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Original Research

Open Access

Bisphenol A release from commercially available 3-dimensionally printed resins and human cell apoptosis to bisphenol A: an in-vitro study

  • Yun Sun Jung1,2,†
  • Sang Tae Ro1,2
  • Sang Wook Kang3,†
  • Hyeonjong Lee4
  • Jang Sun Lee5
  • Yong Kwon Chae1,2
  • Ko Eun Lee1
  • Hyo-Seol Lee1,6
  • Kyu Hwan Kwack7
  • Su Kang Kim8
  • Sung Chul Choi1,6
  • Ok Hyung Nam1,6,*,

1Department of Pediatric Dentistry, Kyung Hee University, College of Dentistry, Kyung Hee University Medical Center, 02447 Seoul, Republic of Korea

2Department of Dentistry, Graduate School, Kyung Hee University, 02447 Seoul, Republic of Korea

3Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, 02447 Seoul, Republic of Korea

4Department of Prosthodontics, Dental College, Yonsei University, 03722 Seoul, Republic of Korea

5Dio Implant Ortho Research & Design Center, 48058 Busan, Republic of Korea

6Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, 02447 Seoul, Republic of Korea

7Department of Oral Microbiology, College of Dentistry, Kyung Hee University, 02447 Seoul, Republic of Korea

8Department of Biomedical Laboratory Science, Catholic Kwandong University, 25601 Gangneung, Republic of Korea

DOI: 10.22514/jocpd.2023.027 Vol.47,Issue 3,May 2023 pp.89-95

Submitted: 12 December 2022 Accepted: 13 February 2023

Published: 03 May 2023

*Corresponding Author(s): Ok Hyung Nam E-mail: pedokhyung@gmail.com

† These authors contributed equally.

Abstract

Bisphenol A (BPA) from dental materials may be linked to children’s health issues. This study aimed to assess the release of BPA from commercially available 3-dimensional (3D)-printed resin materials and evaluate BPA-related apoptotic effects on human periodontal ligament cells and gingival fibroblasts. Commercially available 3D-printed resin materials for prosthodontic use were selected as follows: NextDent C&B MFH (3D Systems, Rock Hill, SC, USA), DIOnavi-P. MAX (Dio Co., Busan, Korea), and DIOnavi-Denture02 (Dio Co., Busan, Korea). Identical cuboidal samples (1 cm × 1 cm × 0.5 cm) were printed from the materials and cured. BPA release was assessed using liquid chromatography/mass spectrometry (LC/MS). In addition, human gingival fibroblasts and periodontal ligament cells were exposed to various BPA solutions based on the LC/MS results. Cell Counting kit-8 (CCK-8) and real-time polymerase chain reaction analyses were performed to evaluate BPA-related apoptotic effects. The LC/MS analysis confirmed that none of the 3D-printed resin materials released BPA after curing. Both human gingival fibroblasts and periodontal ligament cells showed lower viability after BPA exposure. Regarding apoptosis-related gene expression, Caspase10 (CASP10) expression in periodontal ligament cells was significantly different in the BPA solutions (p < 0.05). The expression of BAX and Capspase8 (CASP8) in gingival fibroblasts was significantly increased by BPA in a dose-dependent manner (p < 0.05). Within the limitations of this study, the 3D-printed resin materials were not found to release BPA. This finding implies that 3D-printed resin materials are not associated with potential BPA-related risks in children.


Keywords

3-dimensional printing; Bisphenol A; Children; Dental materials; Digital dentistry; Pediatric dentistry


Cite and Share

Yun Sun Jung,Sang Tae Ro,Sang Wook Kang,Hyeonjong Lee,Jang Sun Lee,Yong Kwon Chae,Ko Eun Lee,Hyo-Seol Lee,Kyu Hwan Kwack,Su Kang Kim,Sung Chul Choi,Ok Hyung Nam. Bisphenol A release from commercially available 3-dimensionally printed resins and human cell apoptosis to bisphenol A: an in-vitro study. Journal of Clinical Pediatric Dentistry. 2023. 47(3);89-95.

References

[1] Tian Y, Chen C, Xu X, Wang J, Hou X, Li K, et al. A review of 3D printing in dentistry: technologies, affecting factors, and applications. Scanning. 2021; 2021: 9950131.

