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Original Research

Open Access

Nigella Sativa Oil as a Pulp Medicament for Pulpotomized Teeth: A Histopathological Evaluation

  • Omar OM1,*,
  • Khattab NM2
  • Khater DS2

1Department of Pediatric and Community Dentistry, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

2Department of Pediatric and Community Dentistry, Faculty of Dentistry, Minia University, Minia, Egypt.

DOI: 10.17796/jcpd.36.4.n6674435856q86w8 Vol.36,Issue 4,July 2012 pp.335-341

Published: 01 July 2012

*Corresponding Author(s): Omar OM E-mail: omaromo@yahoo.com

Abstract

Concerns about the safety of formocresol (FC) as a pulpotomy agent in Pediatric Dentistry have lead to the search of new capping medicaments. Indigenous plant medicines such as Nigella Sativa (NS) have been the focus of many researches. Therefore the purpose of this study was to investigate histo-pathologically the pulp response to NS oil and FC in dogs. Method: Forty teeth in 4 male dogs of undefined breed aging 12-14 months were used in this study. Coronal access cavities were performed on the upper and lower premolars so that both medicaments were tested in the same animal in alternate sides of the mouth. Four weeks after treatment the animals were sacrificed, paraffin sections were prepared for histological, histochemical and immuno-histochemical staining. Results: specimens in the NS group showed mild to moderate vasodilatation. Few specimens showed scattered inflammatory cell infiltration and the odontoblastic layer was continuous. While the FC group showed moderate to severe vasodilatation with high inflammatory cell infiltrate and degenerative changes. Conclusions: NS possesses an anti-inflammatory effect and the pulp maintains its vitality after its application, which could qualify its use as a pulp medicament for pulpotomized teeth in clinical practice.

Keywords

Nigella Sativa oil, formocresol, animal study, pulp tissue.

Cite and Share

Omar OM,Khattab NM,Khater DS. Nigella Sativa Oil as a Pulp Medicament for Pulpotomized Teeth: A Histopathological Evaluation. Journal of Clinical Pediatric Dentistry. 2012. 36(4);335-341.

References

1. Alacam A. Pulpal tissue changes following pulpotomies with formocresol, glutaraldehyde-calcium hydroxide, glutaraldehyde- zinc oxide eugenol pastes in primary teeth. J Pedod, 13:123–132, 1989.

2. Roberts JF Treatment of vital and non-vital primary molar teeth by one stage formocresol pulpotomy: clinical success and effect upon age at exfoliation. Int J Paediatr Dent, 6: 111–115,1996.

3. Guidelines on pulp therapy for primary and immature permanent teeth. American Academy of Pediatric Dentistry, Reference manual 32: 194–201, 2009.

4. Ketley CE, Goodman JR. Formocresol toxicity: is there a suitable alternative for pulpotomy of primary molars? Int J Paediatr Dent, 1:67–72, 1991.

5. Yamasaki M, Nakamura H, Kameyama Y Irritating effect of formocresol after pulpectomy in vivo. Int Endod J, 27: 245–251,1994,

6. Zarzar PA, Rosenblatt A, Takahashi CS, Takeuchi PL, Costa Junior LA Formocresol mutagenicity following primary tooth pulp therapy: an invivo study. J Dent, 31: 479–485, 2003.

7. Garcia-Godoy F. A 42 month clinical evaluation of glutaraldehyde pulpotomies in primary teeth. J Pedod, 10: 148–155, 1986.

8. Fei AL, Udein RD, Johnson R. A clinical study of ferric sulfate as a pulpotomy agent in primary teeth. J Pediatr Dent, 13: 327–332, 1991.

9. Dominguez MS, Witherspoon DE, Gutmann JL, Opperman LA. Histological and scanning electron microscopy assessment of various vital pulp therapy materials. J Endod, 29: 324–333, 2003.

10. Agamy HA, Bakry NS, Mounir MM, Avery DR. Comparison of mineral trioxide aggregate and formocresol as pulp capping agents pulpcapping agents in pulpotomized primary teeth. Pediatr Dent, 26: 302–309, 2004.

11. Regulatory situation of Herbal Medicines. Worlds Health Organization- Traditional medicine. A Worldwide Review. WHO, Geneva, Switzerland, pp. 1–5, 1998.

