco, México 4Department of Clínicas, University of Guadalajara (CU Altos), 44430 Guadalajara, Jalisco, México

DOI: 10.22514/jocpd.2023.026 Vol.47,Issue 3,May 2023 pp.84-88

Submitted: 07 October 2022 Accepted: 03 February 2023

Published: 03 May 2023

*Corresponding Author(s): Miguel Angel López Alvarez E-mail:


This study aimed to analyze the crystalline structure of dental enamel in pediatric patients with chronic kidney disease (CKD) by X-ray diffraction (XRD). The six tested samples had a mineral composition similar to hydroxyapatite, according to sheet JCPDS(Joint Committee on Powder Diffraction Standards) card #09-0432, which is normally found in dentine, and presented a lower amount of whitlockites (Ca, Mg)3(PO4)2. Pattern phases showed an increase in organic matter and a decrease in inorganic matter. At an interval of approximately 2θ = 15.7 to 27.2, amorphous organic matter corresponding to hydrated glucose was found. The hydroxyapatite patterns in this study differed from that of dental enamel found on permanent teeth.


Kidney disease; Enamel defects; X-ray diffraction-analysis

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Israel Ceja Andrade,Miguel Angel López Alvarez,Maria Isabel Hernández Rivas,Carmen Celina Alonso Sánchez. Crystalline analysis of dental enamel by X-Ray diffraction on pediatric patients with chronic kidney disease. Journal of Clinical Pediatric Dentistry. 2023. 47(3);84-88.


[1] Andaloro C, Sessa C, Bua N, La Mantia I. Chronic kidney disease in children: assessment of oral health status. Dental and Medical Problems. 2018; 55: 23–28.

[2] Shady A, Nawras MM, Ahmed A. The oral and dental dealth status in children under haemodialysis. Interventions in Pediatric Dentistry Open Access Journal. 2019; 2: 145–155.

[3] Piekoszewska-Ziętek P, Olczak-Kowalczyk D, Pańczyk-Tomaszewska M, Gozdowski D. Developmental abnormalities of teeth in children with nephrotic syndrome. International Dental Journal. 2022; 72: 572–577.

[4] Silva TMC, Alves LAC, Garrido D, Watanabe A, Mendes FM, Ciamponi AL. Health and oral health-related quality of life of children and adolescents with chronic kidney disease: a cross-sectional study. Quality of Life Research. 2019; 28: 2481–2489.

[5] Collins AJ, Foley RN, Herzog C, Chavers BM, Gilbertson D, Ishani A, et al. Excerpts from the us renal data system 2009 annual data report. American Journal of Kidney Diseases. 2010; 55: A6–A7.

[6] Al Nowaiser A, Roberts G, Trompeter R, Wilson M, Lucas V. Oral health in children with chronic renal failure. Pediatric Nephrology. 2003; 18: 39–45.

[7] Martins C, Siqueira WL, Guimarães Primo LSS. Oral and salivary flow characteristics of a group of Brazilian children and adolescents with chronic renal failure. Pediatric Nephrology. 2008; 23: 619–624.

[8] Subramaniam P, Gupta M, Mehta A. Oral health status in children with renal disorders. Journal of Clinical Pediatric Dentistry. 2012; 37: 89–93.

[9] Lucas VS, Roberts GJ. Oro-dental health in children with chronic renal failure and after renal transplantation: a clinical review. Pediatric Nephrology. 2005; 20: 1388–1394.

[10] Casanova AJ, Medina CE, Casanova JF, Vallejos AA, Martínez EA, Loyola-Rodríguez JP, et al. Association between developmental enamel defects in the primary and permanent dentitions. European Journal of Pediatric Dentistry. 2011; 12: 155–158.

[11] Suga S. Enamel hypomineralization viewed from the pattern of progressive mineralization of human and monkey developing enamel. Advances in Dental Research. 1989; 3: 188–198.

[12] Salanitri S, Seow W. Developmental enamel defects in the primary dentition: aetiology and clinical management. Australian Dental Journal. 2013; 58: 133–140.