[2] Kim N, Kim H, Kim IH, Lee J, Lee KE, Lee HS, et al. Novel 3D printed resin crowns for primary molars: in vitro study of fracture resistance, biaxial flexural strength, and dynamic mechanical analysis. Children. 2022; 9: 1445.

[3] Al-Halabi MN, Bshara N, Nassar JA, Comisi JC, Alawa L. Comparative assessment of novel 3D printed resin crowns versus direct celluloid crowns in restoring pulp treated primary molars. Journal of Evidence-Based Dental Practice. 2022; 22: 101664.

[4] Azimi P, Zhao D, Pouzet C, Crain NE, Stephens B. Emissions of ultrafine particles and volatile organic compounds from commercially available desktop three-dimensional printers with multiple filaments. Environmental Science & Technology. 2016; 50: 1260–1268.

[5] Mikołajewska K, Stragierowicz J, Gromadzińska J. Bisphenol A—application, sources of exposure and potential risks in infants, children and pregnant women. International Journal of Occupational Medicine and Environmental Health. 2015; 28: 209–241.

[6] Zulkifli S, Rahman AA, Kadir SHSA, Nor NSM. Bisphenol A and its effects on the systemic organs of children. European Journal of Pediatrics. 2021; 180: 3111–3127.

[7] Melzer D, Rice NE, Lewis C, Henley WE, Galloway TS. Association of urinary bisphenol A concentration with heart disease: evidence from NHANES 2003/06. PLoS One. 2010; 5: e8673.

[8] Braun JM, Hauser R. Bisphenol A and children’s health. Current Opinion in Pediatrics. 2011; 23: 233–239.

[9] Braun JM, Yolton K, Dietrich KN, Hornung R, Ye X, Calafat AM, et al. Prenatal bisphenol A exposure and early childhood behavior. Environmental Health Perspectives. 2009; 117: 1945–1952.

[10] Vandenberg LN, Hauser R, Marcus M, Olea N, Welshons WV. Human exposure to bisphenol A (BPA). Reproductive Toxicology. 2007; 24: 139–177.

[11] FUNG EYK, EWOLDSEN NO, ST. GERMAIN HA, MARX DB, MIAW C, SIEW C, et al. Pharmacokinetics of bisphenol A released from a dental sealant. The Journal of the American Dental Association. 2000; 131: 51–58.

[12] Van Landuyt KL, Nawrot T, Geebelen B, De Munck J, Snauwaert J, Yoshihara K, et al. How much do resin-based dental materials release?A meta-analytical approach. Dental Materials. 2011; 27: 723–747.

[13] Becher R, Wellendorf H, Sakhi AK, Samuelsen JT, Thomsen C, Bølling AK, et al. Presence and leaching of bisphenol A (BPA) from dental materials. Acta Biomaterialia Odontologica Scandinavica. 2018; 4: 56–62.

[14] Deviot M, Lachaise I, Högg C, Durner J, Reichl F, Attal J, et al. Bisphenol A release from an orthodontic resin composite: a GC/MS and LC/MS study. Dental Materials. 2018; 34: 341–354.

[15] Wu X, Dai S, Chen Y, He F, Xie H, Chen C. Reinforcement of dental resin composite via zirconium hydroxide coating and phosphate ester monomer conditioning of nano-zirconia fillers. Journal of the Mechanical Behavior of Biomedical Materials. 2019; 94: 32–41.

[16] Alamri A, Semlali A, Jacques É, Alanazi M, Zakrzewski A, Chmielewski W, et al. Long-term exposure of human gingival fibroblasts to cigarette smoke condensate reduces cell growth by modulating Bax, caspase-3 and p53 expression. Journal of Periodontal Research. 2015; 50: 423–433.

[17] Nam OH, Ro ST, Lee HW, Jeong J, Chae YK, Lee KE, et al. Evaluation of delphinidin as a storage medium for avulsed teeth. BMC Oral Health. 2023; 23: 21.

[18] Huang F, Chang Y, Lee S, Ho Y, Yang M, Lin H, et al. Bisphenol A exhibits cytotoxic or genotoxic potential via oxidative stress-associated mitochondrial apoptotic pathway in murine macrophages. Food and Chemical Toxicology. 2018; 122: 215–224.

