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Original Research

Open Access

Assessment of oral bacteria potentially associated with the mobile microbiome in children with congenital heart disease

  • Sermin Dicle Aksakal1
  • Yeliz Guven1,*,
  • Nursen Topcuoglu2
  • Guven Kulekcı̇2
  • Oya Aktoren1

1Department of Pedodontics, Faculty of Dentistry, Istanbul University, 34134 Istanbul, Turkey

2Department of Oral Microbiology, Faculty of Dentistry, Istanbul University, 34134 Istanbul, Turkey

DOI: 10.22514/jocpd.2024.026

Submitted: 11 May 2023 Accepted: 07 July 2023

Online publish date: 22 January 2024

*Corresponding Author(s): Yeliz Guven E-mail: yguven@istanbul.edu.tr

Abstract

In this case-control study, we aimed to investigate the specific oral pathogens potentially associated with the mobile microbiome in children with congenital heart disease (CHD). Caries, oral hygiene and gingival indices were evaluated in 20 children with CHD and a healthy control group, and venous blood samples and saliva were collected. Using quantitative polymerase chain reaction (qPCR), blood samples were analyzed for the presence of bacterial DNA to determine the mobile microbiome, and saliva samples were analyzed to identify and quantify target microorganisms, including Streptococcus mutans (Sm) and its serotype k (Smk), Fusobacterium. nucleatum (Fn), Porphyromonas gingivalis (Pg), Scardovia wiggsiae (Sw) and Aggregitibacter actinomycetemcomitans (Aa) and its JP2 clone (JP2). The findings were analyzed by Mann Whitney U, chi-square, Fisher’s exact and Spearman’s Correlation tests. Bacterial DNA was identified in two blood samples. No significant differences were found between the groups regarding the presence and counts of bacteria in saliva. However, the CHD group exhibited significantly lower caries and higher gingival index scores than the control group. The presence of Pg and Aa were significantly associated with higher gingival index scores. Sm and Smk counts were significantly correlated with caries experience. A positive correlation was found between Fn and total bacteria counts. In conclusion, the mobile microbiome, which has been proposed as a potential marker of dysbiosis at distant sites, was very rare in our pediatric population. The counts of target microorganisms which are potentially associated with the mobile microbiome did not differ in children with CHD and healthy children.


Keywords

Mobile microbiome; Oral microbiome; Congenital heart disease; qPCR


Cite and Share

Sermin Dicle Aksakal,Yeliz Guven,Nursen Topcuoglu,Guven Kulekcı̇,Oya Aktoren. Assessment of oral bacteria potentially associated with the mobile microbiome in children with congenital heart disease. Journal of Clinical Pediatric Dentistry. 2024.doi:10.22514/jocpd.2024.026.

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