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Original Research

Open Access

Clinical and radiographic evaluation of regenerative endodontic procedures (REPs) with or without concentrated growth factor (CGF) as scaffolds for non-vital immature mandibular premolars

  • Yanfei Zhang1,2,3
  • Min Sheng2,3,*,

1Shaoxing Stomatological Hospital, 312000 Shaoxing, Zhejiang, China

2Department of Stomatology, Shaoxing Yuecheng People’s Hospital, 312000 Shaoxing, Zhejiang, China

3Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, 311201 Hangzhou, Zhejiang, China

DOI: 10.22514/jocpd.2024.090 Vol.48,Issue 4,July 2024 pp.168-175

Submitted: 02 July 2023 Accepted: 02 August 2023

Published: 03 July 2024

*Corresponding Author(s): Min Sheng E-mail:


This study aimed to perform clinical and radiographic investigations of the effect of regenerative endodontic procedures (REPs) with and without concentrated growth factor (CGF). Fifty-six non-vital and immature teeth from 56 patients were randomly categorized into two groups. Following chemical and mechanical preparation, REPs with and without CGF as a scaffold was induced in the blood clot (BLC) group and the CGF group. All patients were clinically and radiographically evaluated at 6-month and 12-month intervals to monitor their progress and treatment outcomes. When considering the total number of patients, the follow-up rate was 96.4% (54 out of 56 patients) over a 12-month period. Favorable clinical and radiographic outcomes were observed in 92.6% of patients (25 out of 27) in both the CGF and BLC groups; there were no significant differences between the two groups in these respects (p > 0.05). Notable differences were, however, observed in radiographic measurements relating to the development of root length and radiographic root area when compared between the CGF and BLC groups at both the 6-month and 12-month follow-up intervals (p < 0.05). REPs have been proven to represent a conservative and effective approach for promoting maturogenesis in non-vital and immature teeth. Furthermore, the incorporation of CGF as scaffolds holds promising potential for enhancing the desired biological outcomes of this regenerative technique. These findings highlight the clinical significance and potential benefits of CGF supplementation in REPs, further supporting its application in the field of endodontics.


Maturogenesis; Concentrated growth factor; Immature teeth; Revascularization

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Yanfei Zhang,Min Sheng. Clinical and radiographic evaluation of regenerative endodontic procedures (REPs) with or without concentrated growth factor (CGF) as scaffolds for non-vital immature mandibular premolars. Journal of Clinical Pediatric Dentistry. 2024. 48(4);168-175.


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