[19] Kang SW, Park HJ, Ban JY, Chung JH, Chun GS, Cho JO. Effects of nicotine on apoptosis in human gingival fibroblasts. Archives of Oral Biology. 2011; 56: 1091–1097.

[20] Wuersching SN, Hickel R, Edelhoff D, Kollmuss M. Initial biocom-patibility of novel resins for 3D printed fixed dental prostheses. Dental Materials. 2022; 38: 1587–1597.

[21] Guerrero-Gironés J, López-García S, Pecci-Lloret MR, Pecci-Lloret MP, Rodríguez Lozano FJ, García-Bernal D. In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices. Journal of Dentistry. 2022; 123: 104163.

[22] Tomás-Catalá CJ, Collado-González M, García-Bernal D, Oñate-Sánchez RE, Forner L, Llena C, et al. Biocompatibility of new pulp-capping materials NeoMTA plus, MTA repair HP, and biodentine on human dental pulp stem cells. Journal of Endodontics. 2018; 44: 126–132.

[23] Wei X, Pan Y, Wang M, Wang Y, Lin H, Jiang L, et al. Comparative analysis of leaching residual monomer and biological effects of four types of conventional and CAD/CAM dental polymers: an in vitro study. Clinical Oral Investigations. 2022; 26: 2887–2898.

[24] Mourouzis P, Diamantopoulou E, Plastiras O, Samanidou V, Tolidis K. Elution of monomers from CAD-CAM materials and conventional resin composite in distilled water and artificial saliva. Operative Dentistry. 2022; 47: E241–E252.

[25] He J, Kopperud HM. Preparation and characterization of Bis-GMA-free dental composites with dimethacrylate monomer derived from 9,9-Bis[4-(2-hydroxyethoxy)phenyl]fluorene. Dental Materials. 2018; 34: 1003–1013.

[26] Jin G, Gu H, Jang M, Bayarsaikhan E, Lim J, Shim J, et al. Influence of postwashing process on the elution of residual monomers, degree of conversion, and mechanical properties of a 3D printed crown and bridge materials. Dental Materials. 2022; 38: 1812–1825.

[27] Bayarsaikhan E, Lim J-H, Shin S-H, Park KH, Park YB, Lee JH, et al. Effects of postcuring temperature on the mechanical properties and biocompatibility of three-dimensional printed dental resin material. Polymers. 2021; 13: 1180.

[28] Labrie D, Price RB, Sullivan B, Salazar AM, Gautam D, Stansbury JW, et al. Effect of thickness on the degree of conversion of two bulk-fill and one conventional posterior resin-based composites at high irradiance and high temporal resolution. Journal of the Mechanical Behavior of Biomedical Materials. 2022; 136: 105489.

[29] Groth C, Kravitz ND, Jones PE, Graham JW, Redmond WR. Three-dimensional printing technology. Journal of Clinical Orthodontics. 2014; 48: 475–485.

[30] Zhang J, Hu Q, Wang S, Tao J, Gou M. Digital light processing based three-dimensional printing for medical applications. International Journal of Bioprinting. 2020; 6: 242.

[31] Manapat JZ, Chen Q, Ye P, Advincula RC. 3D printing of polymer nanocomposites via stereolithography. Macromolecular Materials and Engineering. 2017; 302: 1600553.

[32] Yang M. The effects of cytotoxicity and genotoxicity induced by 2,2-bis[4-(acryloxypropoxy)phenyl]propane via caspases in human gingival fibroblasts. Toxicology and Industrial Health. 2014; 30: 755–764.

[33] Lai YL, Chen YT, Lee SY, Shieh TM, Hung SL. Cytotoxic effects of dental resin liquids on primary gingival fibroblasts and periodontal ligament cells in vitro. Journal of Oral Rehabilitation. 2004; 31: 1165–1172.

[34] Fehlberg S, Gregel CM, Göke A, Göke R. Bisphenol A diglycidyl ether-induced apoptosis involves Bax/Bid-dependent mitochondrial release of apoptosis-inducing factor (AIF), cytochrome c and Smac/DIABLO. British Journal of Pharmacology. 2003; 139: 495–500.

[35] Xu J, Osuga Y, Yano T, Morita Y, Tang X, Fujiwara T, et al. Bisphenol A induces apoptosis and G2-to-M arrest of ovarian granulosa cells. Biochemical and Biophysical Research Communications. 2002; 292: 456–462.


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