Effects of LED curing light on silver diamine fluoride penetration into dentin
1Department Pediatric Dentistry, College of Dentistry, New York University, New York, NY 10010, USA
2Department of Molecular Pathobiology, College of Dentistry, New York University, New York, NY 10010, USA
3Department of Epidemiology and Health Promotion, College of Dentistry, New York University, New York, NY 10010, USA
DOI: 10.22514/jocpd.2023.071 Vol.47,Issue 6,November 2023 pp.44-50
Submitted: 31 March 2023 Accepted: 23 May 2023
Published: 03 November 2023
This ex-vivo study investigated the effect of a light-emitting diode (LED) curing light on the depth of penetration of Silver Diamine Fluoride (SDF) into carious lesions. Twenty-four primary teeth with untreated caries lesions were allocated into groups and treated within 5 min after extraction: (1) n = 6 treated for 1 min with one drop of SDF followed by 10 sec rinse with tap water, (2) n = 6 treated for 10 sec with one drop of SDF and exposed to LED light for 20 sec (30 sec total SDF exposure) followed by 10 sec rinse with tap water, (3) n = 6 treated for 10 sec with one drop of SDF followed by a 10 sec rinse with tap water, (4) n = 3 untreated, and (5) n = 3 untreated but exposed to LED light for 20 sec. Samples were prepared, embedded, sectioned and silver penetration was measured using backscattered electron imaging in the scanning electron microscope and energy-dispersive X-ray spectroscopy analysis. Results were expressed as the average relative depth of penetration (%) = Ag depth/lesion depth × 100 from 5 sites in each lesion. Group means were compared using mixed model analysis. Mean ± standard deviation (SD) penetration was: 86.4 ± 20.7% in Group 1, 94.3 ± 13.7% in Group 2, and 26.7 ± 13.9% in Group 3. Groups 1 and 2 were statistically similar and different from Group 3 (p < 0.001). Groups 4 and 5 had no silver present. Use of LED light for 20 sec after 10 sec SDF application appears to facilitate silver penetration, similar to a 1 min SDF application. Clinical studies are needed to define the role of silver penetration in sustained caries arrest.
Silver diamine fluoride; Deciduous teeth; Caries arrest; LED curing light; Caries management
Yasmi O. Crystal,Sasan Rabieh,Malvin N Janal,Gretchen Cerezal,Bin Hu,Timothy G. Bromage. Effects of LED curing light on silver diamine fluoride penetration into dentin. Journal of Clinical Pediatric Dentistry. 2023. 47(6);44-50.
 Crystal YO, Marghalani AA, Ureles SD, Wright
JT, Sulyanto R, Divaris K, et al. Use of silver siamine fluoride for dental
caries management in children and adolescents, including those with special health
care needs. Pediatric Dentistry. 2017; 39: 135–145.
 Mei ML, Lo ECM, Chu CH. Arresting dentine caries with silver diamine fluoride: what’s behind it? Journal of Dental Research. 2018; 97: 751–758.
 Crystal YO, Niederman R. Silver diamine fluoride treatment considerations in children’s caries management. Pediatric Dentistry. 2016; 38: 466–471.
 Gao SS, Zhao IS, Hiraishi N, Duangthip D, Mei ML, Lo ECM, et al. Clinical trials of silver diamine fluoride in arresting caries among children: a systematic review. JDR Clinical & Translational Research. 2016; 1: 201–210.
 FDI World Dental Federation. WHO breakthrough: model list of essential medicines includes new section for dental preparations. 2021. Available at: https://www.fdiworlddental.org/who-list-essential-medicines-includes-new-section-dental-preparations (Accessed: 19 April 2022).
 Mei ML, Ito L, Cao Y, Lo ECM, Li QL, Chu CH. An ex vivo study of arrested primary teeth caries with silver diamine fluoride therapy. Journal of Dentistry. 2014; 42: 395–402.
 Fung MHT, Duangthip D, Wong MCM, Lo ECM, Chu CH. Randomized clinical trial of 12% and 38% silver diamine fluoride treatment. Journal of Dental Research. 2018; 97: 171–178.
 Duangthip D, Wong MCM, Chu CH, Lo ECM. Caries arrest by topical fluorides in preschool children: 30-month results. Journal of Dentistry. 2018; 70: 74–79.
 Paul B, Sierra MA, Xu F, Crystal YO, Li X, Saxena D, et al. Microbial population shift and metabolic characterization of silver diamine fluoride treatment failure on dental caries. PLOS ONE. 2021; 16: e0242396.
 Horst JA, Ellenikiotis H, Milgrom PL. UCSF protocol for caries arrest using silver diamine fluoride: rationale, indications and consent. Journal of the California Dental Association. 2016; 44: 16–28.
 Mabangkhru S, Duangthip D, Chu CH, Phonghanyudh A, Jirarattanasopha V. A randomized clinical trial to arrest dentin caries in young children using silver diamine fluoride. Journal of Dentistry. 2020; 99: 103375.
 Toopchi S, Bakhurji E, Loo CY, Hassan M. Effect of light curing on silver diamine fluoride in primary incisors: a microscopic ex vivo study. Pediatric Dentistry. 2021; 43: 44–49.
 Hassan M, Bakhurji E, AlSheikh R. Application of Er, Cr: YSGG laser versus photopolymerization after silver diamine fluoride in primary teeth. Scientific Reports. 2021; 11: 20780.
 Shortall AC, Price RB, MacKenzie L, Burke FJ. Guidelines for the selection, use, and maintenance of LED light-curing units—part II. British Dental Journal. 2016; 221: 551–554.
 Mei ML, Ito L, Chu CH, Lo EC, Zhang CF. Prevention of dentine caries using silver diamine fluoride application followed by Er: YAG laser irradiation: an in vitro study. Lasers in Medical Science. 2014; 29: 1785–1791.
 Li Y, Liu Y, Psoter WJ, Nguyen OM, Bromage TG, Walters MA, et al. Assessment of the silver penetration and distribution in carious lesions of deciduous teeth treated with silver diamine fluoride. Caries Research. 2019; 53: 431–440.
 Seto J, Horst JA, Parkinson DY, Frachella JC, DeRisi JL. Enhanced tooth structure via silver microwires following treatment with 38 percent silver diamine fluoride. Pediatric Dentistry. 2020; 42: 226–231.
 Sulyanto RM, Kang M, Srirangapatanam S, Berger M, Candamo F, Wang Y, et al. Biomineralization of dental tissues treated with silver diamine fluoride. Journal of Dental Research. 2021; 100: 1099–1108.
 Featherstone JD, Duncan JF, Cutress TW. A mechanism for dental caries based on chemical processes and diffusion phenomena during in-vitro caries simulation on human tooth enamel. Archives of Oral Biology. 1979; 24: 101–112.
 Harned HS, Hildreth Jr CL. The diffusion coefficient of silver nitrate in dilute aqueous solution at 25°. Journal of the American Chemical Society. 1951; 73: 3292–3293.
 Akram FE, El-Tayeb T, Abou-Aisha K, El-Azizi M. A combination of silver nanoparticles and visible blue light enhances the antibacterial efficacy of ineffective antibiotics against methicillin-resistant staphylococcus aureus (MRSA). Annals of Clinical Microbiology and Antimicrobials. 2016; 15: 48.
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