[13] Tariq A, Ansari MA, Memon Z. Developmental enamel defects: a review. Journal of the Pakistan Dental Association. 2013; 22: 241–250.

[14] Paine ML, Krebsbach PH, Chen LS, Paine CT, Yamada Y, Deutsch D, et al. Protein-to-protein interactions: criteria defining the assembly of the enamel organic matrix. Journal of Dental Research. 1998; 77: 496–502.

[15] Simmer JP, Papagerakis P, Smith CE, Fisher DC, Rountrey AN, Zheng L, et al. Regulation of dental enamel shape and hardness. Journal of Dental Research. 2010; 89: 1024–1038.

[16] Lacruz RS, Habelitz S, Wright JT, Paine ML. Dental enamel formation and implications for oral health and disease. Physiological Reviews. 2017; 97: 939–993.

[17] Jefrey AD. McDonald J and Avery’s Dentistry for the Child and Adolescent. 11th Edition. Elsevier: Amsterdan. 2021.

[18] Seow W. Developmental defects of enamel and dentine: challenges for basic science research and clinical management. Australian Dental Journal. 2014; 59: 143–154.

[19] Brook AH. Multilevel complex interactions between genetic, epigenetic and environmental factors in the aetiology of anomalies of dental development. Archives of Oral Biology. 2009; 54: S3–S17.

[20] Morales R, Guevara J. Structural alterations of teeth. Kiru. 2010; 7: 83–90.

[21] Koch MJ, Bührer R, Pioch T, Schärer K. Enamel hypoplasia of primary teeth in chronic renal failure. Pediatric Nephrology. 1999; 13: 68–72.

[22] Hanlie H, Liyun T, Tao J. The crystal characteristics of enamel and dentin by XRD method. Journal of Wuhan University of Technology-Materials Science Edition. 2006; 21: 9–12.

[23] Daculsi G, Menanteau J, Kerebel LM, Mitre D. Length and shape of enamel crystals. Calcified Tissue International. 1984; 36: 550–555.

[24] Dessombz A, Lignon G, Picaut L, Rouzière S, Berdal A. Mineral studies in enamel, an exemplary model system at the interface between physics, chemistry and medical sciences. Comptes Rendus Chimie. 2016; 19: 1656–1664.

[25] XUE J, ZAVGORODNIY AV, KENNEDY BJ, SWAIN MV, LI W. X-ray microdiffraction, TEM characterization and texture analysis of human dentin and enamel. Journal of Microscopy. 2013; 251: 144–153.

[26] LeGeros RZ. Calcium phosphates in oral biology and medicine. Monographs in Oral Science. 1991; 15: 1–201.

[27] LeGeros RZ, Trautz OR, Klein E, LeGeros JP. Two types of carbonate substitution in the apatite structure. Experientia. 1969; 25: 5–7.

[28] Angmar-Månsson B. A polarization microscopic and micro X-ray diffraction study on the organic matrix of developing human enamel. Archives of Oral Biology. 1971; 16: 147–156.

[29] Mahoney EK, Rohanizadeh R, Ismail FSM, Kilpatrick NM, Swain MV. Mechanical properties and microstructure of hypomineralised enamel of permanent teeth. Biomaterials. 2004; 25: 5091–5100.

[30] Whittaker DK. Structural variations in the surface zone of human tooth enamel observed by scanning electron microscopy. Archives of Oral Biology. 1982; 27: 383–392.

[31] De Menezes Oliveira MAH, Torres CP, Gomes-Silva JM, Chinelatti MA, De Menezes FCH, Palma-Dibb RG, et al. Microstructure and mineral composition of dental enamel of permanent and deciduous teeth. Microscopy Research and Technique. 2010; 73: 572–577.

[32] Zheng S, Deng H, Gao X, Cao C. Chemical composition and crystalline structure of hypoplastic primary dental enamel. International Journal of Oral-Medical Sciences. 2002; 1: 17–22.

[33] Gupta M, Gupta M, Abhishek. Oral conditions in renal disorders and treatment considerations—a review for pediatric dentist. The Saudi Dental Journal. 2015; 27: 113–119.

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