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Original Research

Open Access

Evaluation of EDARADD, LPO and ACTN2 genes polymorphisms in children with dental caries compared to caries-free controls

  • Mohammad Hassan Lotfalizadeh1
  • Sam Shahedi2
  • Sepehr Kobravi3
  • Arman Shekari2
  • Yasin Nazari4
  • Zahra Pirmoradi5
  • Kasra Nazari5
  • Mehri Safari6
  • Forough Taheri7
  • Farzaneh lal Alizadeh8,*,

1North Khorasan University of Medical Sciences, 0098051 Bojnurd, Iran

2Faculty of Dentistry, Yerevan State Medical University, 0054 Yerevan, Armenia

3Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tehran Azad University, 0098021 Tehran, Iran

4Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, 0098021 Tehran, Iran

5School of International Education, Faculty of Stomatology, Zhengzhou University, 451450 Zhengzhou, Henan, China

6Department of Operative Dentistry, Dental School, Islamic Azad University of Isfahan branch, 0098031 Isfahan, Iran

7Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, 0098031 Isfahan, Iran

8Dental Research Center, Mashhad University of Medical Sciences, 0098051 Mashhad, Iran

DOI: 10.22514/jocpd.2024.135 Vol.48,Issue 6,November 2024 pp.152-160

Submitted: 01 December 2023 Accepted: 07 March 2024

Published: 03 November 2024

*Corresponding Author(s): Farzaneh lal Alizadeh E-mail: laelaf@mums.ac.ir

Abstract

Dental caries is a complex condition that results from a combination of genetic and environmental factors. Several genes have been found to play a role in teeth development and have been associated with various dental traits. In this study, our objective was to examine the potential correlation between the ectodysplasin a receptor (EDAR)-associated via death domain (EDARADD), Lactoperoxidase (LPO) and Actinin Alpha 2 (ACTN2) gene polymorphisms and susceptibility to dental caries. The study included a total of 600 participants, comprising 300 individuals with dental caries and 300 caries-free controls. The genotyping of the EDARADD (rs79233817), LPO (rs8178275) and ACTN2 (rs114880747) gene polymorphisms was performed using the tetra-primer amplification refractory mutation system Polymerase chain reaction (PCR) method. Individuals with dental caries were found to have a significantly higher frequency of the A allele (minor allele) for rs79233817 compared to controls. The specific single nucleotide polymorphism (SNP) (rs79233817) was associated with an increased risk of dental caries (DC) in both the co-dominant and dominant genetic models (p-value ˂ 0.05). In addition, the study’s findings revealed a significant association between the rs114880747 SNP and susceptibility to dental caries (p-value ˂ 0.05). A higher frequency of the A allele (minor allele) of rs114880747 was observed in patients compared to the healthy controls. It is also worth mentioning that there was no association between rs8178275 susceptibility to dental caries (p-value ˃ 0.05).It can be inferred that the EDARADD gene polymorphism (rs79233817) and ACTN2 gene polymorphism (rs114880747) potentially play a role in the genetic susceptibility to dental caries. To validate and delve deeper into these findings, it is necessary to conduct additional studies with larger sample sizes in diverse populations. This will help to establish the robustness of the results and further investigate the underlying mechanisms involved.


Keywords

Dental caries; EDARADD; ACTN2; LPO; Genetics; Diagnosis and treatment planning


Cite and Share

Mohammad Hassan Lotfalizadeh,Sam Shahedi,Sepehr Kobravi,Arman Shekari,Yasin Nazari,Zahra Pirmoradi,Kasra Nazari,Mehri Safari,Forough Taheri,Farzaneh lal Alizadeh. Evaluation of EDARADD, LPO and ACTN2 genes polymorphisms in children with dental caries compared to caries-free controls. Journal of Clinical Pediatric Dentistry. 2024. 48(6);152-160.

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