12. Khan MA. Chemical composition and medicinal properties of Nigella Staiva Linn. Inflammopharmacology, 7: 15–35, 1999.

13. Al-Bukhari MI (815) Sahih Al-Bukhari authentic narrations of prophet Mohammed; English translation MM Khan .Madinah Islamic University, Saudi Arabia, vol 7 p. 400, 1982.

14. Chakravarty N. Inhibition of histamine release from mast cells by Nigellone. Annals of Allergy, 70: 237–242, 1993.

15. Kandil O, Radwan NM, Hassan AB, Amer AM, El-Banna HA, Amer WM. Extracts and fractions of nigella sativa exhibit antimicrobial and immunological activities. FASEB J, 8: A 951, 1994.

16. Al-Ghamdi MS . The ant inflammatory, analgesic and antipyretic activity of nigella sativa. J Ethnopharmacol, 76: 45–48, 2001.

17. El-Kamali HH, Ahmed AH, Mohamed AS, Yahia AA, El_tayeh IH, Ali AA. Antibacterial properties of essential oils from nigella sativa seeds, cymbopogon citrates leaves and Pulicaria undulate aerial parts. Fitoterpia, 69: 77–78, 1998.

18. Ali B and Blunden G. Pharmacological and toxicological properties of Nigella sativa, Phytother Res, 17: 299–305, 2003.

19. Abu-Zinadah O: Using Nigella sativa Oil to Treat and Heal Chemical Induced Wound of Rabbit Skin. JKAU: Sci., 21(2): 335–346, 2009.

20. Awad EM. In vitro decreases of the fibrinolytic potential of cultured human fibrosarcoma cell line, HT1080, by Nigella sativa oil. Phytomedicine, Jan; 12(1-2): 100–7, 2005.

21. Lotfy A. and Zayed M.: Immunohistochemical study of the effect of nigella sativa L extract on chemotherapy induced oral mucositis in albino rats. Cairo Dental Journal, 25 (2): 159–166, 2009.

22. Heyeraas KJ, Sveen OB, Mjor IA. Pulp-dentin bioloy in restorative dentistry. Part 3: pulpal inflammation and its sequelae. Quint Int, 32: 611–625, 2001.

23. Murray PE, Garcia-Godoy F. Comparison of the clinical and pre-clinical biocompatibility testing of dental materials are the ISO usage tests meaningful? J Biomed Mater Res, 81: 51–58, 2007.

24. Mansour M, Tornhamre S: Inhibition of 5-lipoxygenase and leukotriene C4 synthase in human blood cells by thymoquinone. J Enzyme Inhib Med Chem, 19: 431–436, 2004.

25. El-Tawil SB, El-Dokki NA, Aly ZH. Comparative evaluation of Jojoba oil versus Formocresol pulpotomy in primary molars- in vitro study. Pakistan Oral Dental J, 29: 85–92, 2009.

26. Salako N, Joseph B, Ritwik P, Salonen J, John P, Junaid TA. Comparison of bioactive glass, mineral trioxide aggregate, ferric sulfate, and formocresol as pulpotomy agents in rat molar. Dent Traumatol, 19: 314–320, 2003.

27. Ranly DM. Pulpotomy therapy in primary teeth: new modalities for old rationales. Pediatr Dent, 16: 403–409, 1994.

28. Kitasako Y, Inokoshi S, Tagami J. Effects of direct pulp capping techniques on short term response of mechanically exposed pulps. J Dent 27: 257–263, 1999.

29. Costa C, A,Teixeira H, Fontana U. Response of human pulps capped with a self-etching adhesive system. Dent Mater, 17: 230–240, 2001.

30. Khattab NM, Omar OM. An investigation on the relative efficacy of Nigella sativa oil compared to formocresol as pulp medicaments in non-vital primary molars pulpotomies. Egypt Dent J, 52: 169–178, 2006.

31. Halwani E. Antibacterial activity of Thymoquinone and Thymohydroquinone of Nigella sativa L. and their interaction with some antibiotics. Advances in Biological Research, 3: 148–152, 2009.

32. Murray P, Kitasako Y, Tagami J, Windsor L, Smith A: Hierarchy of variables correlated to odontoblast like cells numbers following pulp capping. J Dent, 30: 297–304, 2002.

33. Endel E and Gerdes J. The ki-67 protein: fascinating forms of an unknown function. Exp Cell Res, 257: 231–237, 2000.

34. Fitzgerald M, Chiego Jr. D, Heys D. Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Arch Oral Biol, 35: 707–715, 1990.

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