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Original Research

Open Access

Cytotoxicity of two fluoride-releasing adhesive tapes

  • Seo-Rin Jeong1,†
  • Yong Kwon Chae2,†
  • Ok Hyung Nam2,3
  • Tae-Young Park4
  • Jonghyun Shin5
  • Myeong-Kwan Jih6,*,

1Department of Orthodontics, School of Dentistry, Chosun University, 61452 Gwangju, Republic of Korea

2Department of Pediatric Dentistry, Kyung Hee University, College of Dentistry, Kyung Hee University Medical Center, 02447 Seoul, Republic of Korea

3Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, 02447 Seoul, Republic of Korea

4Department of Conservative Dentistry, School of Dentistry, Chosun University, 61452 Gwangju, Republic of Korea

5Department of Pediatric Dentistry, School of Dentistry, Pusan National University, 50612 Yangsan, Republic of Korea

6Department of Pediatric Dentistry, School of Dentistry, Chosun University, 61452 Gwangju, Republic of Korea

DOI: 10.22514/jocpd.2024.115 Vol.48,Issue 5,September 2024 pp.154-165

Submitted: 31 October 2023 Accepted: 11 January 2024

Published: 03 September 2024

*Corresponding Author(s): Myeong-Kwan Jih E-mail: mdenti@chosun.ac.kr

† These authors contributed equally.

Abstract

Sodium fluoride-polyvinyl alcohol (NaF-PVA) tape was developed to deliver fluoride to teeth by adding fluoride to polymer tape. Previous studies have demonstrated that tapes are effective and have antimicrobial properties. This study aimed to evaluate the cytotoxicity of two fluoride-releasing adhesive tapes. We investigated two polyvinyl alcohol (PVA) tapes: (i) a fluoride-PVA (F-PVA) tape, and (ii) a pullulan-incorporated F-PVA (PF-PVA) tape. The cytotoxicity test was conducted on human gingival fibroblasts (HGF) and human periodontal ligament (PDL) cells. Using an adhesive tape containing fluoride, we performed the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on these cells. Genetic analysis of the cells was performed to conduct a stability test on humans. In the MTT assay, PF-PVA had 66% greater cytotoxicity than control by PDL and 69% by HGF. F-PVA showed less cytotoxicity than PF-PVA by 29% in PDL and 33% in HGF. Gene ontology (GO) analysis and gene set enrichment analysis (GSEA) were performed as gene expression analyses. GO analysis indicated that PF-PVA displayed more expression changes of genes related to cytotoxicity than F-PVA. In addition, GSEA found more inflammatory response associations in PF-PVA than in F-PVA. MTT and genetic testing yielded comparable results.


Keywords

Fluoride; Polyvinyl alcohol tape; Pullulan; Cytotoxicity


Cite and Share

Seo-Rin Jeong,Yong Kwon Chae,Ok Hyung Nam,Tae-Young Park,Jonghyun Shin,Myeong-Kwan Jih. Cytotoxicity of two fluoride-releasing adhesive tapes. Journal of Clinical Pediatric Dentistry. 2024. 48(5);154-165.

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