Article Data

  • Views 4338
  • Dowloads 262

Original Research

Open Access

The evaluation of the effects of saliva contamination on microhardness and fracture strength of different aged restorative materials

  • Asena Adaklı Durmaz1
  • Akif Demirel1,*,
  • Zeynep Ökte1

1Pediatric Dentistry Department, Faculty of Dentistry, Ankara University, 06560 Ankara, Turkey

DOI: 10.22514/jocpd.2025.111 Vol.49,Issue 5,September 2025 pp.168-177

Submitted: 22 October 2024 Accepted: 06 January 2025

Published: 03 September 2025

*Corresponding Author(s): Akif Demirel E-mail: akifdemirel@ankara.edu.tr

Abstract

Background: This research aimed to investigate the effects of saliva contamination on the microhardness and fracture strength of different types of aged restorative materials under in vitro conditions. Methods: 90 samples assigned to compomer, glass hybrid restorative (GHR), and conventional glass ionomer restoratives (CGIR) were prepared using round-shaped molds (diameter: 6 mm, depth: 2 mm). Samples in main groups were subdivided to simulate a saliva-contaminated (n = 15) and non-contaminated conditions/cases (n = 15). In saliva-contaminated subgroups, artificial saliva solution was applied to all surfaces of the molds, and restorative materials were placed. All samples were thermocycled with a temperature of 5–55 ◦C, 30 seconds dwell time and 5000 cycles for aging. The surface hardness and then fracture strength were measured and recorded. Statistical tests were performed with Kruskal-Wallis-H and Mann-Whitney U tests. The significance level was set at 0.05. Results: In both saliva-contaminated and non-saliva-contaminated samples, glass hybrid restorative showed the highest microhardness, while compomer provided the best fracture strength (p < 0.05). For compomer material, no significant difference was found in terms of hardness and fracture strength between saliva-contaminated and non-contaminated samples (p > 0.05). For glass hybrid restorative material, non-saliva contaminated samples showed significantly higher fracture strength (p < 0.05). In conventional glass ionomer material, non-saliva contaminated samples showed significantly higher microhardness values (p < 0.05). Conclusions: Within the limitations of this study, it is recommended that saliva contamination be prevented as much as possible in order not to adversely affect the fracture strength in glass hybrid restorations and microhardness values in conventional glass ionomer restorations.


Keywords

Compomer; Conventional glass ionomer; Fracture strength; Glass hybrid restorative; Microhardness; Saliva contamination


Cite and Share

Asena Adaklı Durmaz, Akif Demirel, Zeynep Ökte. The evaluation of the effects of saliva contamination on microhardness and fracture strength of different aged restorative materials. Journal of Clinical Pediatric Dentistry. 2025; 49(5): 168-177. doi: 10.22514/jocpd.2025.111

References

[1] Agel M, Alani A. The paediatric dentistry-restorative dentistry interface. British Dental Journal. 2022; 233: 475–482.

[2] Dhar V, Hsu KL, Coll JA, Ginsberg E, Ball BM, Chhibber S, et al. Evidence-based update of pediatric dental restorative procedures: dental materials. Journal of Clinical Pediatric Dentistry. 2015; 39: 303–310.

[3] Deprá MB, de Almeida JX, da Cunha Tde M, Lon LF, Retamoso LB, Tanaka OM. Effect of saliva contamination on bond strength with a hydrophilic composite resin. Dental Press Journal of Orthodontics. 2013; 18: 63–68.

[4] Shimazu K, Karibe H, Ogata K. Effect of artificial saliva contamination on adhesion of dental restorative materials. Dental Materials Journal. 2014; 33: 545–550.

[5] Cagetti MG, Campus G. The future of pediatric dentistry is now. Children. 2023; 10: 97.

[6] Ammann P, Kolb A, Lussi A, Seemann R. Influence of rubber dam on objective and subjective parameters of stress during dental treatment of children and adolescents—a randomized controlled clinical pilot study. International Journal of Paediatric Dentistry. 2013; 23: 110–115.

[7] Sunanda YL, Parvathaneni KP, Raju TBVG, Seshadri A, Dondapati GD. Effect of blood and artificial saliva contamination on marginal adaptation and sealing ability of different retrograde filling materials: a comparative analysis. Journal of Conservative Dentistry and Endodontics. 2024; 27: 743–749.

[8] Pandey R, Dixit N, Dixit KK, Roy S, Gaba C, Goyal C. Comparative evaluation of microleakage of mineral trioxide aggregate and Geristore root-end filling materials in different environments: an in vitro study. Journal of Conservative Dentistry. 2018; 21: 328–332.

