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Original Research

Open Access

Adhesion of tooth fragment after trauma: effect of adhesion strategy and storage in the rescue box

  • Asli Kaya1
  • Blend Hamza2
  • Nadin Al-Haj Husain1,3
  • Kiren J. Mätzener1
  • Mutlu Özcan1,*,

1Clinic of Masticatory Disorders and Dental Biomaterials, Center of Dental Medicine, University of Zurich, 8032 Zurich, ZH, Switzerland

2Clinic of Orthodontics and Pediatric Dentistry, Center of Dental Medicine, University of Zurich, 8032 Zurich, ZH, Switzerland

3Department of Reconstructive Dentistry and Gerodontology, School of Dental Medicine, University of Bern, 3010 Bern, BE, Switzerland

DOI: 10.22514/jocpd.2024.030 Vol.48,Issue 2,March 2024 pp.19-25

Submitted: 09 July 2023 Accepted: 11 August 2023

Published: 03 March 2024

*Corresponding Author(s): Mutlu Özcan E-mail:


This study aims to investigate the impact of storage conditions for crown fragments (specifically, whether they were stored within a tooth rescue box or in tap water) on their adhesion to fractured teeth when subjected to two different adhesive systems (namely, total etch and self etch). Sixty maxillary premolars were sectioned to obtain tooth fragments. These fragments were stored briefly (2 hours) and reattached in the following groups: Group 1 (fragments stored in tooth rescue box and reattached with etch and rinse (E&R) technique), Group 2 (fragments stored in tap water and reattached with E&R technique), Group 3 (fragments stored in tooth rescue box and reattached with self-etch (SE) technique), and Group 4 (fragments stored in tap water and reattached SE technique). After reattachment, the bonded tooth fragments underwent thermal cycling (500 cycles, 5–55 ◦C) and bond strength testing using a universal testing machine. Two-way Analysis of Variance (ANOVA) and Tukey’s tests were used for bond strength comparison (p ≤ 0.05). A two-parameter Weibull distribution was conducted to evaluate the reliability of the storage medium and adhesion modality on bond strength. The results showed that measured shear bond values (MPa ± Standard deviation (SD); arranged in descending order) for each group were: Group 2 (Tap water/E&R = 6.5 ± 2.1), Group 1 (Rescue box/E&R = 6.0 ± 2.5), Group 4 (Tap water/E&R = 5.1 ± 2.8), and Group 3 (Rescue box/SE = 3.6 ± 3.2). Significant differences were found only between Groups 2 and 3 (p = 0.002). In conclusion, storing crown fragments in a tooth rescue box did not significantly affect the shear bond strength of the restored tooth. However, fragments reattached using the self-etch technique showed comparable shear bond strength but a higher rate of adhesive failures compared to the E&R technique.


Dental trauma; Pediatric dentistry; Adhesion; Adhesive resin; Dental materials; Storage medium; Tooth rescue box

Cite and Share

Asli Kaya,Blend Hamza,Nadin Al-Haj Husain,Kiren J. Mätzener,Mutlu Özcan. Adhesion of tooth fragment after trauma: effect of adhesion strategy and storage in the rescue box. Journal of Clinical Pediatric Dentistry. 2024. 48(2);19-25.


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