[9] Slawinski D, Wilson S. Rubber dam use: a survey of pediatric dentistry training programs and private practitioners. Pediatric Dentistry. 2010; 32: 64–68.

[10] Ozer L, Ozalp N, Okte Z, Oztas D. Effects of saliva contamination on shear bond strength of compomer to dentin in primary teeth. American Journal of Dentistry. 2006; 19: 28–30.

[11] Beretta M, Federici Canova F, Gianolio A, Zaffarano L. Beyond the clinic: why new bioactive restorative materials have really changed Paediatric Dentistry. European Journal of Paediatric Dentistry. 2023; 24: 292–296.

[12] Krithikadatta J, Gopikrishna V, Datta M. CRIS guidelines (checklist for reporting in-vitro studies): a concept note on the need for standardized guidelines for improving quality and transparency in reporting in-vitro studies in experimental dental research. Journal of Conservative Dentistry. 2014; 17: 301–304.

[13] McKnight-Hanes C, Whitford GM. Fluoride release from three glass ionomer materials and the effects of varnishing with or without finishing. Caries Research. 1992; 26: 345–350.

[14] Ayatollahi MR, Yahya MY, Karimzadeh A, Nikkhooyifar M, Ayob A. Effects of temperature change and beverage on mechanical and tribological properties of dental restorative composites. Materials Science & Engineering. 2015; 54: 69–75.

[15] Fernandes IB, Pereira TS, Souza DS, Ramos-Jorge J, Marques LS, Ramos-Jorge ML. Severity of dental caries and quality of life for toddlers and their families. Pediatric Dentistry. 2017; 39: 118–123.

[16] Rodrigues JA, Casagrande L, Araújo FB, Lenzi TL, Mariath AAS. Restorative materials in pediatric dentistry, pediatric restorative dentistry. In Leal SC, Takeshita EM (eds.) Pediatric Restorative Dentistry (pp. 161–167). Springer International Publishing: Switzerland. 2019.

[17] Sahebalam R, Boruziniat A, Mohammadzadeh F, Rangrazi A. Effect of the time of salivary contamination during light curing on degree of conversion and microhardness of a restorative composite resin. Biomimetics. 2018; 3: 23.

[18] Çelik Ç, Bayraktar Y, Özdemir BE. Effect of saliva contamination on microleakage of open sandwich restorations. Acta stomatologica Croatica. 2020; 54: 273–282.

[19] Chaudhari RR, Srivastava HR, Raisingani D, Prasad AB, Chinchalkar RP, Gattani S, et al. Effect of saliva contamination on shear bond strength of self-etch adhesive system to dentin: an in vitro study. International Journal of Clinical Pediatric Dentistry. 2021; 14: 443–446.

[20] Nair P, Hickel R, Ilie N. Adverse effects of salivary contamination for adhesives in restorative dentistry. A literature review. American Journal of Dentistry. 2017; 30: 156–164.

[21] Taneja S, Kumari M, Bansal S. Effect of saliva and blood contamination on the shear bond strength of fifth-, seventh-, and eighth-generation bonding agents: an in vitro study. Journal of Conservative Dentistry. 2017; 20: 157–160.

[22] Pashley EL, Tao L, Mackert JR, Pashley DH. Comparison of in vivo vs. in vitro bonding of composite resin to the dentin of canine teeth. Journal of Dental Research. 1988; 67: 467–470.

[23] Aidaros NH, Abdou A. Effect of contamination of bulk-fill flowable resin composite with different contaminants during packing on its surface microhardness and compressive strength: in vitro study. BMC Oral Health. 2022; 22: 446.

[24] Handoko MW, Tjandrawinata R, Octarina. The effect of nanofilled resin coating on the hardness of glass ionomer cement. Scientific Dental Journal. 2020; 4: 97–100.

[25] Alqasabi SY, Sulimany AM, Almohareb T, Alayad AS, Bawazir OA. The effect of different coating agents on the microhardness, water sorption, and solubility of EQUIA Forte® HT. Coatings. 2024; 14: 751.

[26] Demirel A, Orhan AI, Büyüksungur A. The assessment of internal adaptation and fracture resistance of glass ionomer and resin-based restorative materials applied after different caries removal techniques in primary teeth: an in-vitro study. PeerJ. 2023; 11: e14825.

[27] Moshaverinia M, Navas A, Jahedmanesh N, Shah KC, Moshaverinia A, Ansari S. Comparative evaluation of the physical properties of a reinforced glass ionomer dental restorative material. Journal of Prosthetic Dentistry. 2019; 122: 154–159.


JOCPD Volume 50 Issue 5 cover
Current Issue

Vol.50, Issue 5, 03 September 2026

Table of contents
All Issues

Submission Turnaround Time

Top